Construct: ORF ccsbBroad304_07866
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015398.1_s304c1, BRDN0000461960
- Derived from:
- ccsbBroadEn_07866
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC9A8 (23315)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07866
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | NM_015266.3 | 99.9% | 100% | 975T>G |
| 2 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | NM_001260491.1 | 97.2% | 97.3% | 532_579del;1023T>G |
| 3 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_006723756.1 | 94.5% | 94.4% | 714T>C;719_817del;1074T>G |
| 4 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528739.2 | 92.1% | 90.7% | (many diffs) |
| 5 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528736.2 | 92.1% | 92% | (many diffs) |
| 6 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528741.2 | 90.7% | 90.7% | 532_579del;1005_1006ins117 |
| 7 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528737.1 | 87.6% | 87.6% | (many diffs) |
| 8 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528738.2 | 85.9% | 85.8% | (many diffs) |
| 9 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528740.2 | 80.8% | 78.6% | (many diffs) |
| 10 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_017027754.2 | 80.1% | 78.9% | (many diffs) |
| 11 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528742.1 | 58.8% | 58.7% | (many diffs) |
| 12 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_017027755.2 | 58.8% | 58.7% | (many diffs) |
| 13 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528743.2 | 57.4% | 57.4% | 0_1ins741;234T>G |
| 14 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_017027756.2 | 57.4% | 57.4% | 0_1ins741;234T>G |
| 15 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528744.2 | 51.6% | 51.2% | (many diffs) |
| 16 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | XM_011528745.2 | 45% | 41.3% | (many diffs) |
| 17 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | NR_048537.2 | 27.7% | (many diffs) | |
| 18 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | NR_048538.2 | 26.4% | (many diffs) | |
| 19 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | NR_048539.2 | 25.6% | (many diffs) | |
| 20 | human | 23315 | SLC9A8 | solute carrier family 9 mem... | NR_048540.2 | 22.8% | (many diffs) | |
| 21 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | NM_148929.3 | 88% | 95.6% | (many diffs) |
| 22 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | NM_001304542.1 | 83.8% | 90.3% | (many diffs) |
| 23 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | XM_006500395.3 | 80.1% | 87.2% | (many diffs) |
| 24 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | NM_001304540.1 | 74.3% | 80.3% | (many diffs) |
| 25 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | XM_006500398.3 | 63.8% | 64% | (many diffs) |
| 26 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | XM_006500399.1 | 55.7% | 59.8% | (many diffs) |
| 27 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | XM_006500400.1 | 55.7% | 59.8% | (many diffs) |
| 28 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | XR_001783205.1 | 31.6% | (many diffs) | |
| 29 | mouse | 77031 | Slc9a8 | solute carrier family 9 (so... | NR_130767.1 | 29% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1812
- ORF length:
- 1743
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggagaag atggcggaag aggagaggtt ccccaataca actcatgagg 121 gtttcaatgt caccctccac accaccctgg ttgtcacgac gaaactggtg ctcccgaccc 181 ctggcaagcc catcctcccc gtgcagacag gggagcaggc ccagcaagag gagcagtcca 241 gcggcatgac cattttcttc agcctccttg tcctagctat ctgcatcata ttggtgcatt 301 tactgatccg atacagatta catttcttgc cagagagtgt tgctgttgtt tctttaggta 361 ttctcatggg agcagttata aaaattatag agtttaaaaa actggcgaat tggaaggaag 421 aagaaatgtt tcgtccaaac atgtttttcc tcctcctgct tccccctatt atctttgagt 481 ctggatattc attacacaag ggtaacttct ttcaaaatat tggttccatc accctgtttg 541 ctgtttttgg gacggcaatc tccgcttttg tagtaggtgg aggaatttat tttctgggtc 601 aggctgatgt aatctctaaa ctcaacatga cagacagttt tgcgtttggc tccctaatat 661 ctgctgtcga tccagtggcc actattgcca ttttcaatgc acttcatgtg gaccccgtgc 721 tcaacatgct ggtctttgga gaaagtattc tcaacgatgc agtctccatt gttctgacca 781 acacagctga aggtttaaca agaaaaaata tgtcagatgt cagtgggtgg caaacatttt 841 tacaagccct tgactacttc ctcaaaatgt tctttggctc tgcagcgctc ggcactctca 901 ctggcttaat ttctgcatta gtgctgaagc atattgactt gaggaaaacg ccttccttgg 961 agtttggcat gatgatcatt tttgcttatc tgccttatgg gcttgcagaa ggaatctcac 1021 tctcaggcat catggccatc ctgttctcag gcatcgtgat gtcccactac acgcaccata 1081 acctctcccc agtcacccag atcctcatgc agcagaccct ccgcaccgtg gccttcttat 1141 gtgaaacatg tgtgtttgca tttcttggcc tgtccatttt tagttttcct cacaagtttg 1201 aaatttcctt tgtcatctgg tgcatagtgc ttgtactatt tggcagagcg gtaaacattt 1261 tccctctttc ctacctcctg aatttcttcc gggatcataa aatcacaccg aagatgatgt 1321 tcatcatgtg gtttagtggc ctgcggggag ccatccccta tgccctgagc ctacacctgg 1381 acctggagcc catggagaag cggcagctca tcggcaccac caccatcgtc atcgtgctct 1441 tcaccatcct gctgctgggc ggcagcacca tgcccctcat tcgcctcatg gacatcgagg 1501 acgccaaggc acaccgcagg aacaagaagg acgtcaacct cagcaagact gagaagatgg 1561 gcaacactgt ggagtcggag cacctgtcgg agctcacgga ggaggagtac gaggcccact 1621 acatcaggcg gcaggacctt aagggcttcg tgtggctgga cgccaagtac ctgaacccct 1681 tcttcactcg gaggctgacg caggaggacc tgcaccacgg gcgcatccag atgaaaactc 1741 tcaccaacaa gtggtacgag gaggtacgcc agggcccctc cggctccgag gacgacgagc 1801 aggagctgct cttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1861 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1921 caatcaacct ctggatta