Construct: ORF ccsbBroad304_07473
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002775.1_s304c1, BRDN0000407408
- Derived from:
- ccsbBroadEn_07473
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECE2 (9718)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07473
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 110599564 | EEF1AKMT4 | EEF1A lysine methyltransfer... | NM_032331.3 | 99.8% | 99.6% | 301C>T |
| 2 | human | 110599583 | EEF1AKMT4-ECE2 | EEF1AKMT4-ECE2 readthrough | NM_014693.4 | 25% | 19.5% | (many diffs) |
| 3 | mouse | 107522 | Ece2 | endothelin converting enzyme 2 | NM_025462.2 | 85.3% | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 831
- ORF length:
- 765
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ctctccaggg gcaggtaggg cgcctccgga gttaccggag cggaactgcg 121 ggtaccgcga agtcgagtac tgggatcagc gctaccaagg cgcagccgat tctgccccct 181 acgattggtt cggggacttc tcctccttcc gtgccctcct agagccggag ctgcggcccg 241 aggaccgtat ccttgtgcta ggttgcggga acagtgccct gagctacgag ctgttcctcg 301 gaggcttccc taatgtgacc agtgtggact actcatcagt cgtggtggct gccatgcagg 361 ctcgctatgc ccatgtgccg cagctgcgct gggagaccat ggatgtgcgg aagctggact 421 tccccagtgc ttcttttgat gtggtgctcg agaagggcac gctggatgcc ctgctggctg 481 gggaacgaga tccctggacc gtgtcctctg aaggtgtcca cactgtggac caggtgttga 541 gtgaggtgag ccgcgtgctt gtccctggag gccggtttat ctcaatgact tctgctgccc 601 cccactttcg gaccagacac tatgcccaag cctattatgg ctggtccctg aggcatgcta 661 cctatggcag cggtttccac ttccatctct acctcatgca caagggcggg aagctcagtg 721 tggcccagct ggctctgggg gcccaaatcc tctcaccccc cagacctccc acctcacctt 781 gcttccttca ggactcagat catgaggact tccttagtgc cattcagctc tgcccaactt 841 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 901 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta