Construct: ORF ccsbBroad304_07507

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018054.1_s304c1, BRDN0000409653
Derived from:
ccsbBroadEn_07507
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SV2B (9899)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07507
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9899 SV2B synaptic vesicle glycoprote... NM_001323031.2 99.9% 100% 279C>T
2 human 9899 SV2B synaptic vesicle glycoprote... NM_001323032.3 99.9% 100% 279C>T
3 human 9899 SV2B synaptic vesicle glycoprote... NM_001323037.3 99.9% 100% 279C>T
4 human 9899 SV2B synaptic vesicle glycoprote... NM_001323038.3 99.9% 100% 279C>T
5 human 9899 SV2B synaptic vesicle glycoprote... NM_001323039.3 99.9% 100% 279C>T
6 human 9899 SV2B synaptic vesicle glycoprote... NM_014848.7 99.9% 100% 279C>T
7 human 9899 SV2B synaptic vesicle glycoprote... XM_005254998.3 99.9% 100% 279C>T
8 human 9899 SV2B synaptic vesicle glycoprote... XM_017022761.1 99.9% 100% 279C>T
9 human 9899 SV2B synaptic vesicle glycoprote... XM_017022762.1 99.9% 100% 279C>T
10 human 9899 SV2B synaptic vesicle glycoprote... NM_001323034.3 90.1% 90.1% 279C>T;1506_1507ins201
11 human 9899 SV2B synaptic vesicle glycoprote... NM_001323033.3 83.6% 83.7% 279C>T;450_451ins333
12 human 9899 SV2B synaptic vesicle glycoprote... NM_001323036.3 78.8% 78.3% (many diffs)
13 human 9899 SV2B synaptic vesicle glycoprote... NM_001167580.3 77.8% 77.8% 0_1ins453
14 human 9899 SV2B synaptic vesicle glycoprote... NM_001323040.3 77.8% 77.8% 0_1ins453
15 mouse 64176 Sv2b synaptic vesicle glycoprote... NM_001109753.1 89.4% 94.8% (many diffs)
16 mouse 64176 Sv2b synaptic vesicle glycoprote... NM_153579.4 89.4% 94.8% (many diffs)
17 mouse 64176 Sv2b synaptic vesicle glycoprote... XM_006541059.3 89.4% 94.8% (many diffs)
18 mouse 64176 Sv2b synaptic vesicle glycoprote... XM_006541060.3 89.4% 94.8% (many diffs)
19 mouse 64176 Sv2b synaptic vesicle glycoprote... XM_006541061.2 89.4% 94.8% (many diffs)
20 mouse 64176 Sv2b synaptic vesicle glycoprote... XM_017312210.1 89.4% 94.8% (many diffs)
21 mouse 64176 Sv2b synaptic vesicle glycoprote... XM_017312212.1 89.4% 94.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2115
ORF length:
2049
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tgactacaag tatcaggaca attatggggg ctatgctccc agtgatggct
121attaccgcgg caatgagtcc aacccagaag aagatgcaca gagtgatgtc accgaaggcc
181atgatgagga agacgagatc tatgagggcg agtaccaggg tatccctcac ccagatgatg
241tcaaggccaa gcaggccaag atggcgccct ccagaatgga cagccttcgg ggccagacag
301acctgatggc tgagaggctg gaagatgagg agcagttggc ccatcagtac gagaccatca
361tggatgagtg tggccatggc cgcttccagt ggatcctctt tttcgtcttg ggtttggccc
421tgatggccga tggggtggaa gtgttcgtgg tgagttttgc cctgcccagt gcagagaagg
481acatgtgtct gtccagttcc aaaaaaggaa tgctagggat gatagtctac ttgggaatga
541tggcgggcgc cttcatcctg ggaggcctgg ctgataagct gggaaggaag cgagtcctca
601gcatgtctct ggccgtcaat gcctccttcg cctccctctc ttccttcgtg cagggatatg
661gagccttcct cttctgccga ctcatctcag gcatcggtat tgggggtgct ctaccgattg
721tttttgccta tttttctgaa ttcttgtctc gggagaagcg aggagaacac ctcagttggc
781tgggcatctt ctggatgact gggggcctgt acgcatctgc catggcctgg agcatcatcc
841cacactatgg ctggggcttc agcatgggga ccaattacca cttccatagc tggagagtgt
901ttgtcatcgt ctgtgctctg ccctgcaccg tgtccatggt ggccctgaag ttcatgccag
961agagcccaag gtttctgcta gagatgggca aacatgatga agcctggatg attctcaagc
1021aagtccatga caccaacatg agagctaagg ggaccccaga gaaagtgttc acggtttcca
1081acatcaaaac tcccaagcaa atggatgaat tcattgagat ccaaagttca acaggaacct
1141ggtaccagcg ctggctggtc agattcaaga ccattttcaa gcaggtctgg gataatgccc
1201tgtactgtgt gatggggccc tacagaatga atacactgat tctggccgtg gtttggtttg
1261ccatggcatt cagttactat ggactgacag tttggtttcc tgatatgatc cgctattttc
1321aagatgaaga atacaagtct aaaatgaagg tgttttttgg tgagcatgtg tacggcgcca
1381caatcaactt cacgatggaa aatcagatcc accaacatgg gaaacttgtg aatgataagt
1441tcacaagaat gtactttaaa catgtactct ttgaggacac attctttgac gagtgctatt
1501ttgaagacgt aacatcaaca gatacctact tcaaaaattg taccattgaa tcaaccatct
1561tttacaacac agacctctac gagcacaagt tcatcaactg tcggtttatc aactccacct
1621tcctggagca gaaggagggc tgccacatgg acttggagca agataatgac ttcctgattt
1681acctcgtcag cttcctgggc agcctgtctg tcttacccgg gaacatcatt tctgccctgc
1741tcatggatag aattggaagg ctcaagatga ttggtggctc catgctaatc tctgcagtct
1801gctgcttctt cctgtttttt ggcaacagtg agtctgcaat gatcggctgg cagtgcctgt
1861tctgtgggac aagcattgca gcctggaatg ctctggatgt gatcacagtg gagctgtatc
1921ccaccaacca gagagcaaca gccttcggca ttctcaatgg attatgcaaa tttggcgcca
1981tcctgggaaa caccatcttt gcttcttttg ttgggataac caaagtggtc cccatccttc
2041tggctgctgc ttctctggtt gggggtggcc tgattgccct tcgactgcca gagactcgag
2101aacaggtcct gatgagccca actttcttgt acaaagtggt tggtaagcct atccctaacc
2161ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2221cgacaatcaa cctctggatt a

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