Construct: ORF ccsbBroad304_07867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010627.1_s304c1, BRDN0000401904
- Derived from:
- ccsbBroadEn_07867
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUT4 (23318)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07867
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23318 | TUT4 | terminal uridylyl transfera... | NM_001009881.3 | 99.9% | 100% | 315G>A |
| 2 | human | 23318 | TUT4 | terminal uridylyl transfera... | NM_015269.2 | 99.9% | 99.9% | 315G>A;3936_3937insAGC |
| 3 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_005270680.3 | 99.6% | 99.6% | 315G>A;3936_3937insAGCAGTTTACTGTTT |
| 4 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_005270676.2 | 98.6% | 97.7% | (many diffs) |
| 5 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_006710498.2 | 98.6% | 97.7% | (many diffs) |
| 6 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_006710499.3 | 98.6% | 97.7% | (many diffs) |
| 7 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_011541097.1 | 98.6% | 97.7% | (many diffs) |
| 8 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_005270678.2 | 98.5% | 97.7% | (many diffs) |
| 9 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_005270679.2 | 98.3% | 97.4% | (many diffs) |
| 10 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000804.2 | 98% | 98% | 315G>A;1513_1514ins81;3855_3856insAGCAGTTTACTGTTT |
| 11 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000805.2 | 97.3% | 97.3% | 315G>A;3319_3320ins114;3822_3823insAGCAGTTTACTGTTT |
| 12 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_011541098.1 | 96.9% | 96.1% | (many diffs) |
| 13 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000803.1 | 96.6% | 95.8% | (many diffs) |
| 14 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_005270681.2 | 96.3% | 95.4% | (many diffs) |
| 15 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000807.2 | 95.3% | 95.3% | 315G>A;1635_1636ins213;3723_3724insAGCAGTTTACTGTTT |
| 16 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_006710500.1 | 94.3% | 93.5% | (many diffs) |
| 17 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000806.1 | 94% | 93.2% | (many diffs) |
| 18 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000809.2 | 93.2% | 93.2% | 315G>A;1515_1516ins333 |
| 19 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000810.2 | 92.9% | 92.9% | 315G>A;1515_1516ins333;3603_3604insAGCAGTTTACTGTTT |
| 20 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_011541099.1 | 92.7% | 91.8% | (many diffs) |
| 21 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000811.2 | 92.5% | 92.5% | 315G>A;3065_3066ins366 |
| 22 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000812.2 | 92.5% | 92.5% | 315G>A;3065_3066ins366;3570_3571insAGC |
| 23 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000813.2 | 92.2% | 92.2% | 315G>A;3065_3066ins366;3570_3571insAGCAGTTTACTGTTT |
| 24 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_005270682.2 | 91.9% | 91.1% | (many diffs) |
| 25 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000808.1 | 91.6% | 90.7% | (many diffs) |
| 26 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_005270683.2 | 91.2% | 90.4% | (many diffs) |
| 27 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000814.2 | 85.8% | 85.8% | 315G>A;1515_1516ins333;2732_2733ins366 |
| 28 | human | 23318 | TUT4 | terminal uridylyl transfera... | XR_001737073.1 | 81.5% | (many diffs) | |
| 29 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_011541100.2 | 78.2% | 77% | (many diffs) |
| 30 | human | 23318 | TUT4 | terminal uridylyl transfera... | XR_001737074.2 | 74.4% | (many diffs) | |
| 31 | human | 23318 | TUT4 | terminal uridylyl transfera... | XR_002959846.1 | 67% | (many diffs) | |
| 32 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_011541101.2 | 62.8% | 60.6% | (many diffs) |
| 33 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000815.2 | 62.4% | 60.2% | (many diffs) |
| 34 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000816.1 | 61.8% | 60.7% | (many diffs) |
| 35 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000817.1 | 59.1% | 58% | (many diffs) |
| 36 | human | 23318 | TUT4 | terminal uridylyl transfera... | XM_017000818.1 | 56.7% | 55.6% | (many diffs) |
| 37 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_017320139.2 | 87% | 86.6% | (many diffs) |
| 38 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_006502978.4 | 87% | 86.5% | (many diffs) |
| 39 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_006502980.3 | 87% | 86.5% | (many diffs) |
| 40 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_017320138.2 | 86.9% | 86.5% | (many diffs) |
| 41 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_017320140.2 | 86.7% | 86.3% | (many diffs) |
| 42 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | NM_175472.3 | 86.7% | 86.2% | (many diffs) |
| 43 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_030253437.1 | 86.7% | 86.2% | (many diffs) |
| 44 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_017320141.2 | 86% | 85.5% | (many diffs) |
| 45 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_006502983.4 | 54.2% | 51.7% | (many diffs) |
| 46 | mouse | 230594 | Tut4 | terminal uridylyl transfera... | XM_006502984.4 | 47.8% | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5004
- ORF length:
- 4935
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagagtct aaaaccttaa aaagtgaaaa tcatgaacca aagaagaatg 121 ttatttgtga agaaagcaaa gcagttcaag ttataggtaa tcaaacattg aaagctagaa 181 atgataaatc cgtaaaagaa attgagaaca gctctccaaa taggaatagt agtaaaaaaa 241 ataagcaaaa tgatatttgt atagaaaaaa cagaagttaa atcatgtaaa gtaaatgctg 301 ccaaccttcc aggtcctaaa gatttggggt tagtccttcg agatcaaagt cattgcaaag 361 caaaaaaatt tcctaattca ccagtgaaag ccgaaaaggc aaccatttca caggcaaaat 421 cagaaaaggc aaccagttta caggcaaaag cagaaaaatc accaaagtca cctaattcag 481 tgaaagcaga aaaagcatcc agttatcaga tgaagtcaga aaaagtacca agttcaccag 541 cagaagcaga aaagggaccc agtttactgt tgaaagacat gagacagaag acagaattac 601 aacagattgg aaaaaaaatt ccaagctcct ttacttctgt ggacaaagtg aatattgaag 661 ctgtaggggg agaaaaatgt gctctgcaaa actcaccacg atctcagaag caacagacat 721 gtacagataa tactggtgat tctgatgata gtgcttcagg aattgaagac gtatcggacg 781 atttaagtaa aatgaagaat gatgaatcta ataaagaaaa ttcttcagag atggactact 841 tagaaaatgc cactgtgata gatgaatctg cattgacacc tgagcagagg ctggggttga 901 aacaagcaga agaacgctta gaaagagatc acatctttcg acttgaaaaa cgatcaccag 961 aatataccaa ttgtcggtat ctatgcaaac tttgcttaat tcacattgaa aatatccagg 1021 gggctcataa acatataaag gagaaacgac ataagaaaaa tattttggaa aaacaagaag 1081 aaagtgagct tcgttctctg ccacctcctt cccctgccca cttggctgct ttaagtgttg 1141 cagtcattga attagcaaaa gaacatggaa taacagatga tgacctcaga gtccgtcagg 1201 aaattgtgga ggaaatgtca aaggttataa cgacattttt accagaatgt tcacttaggt 1261 tgtatggctc atctctgact agatttgctc tgaaaagtag tgatgttaat atagatataa 1321 aatttcctcc caagatgaat catccagatc ttctgataaa agtacttggg attttaaaga 1381 aaaatgtatt atatgtagat gtggaatctg attttcacgc taaagttcct gttgtggtgt 1441 gcagagatcg aaaaagtggt ttactttgta gagtgagtgc aggaaacgat atggcatgtc 1501 tcactactga tttacttact gcccttggca aaatagaacc tgtctttatt cccttggtgt 1561 tagcctttcg ctactgggct aagttgtgct atattgactc ccaaactgat ggtggaatcc 1621 cttcttactg ttttgcttta atggtgatgt tttttctaca acagagaaaa ccccctcttc 1681 ttccttgctt acttggaagt tggattgaag gctttgaccc aaaaagaatg gatgactttc 1741 agctgaaggg catagtagaa gagaagtttg tgaagtggga atgtaattca agtagtgcaa 1801 ctgagaaaaa ctcaattgct gaggaaaaca aagctaaggc agaccaacca aaagatgata 1861 ccaagaagac agaaacagac aaccaaagta atgccatgaa ggaaaaacat