Transcript: Mouse XM_017312212.1

PREDICTED: Mus musculus synaptic vesicle glycoprotein 2 b (Sv2b), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Sv2b (64176)
Length:
5355
CDS:
603..2654

Additional Resources:

NCBI RefSeq record:
XM_017312212.1
NBCI Gene record:
Sv2b (64176)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017312212.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000112388 CGCCACGATCAACTTTACCAT pLKO.1 1913 CDS 100% 3.000 4.200 N Sv2b n/a
2 TRCN0000419829 AGTTCATCAACTGTCGGTTTA pLKO_005 2125 CDS 100% 10.800 7.560 N SV2B n/a
3 TRCN0000112385 CCCAAATCTAAACCAAACTAA pLKO.1 4228 3UTR 100% 5.625 3.938 N Sv2b n/a
4 TRCN0000112386 GCCTGGATGATTCTCAAACAA pLKO.1 1539 CDS 100% 5.625 3.938 N Sv2b n/a
5 TRCN0000112389 CACCAATATGAGACCATCATT pLKO.1 879 CDS 100% 5.625 3.375 N Sv2b n/a
6 TRCN0000112387 GCTCTGAAGTTCATGCCAGAA pLKO.1 1479 CDS 100% 4.050 2.430 N Sv2b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017312212.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07507 pDONR223 100% 89.4% 94.8% None (many diffs) n/a
2 ccsbBroad304_07507 pLX_304 0% 89.4% 94.8% V5 (many diffs) n/a
3 TRCN0000471954 TTATTTTCTAGTAGTGGCCCTGGC pLX_317 18.7% 89.4% 94.8% V5 (many diffs) n/a
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