Transcript: Mouse XM_006505198.3

PREDICTED: Mus musculus PHD finger protein 14 (Phf14), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Phf14 (75725)
Length:
3203
CDS:
426..3125

Additional Resources:

NCBI RefSeq record:
XM_006505198.3
NBCI Gene record:
Phf14 (75725)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006505198.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000177584 CCATCAGACAAACAGTTAATT pLKO.1 693 CDS 100% 15.000 21.000 N Phf14 n/a
2 TRCN0000019309 CGCATGATTCAAATTCAGGAA pLKO.1 2289 CDS 100% 2.640 3.696 N PHF14 n/a
3 TRCN0000312505 CGCATGATTCAAATTCAGGAA pLKO_005 2289 CDS 100% 2.640 3.696 N PHF14 n/a
4 TRCN0000219623 GGCCTTACTTGGCCGAATTAC pLKO.1 2453 CDS 100% 13.200 10.560 N Phf14 n/a
5 TRCN0000219622 ACAGTTCATGAAGGTTGTTAT pLKO.1 1437 CDS 100% 13.200 9.240 N Phf14 n/a
6 TRCN0000198236 GACACCTGTAAACTGCATTAT pLKO.1 2634 CDS 100% 13.200 9.240 N Phf14 n/a
7 TRCN0000182147 CGAGCACCTAAGGAAAGGAAA pLKO.1 2505 CDS 100% 4.950 3.465 N Phf14 n/a
8 TRCN0000181846 GCTTTGCTAGAACTGGAGTTT pLKO.1 1747 CDS 100% 4.950 3.465 N Phf14 n/a
9 TRCN0000222119 GCAATGATGAAGATCATAGTA pLKO.1 1165 CDS 100% 0.563 0.788 N PHF14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006505198.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07464 pDONR223 100% 88.7% 94% None (many diffs) n/a
2 ccsbBroad304_07464 pLX_304 0% 88.7% 94% V5 (many diffs) n/a
3 TRCN0000476554 CCACGAACTAGCCATATCATCTGC pLX_317 15.5% 88.7% 94% V5 (many diffs) n/a
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