ggcaaatctc 1921 ctttagcatt ggaaacacca aatcgggtat ccttgggaca gttatggtta gagctgctta 1981 aattctacac actggatttt gctttggagg aatatgtcat atgtgtacgg atacaagata 2041 ttttaacaag agaaaataaa aactggccta aaaggcgaat agccattgaa gatccatttt 2101 cagtcaagag gaatgttgca cggagcttaa acagccagct ggtttacgaa tatgttgtgg 2161 agagatttag ggcagcttat cggtattttg cctgtcctca gacgaagggt ggaaataagt 2221 ctacagtgga tttcaagaaa agagagaagg ggaaaataag caataagaaa ccagtcaagt 2281 cgaacaatat ggcaaccaat ggttgtattc tgcttgggga aaccacagaa aaaataaatg 2341 cagaaagaga gcaacctgtt caatgtgatg aaatggactg tacatcacag agatgtatta 2401 ttgacaacaa caatttgttg gtaaatgaac tagattttgc tgaccacgga caggactctt 2461 catctctttc taccagcaaa agcagtgaaa tagagccaaa attagataag aaacaagatg 2521 atttagcgcc ttcagaaact tgtttaaaaa aagagctcag ccaatgtaat tgcattgatt 2581 tgtctaagtc gcctgaccca gataaatcta ctggaacaga ctgcaggtca aatttagaaa 2641 cagagagttc acatcagagt gtgtgcaccg acacatctgc tacctcttgc aactgcaaag 2701 ctacagaaga tgcttctgac cttaatgatg atgataacct ccccacccag gaattatatt 2761 atgtgtttga taagtttatt ttaacctctg gcaagccacc aacgatagta tgcagcatct 2821 gcaaaaagga tggccattca aagaatgatt gcccagagga ttttaggaaa attgatttaa 2881 aacctctacc accaatgaca aaccgatttc gggaaatact tgatttagta tgtaaaagat 2941 gttttgatga gttatcacca ccttgttctg aacaacacaa cagggagcaa attttaattg 3001 gcttggaaaa gtttattcaa aaagaatatg atgaaaaggc aaggttgtgc ttatttggct 3061 cttctaagaa tggatttgga tttcgtgata gtgatctgga tatttgtatg accctggaag 3121 gccatgaaaa tgcagagaaa ttaaattgta aggaaataat tgaaaatttg gcaaaaattc 3181 ttaagagaca tccaggttta agaaacattt tgcctataac tactgccaaa gtgcctatag 3241 taaaatttga acacaggcga agtgggttag aaggcgatat cagtttatat aatacgttgg 3301 ctcaacataa cacaagaatg ctagctactt atgcagctat tgatcctaga gtgcagtatt 3361 tgggatatac tatgaaagtg tttgctaagc gatgtgacat tggggatgct tccaggggaa 3421 gtttatcttc atatgcatat atccttatgg tgctgtactt tctgcagcag agaaagccac 3481 ctgttatccc agttctacaa gagatctttg atggaaaaca gattccacag agaatggttg 3541 atggatggaa tgctttcttc tttgataaaa cagaagaact gaaaaagcgt ttaccttcac 3601 ttggaaagaa cacagaatca ttaggggagc tttggctggg actgcttcgt ttctatactg 3661 aagagtttga tttcaaggaa tatgtaatta gcattcggca gaaaaagctg ttgacaactt 3721 ttgagaagca gtggacttcc aagtgcattg caattgaaga cccttttgac ttgaatcata 3781 accttggtgc tggagtttcc agaaaaatga ccaatttcat catgaaagca tttatcaatg 3841 gaaggaaact ttttggtacc cctttttatc cactcattgg cagagaagct gagtacttct 3901 ttgattccag agtattaaca gatggagaac tggctcccaa tgatagatgt tgccgtgtgt 3961 gtggaaaaat aggccactac atgaaagact gccctaaaag gaaaagcagt ttactgttta 4021 gactaaagaa gaaagacagt gaagaagaga aggaagggaa tgaagaagag aaagattccc 4081 gagatgttct tgacccccga gacctccacg atactcgaga ctttagagac ccgagagacc 4141 tcagatgttt tatatgtgga gatgctggac atgtacgaag ggagtgccca gaggtcaagc 4201 tggcccgtca gaggaatagc agtgtggcag cagcccagct ggtccgcaac cttgtaaatg 4261 ctcaacaggt ggctggttca gctcagcaac agggtgatca gtccataagg actagacagt 4321 catcagaatg ttctgaatca ccatcttatt ctcctcagcc tcagccattt ccacagaact 4381 cttcccagtc agctgctatt actcagcctt catctcagcc aggatcccaa cctaagcttg 4441 gcccacctca gcagggagcc caacctcccc atcaggtcca gatgccactg tataactttc 4501 cccagtcacc accagctcag tattctccca tgcacaatat gggattgttg ccaatgcacc 4561 ctctccagat ccctgccccg tcctggccca tccatggccc agtgatccac tctgcaccag 4621 gcagtgcccc cagcaatatt ggcctaaatg atcccagcat catctttgca cagcctgctg 4681 ccagacctgt ggcaatccct aacacgtctc acgatggaca ctggccccgt actgtggctc 4741 caaattccct ggtaaacagt ggtgcagtgg ggaattcaga gccaggcttt cgaggactga 4801 ctcctccaat tccttgggaa catgcaccgc gtccccattt cccccttgtc ccagcttcgt 4861 ggccttatgg tttgcatcaa aacttcatgc atcagggaaa tgcccgattc cagcccaaca 4921 aacctttcta tactcaagac agatgtgcca cccgtcggtg tagagagcgt tgtccccacc 4981 caccaagagg aaacgtgtcg gagttgccaa ctttcttgta caaagtggtt ggtaagccta 5041 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 5101 gcgttaagtc gacaatcaac ctctggatta