Construct: ORF ccsbBroad304_07701
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006736.1_s304c1, BRDN0000400024
- Derived from:
- ccsbBroadEn_07701
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RUNDC3A (10900)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07701
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10900 | RUNDC3A | RUN domain containing 3A | NM_006695.4 | 99.9% | 100% | 729T>C |
| 2 | human | 10900 | RUNDC3A | RUN domain containing 3A | XM_017024040.2 | 98.9% | 99% | 221_222insAGCCTGTGCCCC;717T>C |
| 3 | human | 10900 | RUNDC3A | RUN domain containing 3A | NM_001144826.1 | 98.6% | 98.7% | 221_222insAGCCTGTGCCCCAGC;714T>C |
| 4 | human | 10900 | RUNDC3A | RUN domain containing 3A | XM_017024039.1 | 93.4% | 93.5% | 729T>C;1198_1281del |
| 5 | human | 10900 | RUNDC3A | RUN domain containing 3A | XM_017024038.2 | 93.2% | 93.3% | 729T>C;1198_1284del |
| 6 | human | 10900 | RUNDC3A | RUN domain containing 3A | NM_001144825.2 | 90% | 89.7% | (many diffs) |
| 7 | human | 10900 | RUNDC3A | RUN domain containing 3A | XM_024450548.1 | 89.1% | 88.8% | (many diffs) |
| 8 | human | 10900 | RUNDC3A | RUN domain containing 3A | XM_005256964.3 | 88.8% | 88.5% | (many diffs) |
| 9 | human | 10900 | RUNDC3A | RUN domain containing 3A | XM_017024035.1 | 85% | 84.5% | (many diffs) |
| 10 | human | 10900 | RUNDC3A | RUN domain containing 3A | XM_017024036.2 | 84.1% | 83.7% | (many diffs) |
| 11 | human | 10900 | RUNDC3A | RUN domain containing 3A | XM_017024037.1 | 83.9% | 83.5% | (many diffs) |
| 12 | human | 10900 | RUNDC3A | RUN domain containing 3A | XR_001752409.2 | 49% | 1_250del;979T>C;1466_2477del | |
| 13 | human | 10900 | RUNDC3A | RUN domain containing 3A | XR_001752408.2 | 47.3% | (many diffs) | |
| 14 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_006533693.2 | 90.5% | 96% | (many diffs) |
| 15 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_017314649.1 | 89.6% | 95% | (many diffs) |
| 16 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_006533694.2 | 89.3% | 94.8% | (many diffs) |
| 17 | mouse | 51799 | Rundc3a | RUN domain containing 3A | NM_001252347.1 | 88.7% | 94.1% | (many diffs) |
| 18 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_017314648.1 | 88.1% | 93.5% | (many diffs) |
| 19 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_011249124.1 | 86.4% | 91.7% | (many diffs) |
| 20 | mouse | 51799 | Rundc3a | RUN domain containing 3A | NM_016759.4 | 81.5% | 86.1% | (many diffs) |
| 21 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_017314647.1 | 80.7% | 85.2% | (many diffs) |
| 22 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_006533690.1 | 80.1% | 84.6% | (many diffs) |
| 23 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_006533689.1 | 79.6% | 84% | (many diffs) |
| 24 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_006533691.1 | 79.3% | 83.7% | (many diffs) |
| 25 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_006533692.1 | 79.1% | 83.5% | (many diffs) |
| 26 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XM_006533688.1 | 78.2% | 82.6% | (many diffs) |
| 27 | mouse | 51799 | Rundc3a | RUN domain containing 3A | XR_001780024.1 | 52.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1281
- ORF length:
- 1215
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agcgagcttt gtccagacca ccatggctct ggggctgtcc tccaagaaag 121 cgtcctctcg caacgtggct gtggagcgta agaacctgat caccgtgtgc aggttctctg 181 tgaaaacgct gctggagaag tacacagcgg agcccatcga tgactcatcg gaggagtttg 241 tcaattttgc agccatttta gagcagatcc tcagccaccg cttcaaagcc tgtgccccag 301 caggtccagt gagctggttc agctcagacg ggcagcgggg cttttgggac tatatccggc 361 tggcctgcag caaagtgccc aacaactgtg tgagcagcat cgagaacatg gagaacatca 421 gcacagcccg ggccaagggc cgggcatgga tccgggtggc actgatggag aagcgcatgt 481 cagaatacat caccacggct ctgcgtgaca cccggaccac cagacggttc tatgactctg 541 gagccatcat gctgcgggat gaagccacca tcctcaccgg aatgctgatc ggactgagcg 601 ccatcgactt cagcttctgt ctaaaggggg aagtcctgga cgggaagacc cccgtggtca 661 tcgattacac gccctaccta aagttcacgc agagctacga ctacctgacg gacgaggagg 721 agcggcacag cgccgagagc agcacgagcg aggacaactc gcccgagcac ccgtacctcc 781 cgctcgtcac cgacgaggac agctggtaca gcaagtggca caagatggag cagaagttcc 841 gcatcgtcta cgcgcagaag ggctacctgg aggagctggt gcgtctgcgc gagtcgcagc 901 tgaaggacct ggaggcggag aaccggcggc ttcagctgca gctggaggag gcggcggcgc 961 agaaccagcg cgagaaacgg gagctggaag gcgtgatcct ggagctgcag gagcagctga 1021 caggtctgat ccccagtgac cacgcccctc tggcccaggg ttccaaggag ctcactacac 1081 ccctggtcaa tcaatggccc tcactgggaa cgcttaatgg ggccgagggc gccagcaact 1141 ccaagctcta ccggagacac agcttcatga gcacggagcc gctgtcagct gaagccagtc 1201 tgagctcgga ctcccagcgc ctgggagagg gcacgcggga cgaggagccc tggggtccca 1261 tcggaagctc agagccaaat tacccaactt tcttgtacaa agtggttggt aagcctatcc 1321 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1381 ttaagtcgac aatcaacctc tggatta