Transcript: Human NM_015321.3

Homo sapiens CREB regulated transcription coactivator 1 (CRTC1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
CRTC1 (23373)
Length:
6879
CDS:
24..1928

Additional Resources:

NCBI RefSeq record:
NM_015321.3
NBCI Gene record:
CRTC1 (23373)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_015321.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000437797 TTCGACTCCGACAGCCAGTTT pLKO_005 1788 CDS 100% 4.950 6.930 N CRTC1 n/a
2 TRCN0000419707 AGAGTCTTACTGTTAACAGTC pLKO_005 558 CDS 100% 4.050 2.835 N CRTC1 n/a
3 TRCN0000118894 CGCGTACTATGAGCAGCAGAT pLKO.1 1481 CDS 100% 4.050 2.835 N CRTC1 n/a
4 TRCN0000420691 GAATGGAAGAGACCACATCAG pLKO_005 583 CDS 100% 4.050 2.835 N CRTC1 n/a
5 TRCN0000175746 GCAGTTCAACATGATGGAGAA pLKO.1 1541 CDS 100% 4.050 2.835 N Crtc1 n/a
6 TRCN0000118892 CGAGCTTGTGATTCTGAGCTT pLKO.1 2016 3UTR 100% 2.640 1.848 N CRTC1 n/a
7 TRCN0000118893 GCCTCTCTAAAGAACTGACCA pLKO.1 1738 CDS 100% 2.640 1.848 N CRTC1 n/a
8 TRCN0000118895 GCTCCAGAAATCCCAGTACCT pLKO.1 155 CDS 100% 2.640 1.848 N CRTC1 n/a
9 TRCN0000118896 GCCAGTCTCCAATCAAGGCTT pLKO.1 1406 CDS 100% 2.640 1.584 N CRTC1 n/a
10 TRCN0000242281 CGGGCTCCACACTCAACTATT pLKO_005 1591 CDS 100% 13.200 9.240 N Crtc1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_015321.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07872 pDONR223 100% 99.9% 99.8% None 982A>G n/a
2 ccsbBroad304_07872 pLX_304 0% 99.9% 99.8% V5 982A>G n/a
3 TRCN0000467955 ACCTGTGTCCAGGGATGACCTATC pLX_317 7.7% 99.9% 99.8% V5 982A>G n/a
4 ccsbBroadEn_15010 pDONR223 56.1% 74.6% 74.4% None (many diffs) n/a
5 ccsbBroad304_15010 pLX_304 0% 74.6% 74.4% V5 (many diffs) n/a
6 TRCN0000474232 AGTACCACTGACGGGGACGCTCCT pLX_317 36.2% 74.6% 74.4% V5 (many diffs) n/a
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