Construct: ORF ccsbBroad304_07845
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006142.1_s304c1, BRDN0000462325
- Derived from:
- ccsbBroadEn_07845
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D9 (23158)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07845
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23158 | TBC1D9 | TBC1 domain family member 9 | NM_015130.3 | 99.9% | 100% | 3153C>T |
| 2 | mouse | 71310 | Tbc1d9 | TBC1 domain family, member 9 | NM_001111304.1 | 86.8% | 95.8% | (many diffs) |
| 3 | mouse | 71310 | Tbc1d9 | TBC1 domain family, member 9 | NM_027758.4 | 70.8% | 77.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3867
- ORF length:
- 3798
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgggtgaac ccggaggagg tgttgctggc caacgcgctg tggatcaccg 121 agagggccaa cccatacttc atcctgcagc ggaggaaggg ccacgccggc gatggaggcg 181 gcggcggcgg actggcgggc ctgctggtgg gtacccttga tgttgtgttg gactccagcg 241 cccgggtcgc tccttaccga atcttgtacc agactccaga ctccctggtc tactggacca 301 tcgcctgtgg tggttccagg aaagaaatca ctgaacactg ggaatggctt gagcaaaatc 361 tcttgcagac actctccatc tttgaaaatg agaatgatat caccacattt gtgagaggaa 421 aaatacaggg catcattgca gaatacaaca aaatcaatga tgtaaaggaa gatgatgaca 481 cggagaagtt taaagaagcc attgtgaaat ttcataggct gtttgggatg ccagaggaag 541 agaaactcgt caactattac tcttgcagct attggaaggg gaaggtcccc cgtcagggtt 601 ggatgtacct cagcattaac cacctttgct tttattcttt tcttatggga agggaagcga 661 aactggtcat ccggtgggta gacatcactc agcttgagaa gaatgccacc ctgcttctgc 721 ctgatgtgat caaagtgagc acacggtcca gtgagcattt cttctctgta ttcctcaaca 781 tcaacgagac cttcaagtta atggagcagc ttgccaacat agccatgagg caactcttag 841 acaatgaggg atttgaacaa gatcgatccc tgcccaaact caaaaggaaa tctcctaaaa 901 aagtgtctgc tctaaaacgt gatcttgatg ccagggcaaa gagtgagaga taccgtgcac 961 ttttccggct gcccaaagat gaaaaattag atggccacac agactgcact ctctggactc 1021 catttaacaa aatgcacatt ttggggcaga tgtttgtgtc cacaaattac atctgtttta 1081 ccagcaagga ggagaactta tgtagcctca ttatcccgct ccgtgaggtg acaattgtgg 1141 aaaaggcaga cagctccagt gtgctcccca gtcccttatc catcagcacc cgaaacagga 1201 tgaccttcct atttgccaac ttgaaagata gagactttct agtgcagagg atctcagatt 1261 tcctgcaaca gactacttcc aaaatatatt ctgacaagga gtttgcagga agttacaaca 1321 gttcagatga tgaggtgtac tctcgaccca gcagcctcgt ctcctccagc ccccagagaa 1381 gcacgagctc tgatgctgat ggagagcgcc agtttaacct aaatggcaac agcgtcccca 1441 cagccacaca gaccctgatg accatgtatc ggcggcggtc tcccgaggag ttcaacccga 1501 aattggccaa agagtttctg aaagagcaag cctggaagat tcactttgct gagtatgggc 1561 aagggatctg catgtaccgc acagagaaaa cgcgggagct ggtgttgaag ggcatcccgg 1621 agagcatgcg tggggagctc tggctgctgc tgtcaggtgc catcaatgag aaggccacac 1681 atcctgggta ctatgaagac ctagtggaga agtccatggg gaagtataat ctcgccacgg 1741 aggagattga gagggattta caccgctccc ttccagaaca cccagctttt cagaatgaaa 1801 tgggcattgc tgcactaagg agagtcttaa cagcttatgc ttttcgaaat cccaacatag 1861 ggtattgcca ggccatgaat attgtcactt cagtgctgct gctttatgcc aaagaggagg 1921 aagctttctg gctgcttgtg gctttgtgtg agcgcatgct cccagattac tacaacacca 1981 gagttgtggg tgcactggtg gaccaaggtg tctttgagga gctagcacga gactacgtcc 2041 cacagctgta cgactgcatg caagacctgg gcgtgatttc caccatctcc ctgtcttggt 2101 tcctcacact atttctcagt gtgatgcctt ttgagagtgc agttgtggtt gttgactgtt 2161 tcttctatga aggaattaaa gtgatattcc agttggccct agctgtgctg gatgcaaatg 2221 tggacaaact gttgaactgc aaggatgatg gggaggccat gaccgttttg ggaaggtatt 2281 tagacagtgt gaccaataaa gacagcacac tgcctcccat tcctcacctc cactccttgc 2341 tcagcgatga tgtggaacct taccctgagg tagacatctt tagactcatc agaacttcct 2401 acgagaaatt cggaactatc cgggcagatt tgattgaaca gatgagattc aaacagagac 2461 tgaaagtgat ccagacgctg gaggatacta cgaaacgcaa cgtggtacga accattgtga 2521 cagaaacttc ctttaccatt gatgagctgg aagaacttta tgctcttttc aaggcagaac 2581 atctcaccag ctgctactgg ggcgggagca gcaacgcgct ggaccggcat gaccccagcc 2641 tgccctacct ggaacagtat cgcattgact tcgagcagtt caagggaatg tttgctcttc 2701 tctttccttg ggcatgtgga actcactctg acgttctggc ctcccgcttg ttccagttat 2761 tagatgaaaa tggagactct ttgattaact tccgggagtt tgtctctggg ctaagtgctg 2821 catgccatgg ggacctcaca gagaagctca aactcctgta caaaatgcac gtcttgcctg 2881 agccatcctc tgatcaagat gaaccagatt ctgcttttga agcaactcag tacttctttg 2941 aagatattac cccagaatgt acacatgttg ttggattgga tagcagaagc aaacagggtg 3001 cagatgatgg ctttgttacg gtgagcctaa agccagacaa agggaagaga gcaaattccc 3061 aagaaaatcg taattatttg agactgtgga ctccagaaaa taaatctaag tcaaagaatg 3121 caaaggattt acccaaatta aatcaggggc agttcattga actgtgtaag acaatgtata 3181 acatgttcag cgaagacccc aatgagcagg agctgtacca tgccacggca gcagtgacca 3241 gcctcctgct ggagattggg gaggtcggca agttgttcgt ggcccagcct gcaaaggagg 3301 gcgggagcgg aggcagtggg ccgtcctgcc accagggcat cccaggcgtg ctcttcccca 3361 agaaagggcc aggccagcct tacgtggtgg agtctgttga gcccctgccg gccagcctgg 3421 cccccgacag cgaggaacac tcccttggag gacaaatgga ggacatcaag ctggaggact 3481 cctcgccccg ggacaacggg gcctgctcct ccatgctgat ctctgacgac gacaccaagg 3541 acgacagctc catgtcctca tactcggtgc tgagtgccgg ctcccacgag gaggacaagc 3601 tgcactgcga ggacatcgga gaggacacgg tcctggtgcg gagcggccag ggcacggcgg 3661 cactgccccg gagcaccagc ctggaccggg actgggccat caccttcgag cagttcctgg 3721 cctccctctt aactgagcct gccctggtca agtactttga caagcccgtg tgcatgatgg 3781 ccaggattac cagtgcaaaa aacatccgga tgatgggcaa gcccctcacc tcggccagtg 3841 actatgaaat ctcggccatg tccggcttgc caactttctt gtacaaagtg gttggtaagc 3901 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 3961 ggcgcgttaa gtcgacaatc aacctctgga tta