Construct: ORF ccsbBroad304_07687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010968.1_s304c1, BRDN0000400180
- Derived from:
- ccsbBroadEn_07687
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRS2 (10818)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07687
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10818 | FRS2 | fibroblast growth factor re... | NM_001042555.3 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 2 | human | 10818 | FRS2 | fibroblast growth factor re... | NM_001278351.2 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 3 | human | 10818 | FRS2 | fibroblast growth factor re... | NM_001278353.2 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 4 | human | 10818 | FRS2 | fibroblast growth factor re... | NM_001278354.2 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 5 | human | 10818 | FRS2 | fibroblast growth factor re... | NM_001278355.2 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 6 | human | 10818 | FRS2 | fibroblast growth factor re... | NM_001278356.2 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 7 | human | 10818 | FRS2 | fibroblast growth factor re... | NM_001278357.2 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 8 | human | 10818 | FRS2 | fibroblast growth factor re... | NM_006654.5 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 9 | human | 10818 | FRS2 | fibroblast growth factor re... | XM_017018717.1 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 10 | human | 10818 | FRS2 | fibroblast growth factor re... | XM_017018718.1 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 11 | human | 10818 | FRS2 | fibroblast growth factor re... | XM_017018719.1 | 99.1% | 99.2% | 654G>A;1524_1525insTTAGCCTGGAAA |
| 12 | mouse | 327826 | Frs2 | fibroblast growth factor re... | NM_177798.3 | 89% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1602
- ORF length:
- 1536
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tagctgttgt agctgtccag ataaagacac tgtcccagat aaccatcgga 121 acaagtttaa ggtcattaat gtggatgatg atgggaatga gttaggttct ggcataatgg 181 aacttacaga cacagaactg attttataca cccgcaaacg tgactcagta aaatggcact 241 acctctgcct gcgacgctat ggctatgact cgaatctctt ttcttttgaa agtggtcgaa 301 ggtgtcaaac tggacaagga atctttgcct ttaagtgtgc ccgtgcagaa gaattattta 361 acatgttgca agagattatg caaaataata gtataaatgt ggtggaagag ccagttgtag 421 aaagaaataa tcatcagaca gaattggaag tccctagaac acctcgaaca cctacaactc 481 caggatttgc tgctcagaac ttacctaatg gatatccccg atatccctca tttggagatg 541 cttcatccca tccgtcaagc agacatcctt ctgtgggaag tgctcgcctg ccttcagtag 601 gggaagaatc tacacatcct ttgcttgtgg ctgaggaaca agtacatacc tatgtcaaca 661 ctacaggtgt gcaagaagag cggaaaaacc gcacaagtgt gcatgttcca ttggaggcaa 721 gggtttctaa cgctgaaagc agcacaccaa aagaagaacc aagtagtatt gaggacaggg 781 atcctcagat tcttcttgaa cctgaaggag tcaaatttgt tttagggcca acccctgttc 841 aaaagcagtt aatggaaaaa gagaaactgg agcaacttgg aagagatcaa gttagtggaa 901 gtggagcaaa taacacagaa tgggacactg gctatgacag tgatgaacga agagatgcac 961 cctctgttaa caaactggtG TATGAAAATA TAAATGGGCT ATCTATCCCT AGTGCCTCAG 1021 GGGTCAGGAG AGGTCGTCTG ACATCCACCA GTACCTCAGA TACCCAGAAT ATCAACAACT 1081 CAGCTCAGAG AAGAACTGCA TTATTAAACT ATGAAAATCT ACCATCTTTG CCTCCTGTTT 1141 GGGAAGCCCG CAAGCTAAGT AGGGATGAAG ATGACAATTT AGGACCAAAG ACCCCATCTC 1201 TAAATGGCTA CCATAATAAT CTAGATCCAA TGCATAACTA TGTAAATACA GAGAATGTAA 1261 CAGTGCCAGC AAGTGCTCAC AAAATAGAAT ATTCAAGGCG TCGGGACTGT ACACCAACAG 1321 TCTTTAACTT TGATATCAGA CGCCCAAGTT TAGAACACAG GCAGCTTAAT TACATACAGG 1381 TTGACTTGGA AGGTGGCAGT GACTCTGACA ACCCTCAGAC TCCAAAAACG CCTACAACTC 1441 CCCTTCCACA AACCCCTACC AGGCGCACAG AGCTGTATGC CGTGATAGAC ATCGAGAGAA 1501 CTGCTGCTAT GTCAAATTTG CAGAAAGCAC TGCCACGAGA TGATGGTACA TCTAGGAAAA 1561 CTAGACACAA TAGTACTGAT CTGCCCATGT TAGCCTGGAA ATGCCCAACT TTCTTGTACa 1621 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1681 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta