Construct: ORF ccsbBroad304_07480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015045.1_s304c1, BRDN0000462015
- Derived from:
- ccsbBroadEn_07480
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHACTR2 (9749)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07480
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9749 | PHACTR2 | phosphatase and actin regul... | NM_014721.3 | 99.9% | 99.8% | 590C>T |
| 2 | human | 9749 | PHACTR2 | phosphatase and actin regul... | NM_001100164.2 | 98.1% | 97.5% | (many diffs) |
| 3 | human | 9749 | PHACTR2 | phosphatase and actin regul... | NM_001100166.2 | 87.3% | 87.3% | 420_421ins240 |
| 4 | human | 9749 | PHACTR2 | phosphatase and actin regul... | NM_001100165.2 | 85.7% | 85.2% | (many diffs) |
| 5 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | XM_006512670.3 | 84.2% | 82.3% | (many diffs) |
| 6 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | XM_006512669.3 | 82.8% | 80.4% | (many diffs) |
| 7 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | XM_006512665.2 | 76.2% | 74.1% | (many diffs) |
| 8 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | XM_006512673.3 | 75.8% | 73.7% | (many diffs) |
| 9 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | XM_006512666.1 | 75.6% | 73.6% | (many diffs) |
| 10 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | NM_001195066.1 | 74.4% | 72.7% | (many diffs) |
| 11 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | XM_006512667.2 | 73.6% | 71.9% | (many diffs) |
| 12 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | NM_001195096.1 | 73.1% | 71% | (many diffs) |
| 13 | mouse | 215789 | Phactr2 | phosphatase and actin regul... | NM_001033257.4 | 72.5% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1971
- ORF length:
- 1902
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacaacgcc gttgacggac tggacaaagc ttctatagca aactcagatg 121 gccccacagc aggttcccaa acacctccct tcaaaagaaa ggggaaacta tccaccattg 181 gtaaaatctt taagccttgg aaatggagga aaaagaagac cagcgacaaa tttagagaaa 241 cctcggcagt attagaaagg aagatatcca cacgacaaag tagagaggag ctgataagaa 301 ggggagtgct taaggaattg cctgatcaag atggagatgt aacagttaac tttgaaaatt 361 caaacgggca catgataccc atcggagagg aatctacccg agaggaaaat gtagtaaagt 421 ctgaagaagg taatggctct gtatctgaaa aaacaccacc tctggaggaa caggcagaag 481 ataagaaaga gaacactgaa aaccactctg aaacaccggc agctcctgct ctacctcctt 541 ctgctcctcc taagcctaga cccaaaccta aacccaaaaa atcacctgtg cctccgaaag 601 gggccactgc tggggccagc cacaaaggtg atgaagtgcc tcccattaaa aaaaatatca 661 aggctcctgg taagcaggcc cccgtccctc cacccaagcc agcaagccga aacacgaccc 721 gagaggctgc tggctcctct cattcaaaaa aaacaactgg ctctaaagca tcagcttcgc 781 catccacttc atccacctca tctcgtccca aagcttcaaa ggagacagtt tctagcaaag 841 cagggacagt ggggaccacc aagggcaaga gaaaaactga caagcagcca ataacttctc 901 acctgtcctc agacacaaca acttctggca catccgacct gaaaggagag cctgcagaga 961 ccagagtgga gagtttcaaa ctcgaacaga ctgtccctgg agctgaggag cagaacacag 1021 gcaaattcaa gtccatggtc cctccacccc ctgtggctcc agcaccttct cctctggccc 1081 cccctctccc tcttgaggat cagtgcatta ctgcctcaga cactccagtt gtcctcgtca 1141 gcgttggagc tgacctgccc gtctctgcct tagacccaag tcagcttctt tgggctgaag 1201 agccgacgaa cagaaccact ctctactcag gcactggctt aagtgttaac agagaaaatg 1261 caaaatgttt cacaaccaaa gaggagctgg ggaagacagt gcctcagcta ctgactcctg 1321 ggctgatggg cgaatcttca gaatccttta gtgcctcaga agatgaaggc cacagggaat 1381 accaagccaa tgactctgac tcggacgggc ctatcttgta caccgatgat gaggacgaag 1441 acgaagatga ggatggcagt ggagaaagtg ctttggcaag taaaatacgc cggagggata 1501 ctcttgctat caaacttggc aacagaccat ctaagaaaga actagaggac aaaaacatct 1561 tgcagcgtac atctgaagaa gagaggcagg aaatccgaca acaaattgga accaagttag 1621 tcaggaggct gagccagagg cccacaactg aagaattaga gcaaagaaac atcctaaaac 1681 aaaagaatga agaagaggaa caagaagcaa aaatggaact taaacgcaga ctcagcagaa 1741 agctcagcct gagacccaca gtggcagagc tacaagctcg aaggatcctg cgatttaacg 1801 agtatgtaga agtcacggat tctcctgact atgaccgccg agcagacaag ccctgggcca 1861 ggttaacacc tgcagacaag gcagcaataa ggaaagaact aaatgaattt aaaagcacag 1921 aaatggaagt tcatgaagag agtcgacagt ttacaaggtt tcatcgtcca ttgccaactt 1981 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2041 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta