Construct: ORF ccsbBroad304_07872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007193.1_s304c1, BRDN0000410274
- Derived from:
- ccsbBroadEn_07872
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRTC1 (23373)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07872
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23373 | CRTC1 | CREB regulated transcriptio... | NM_015321.3 | 99.9% | 99.8% | 982A>G |
| 2 | human | 23373 | CRTC1 | CREB regulated transcriptio... | NM_001098482.2 | 97.4% | 97.3% | 243_290del;1030A>G |
| 3 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_005259834.1 | 95.3% | 95.2% | 982A>G;1425_1426ins87 |
| 4 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_005259833.3 | 93% | 92.9% | 243_290del;1030A>G;1473_1474ins87 |
| 5 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_011527842.3 | 92.1% | 92% | 243_290del;1030A>G;1368_1369ins105 |
| 6 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_017026536.1 | 87% | 86.9% | (many diffs) |
| 7 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_005259835.3 | 84.2% | 84.1% | 243_290del;1030A>G;1056_1057ins258 |
| 8 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_005259836.3 | 78.8% | 78.7% | (many diffs) |
| 9 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_024451434.1 | 78.1% | 78% | 982A>G;1011_1012ins414 |
| 10 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_006722710.3 | 76.2% | 76.1% | 243_290del;1030A>G;1059_1060ins414 |
| 11 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_017026537.1 | 74.4% | 73.2% | (many diffs) |
| 12 | human | 23373 | CRTC1 | CREB regulated transcriptio... | XM_017026538.2 | 42.2% | 34.7% | (many diffs) |
| 13 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | NM_001004062.2 | 85% | 89.6% | (many diffs) |
| 14 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_011242311.2 | 81.5% | 85.9% | (many diffs) |
| 15 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_006509699.3 | 80.8% | 85.4% | (many diffs) |
| 16 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_006509700.3 | 79.6% | 84.1% | (many diffs) |
| 17 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_011242309.2 | 77.5% | 81.9% | (many diffs) |
| 18 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_011242310.2 | 76.4% | 80.7% | (many diffs) |
| 19 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_011242312.2 | 60.9% | 60.9% | (many diffs) |
| 20 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_011242313.2 | 60.1% | 62.8% | (many diffs) |
| 21 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_006509704.2 | 59% | 61.8% | (many diffs) |
| 22 | mouse | 382056 | Crtc1 | CREB regulated transcriptio... | XM_011242314.2 | 40.9% | 40.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1968
- ORF length:
- 1902
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gacttcgaac aatccgcgga aattcagcga gaagatcgcg ctgcacaatc 121 agaagcaggc ggaggagacg gcggccttcg aggaggtcat gaaggacctg agcctgacgc 181 gggccgcgcg gctccagctc cagaaatccc agtacctgca actgggcccc agccgaggcc 241 agtactatgg cgggtccctg cccaacgtga accagatcgg gagtggcacc atggacctgc 301 ccttccagac ccccttccaa tcctcgggcc tggacaccag ccggaccacc cggcaccatg 361 ggctggtgga cagggtgtac cgggagcgtg gccggctcgg ctccccacac cgccggcccc 421 tgtcagtgga caaacacgga cggcaggccg acagctgccc ctatggcacc atgtacctct 481 caccacccgc ggacaccagc tggagaagga ccaattctga ctccgccctg caccagagca 541 caatgacgcc cacgcagcca gaatccttta gcagtgggtc ccaggacgtg caccagaaaa 601 gagtcttact gttaacagtc ccaggaatgg aagagaccac atcagaggca gacaaaaacc 661 tttccaagca agcatgggac accaagaaga cggggtccag gcccaagtcc tgtgaggtcc 721 ccggaatcaa catcttcccg tctgccgacc aggaaaacac tacagccctg atccccgcca 781 cccacaacac aggggggtcc ctgcccgacc tgaccaacat ccacttcccc tccccgctcc 841 cgaccccgct ggaccccgag gagcccacct tccctgcact gagcagctcc agcagcaccg 901 gcaacctcgc ggccaacctg acgcacctgg gcatcggtgg cgccggccag ggaatgagca 961 cacctggctc ctctccacag caccgcccag ctggcgtcag ccccctgtcc ctgagcacag 1021 aggcaaggcg tcagcaggca tcgcccgccc tgtccccgct gtcacccatc actcaggctg 1081 tagccatgga cgccctgtct ctggagcagc agctgcccta cgccttcttc acccaggcgg 1141 gctcccagca gccaccgccg cagccccagc ccccgccgcc tcctccaccc gcgtcccagc 1201 agccaccacc cccgccaccc ccacaggcgc ccgtccgcct gccccctggt ggccccctgt 1261 tgcccagcgc cagcctgact cgtgggccac agccgccccc gcttgcagtc acggtaccgt 1321 cctctctccc ccagtccccc ccagagaacc ctggccagcc atcgatgggg atcgacatcg 1381 cctcggcgcc ggctctgcag cagtaccgca ctagcgccgg ctccccggcc aaccagtctc 1441 ccacctcgcc agtctccaat caaggcttct ccccagggag ctccccgcaa cacacttcca 1501 ccctgggcag cgtgtttggg gacgcgtact atgagcagca gatggcggcc aggcaggcca 1561 atgctctgtc ccaccagctg gagcagttca acatgatgga gaacgccatc agctccagca 1621 gcctgtacag cccgggctcc acactcaact actcgcaggc ggccatgatg ggcctcacgg 1681 gcagccacgg gagcctgccg gactcgcagc aactgggata cgccagccac agtggcatcc 1741 ccaacatcat cctcacagtg acaggagagt ccccccccag cctctctaaa gaactgacca 1801 gctctctggc cggggtcggc gacgtcagct tcgactccga cagccagttt cccctggacg 1861 aactcaagat cgaccccctg accctcgacg gactgcacat gctcaacgac cccgacatgg 1921 ttctggccga cccagccacc gaggacacct tccggatgga ccgcctgtgc ccaactttct 1981 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2041 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta