Construct: ORF ccsbBroad304_07517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011552.1_s304c1, BRDN0000405686
- Derived from:
- ccsbBroadEn_07517
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR1 (9948)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07517
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9948 | WDR1 | WD repeat domain 1 | NM_017491.5 | 99.9% | 99.8% | 553A>G |
| 2 | human | 9948 | WDR1 | WD repeat domain 1 | XM_017008880.2 | 91.9% | 91.8% | 553A>G;635_793del |
| 3 | human | 9948 | WDR1 | WD repeat domain 1 | NM_005112.5 | 76.8% | 76.8% | 137_138ins420 |
| 4 | mouse | 22388 | Wdr1 | WD repeat domain 1 | NM_011715.2 | 87.6% | 95% | (many diffs) |
| 5 | mouse | 22388 | Wdr1 | WD repeat domain 1 | XM_006503863.3 | 77.2% | 82.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1884
- ORF length:
- 1818
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtacgagatc aagaaggtgt tcgccagcct cccgcaggtg gagaggggcg 121 tctccaagat catcggcggc gaccctaagg gcaacaattt tctgtacacc aatggaaagt 181 gcgtcatcct aaggaacatc gacaacccag cccttgctga catctacaca gagcacgccc 241 atcaggtggt ggtggccaag tatgcgccca gcggattcta cattgcctcc ggagatgtgt 301 ctgggaagct gaggatctgg gataccacgc agaaggagca cctgttgaag tatgagtacc 361 agcctttcgc tgggaagatc aaagacattg cttggactga agacagtaag aggatcgccg 421 tggtcgggga aggaagggag aagtttggag cagtcttcct ctgggatagt ggctcttctg 481 tgggcgagat tacaggacac aacaaagtca tcaacagcgt ggacatcaag cagagccggc 541 cataccggct ggccacggga agcgatgata actgcgcggc attctttgag ggacccccat 601 tcaagttcaa gttcacagtt ggcgaccaca gccgctttgt caactgtgtg cgattctctc 661 ctgatgggaa cagatttgcc acagccagtg ctgacggcca gatatacatc tatgacggga 721 agactgggga gaaggtgtgc gcgctgggcg gaagcaaggc ccacgacggt gggatttacg 781 caattagttg gagtcccgac agcacccatt tgctttctgc ttctggggac aaaacttcca 841 agatttggga cgtcagcgtg aactccgtgg tcagcacatt tcccatgggc tccacggttc 901 tggaccagca gctgggctgc ctatggcaga aggaccacct gctcagtgtc tccctgtccg 961 ggtacatcaa ctatctggac agaaacaacc ccagcaagcc cctgcacgtc atcaagggtc 1021 acagtaaatc gatccagtgt ctgacggtgc ataaaaacgg cggcaagtcc tacatttact 1081 ctgggagcca cgacggacac attaattact gggattcaga gacgggggag aacgactcct 1141 tcgctgggaa aggccacacg aaccaggtgt ccaggatgac cgtggatgag tcggggcagc 1201 tcatcagctg cagcatggac gacaccgtgc ggtacaccag cctcatgctg cgggactaca 1261 gcggacaagg agttgtgaaa ctggacgttc agccaaagtg cgtagccgtc ggccccgggg 1321 gatacgccgt ggtcgtgtgc attggacaga ttgtcctgct gaaggatcag aggaagtgct 1381 tcagcatcga caaccccggc tacgagcccg aagttgtggc agtgcacccc ggcggggaca 1441 cggtggcaat tgggggtgtg gacggcaacg tccgcctgta ttccatcctg ggcaccacgc 1501 tgaaggatga gggcaagctc ctagaggcca agggccccgt gaccgacgtg gcctactccc 1561 acgacggcgc cttcctcgcg gtgtgcgacg ccagcaaggt ggtcacagtg ttcagcgttg 1621 ctgacggcta ctcggagaac aatgtttttt atggacacca tgcaaaaatc gtctgcctgg 1681 cctggtcccc agacaatgaa cactttgcct ccggtggcat ggacatgatg gtgtatgttt 1741 ggaccctgag tgacccggaa accagagtca agatccaaga tgcacaccgg ctgcaccatg 1801 tcagcagcct ggcctggctg gacgagcaca cgctggtcac gacctcccat gatgcctctg 1861 tcaaggagtg gacaatcacc tactgcccaa ctttcttgta caaagtggtt ggtaagccta 1921 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1981 gcgttaagtc gacaatcaac ctctggatta