Construct: ORF ccsbBroad304_07939

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF016295.1_s304c1, BRDN0000406920
• Derived from: ccsbBroadEn_07939
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: RASGRP3 (25780)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_07939
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_001139488.2	99.9%	99.8%	2062G>A
2	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_001349975.2	99.9%	99.8%	2062G>A
3	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_001349976.2	99.9%	99.8%	2062G>A
4	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_001349977.2	99.9%	99.8%	2062G>A
5	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_170672.3	99.9%	99.8%	2062G>A
6	human	25780	RASGRP3	RAS guanyl releasing protein 3	XM_011532746.3	99.9%	99.8%	2062G>A
7	human	25780	RASGRP3	RAS guanyl releasing protein 3	XM_011532748.3	99.9%	99.8%	2062G>A
8	human	25780	RASGRP3	RAS guanyl releasing protein 3	XM_017003759.2	99.9%	99.8%	2062G>A
9	human	25780	RASGRP3	RAS guanyl releasing protein 3	XM_017003761.2	99.9%	99.8%	2062G>A
10	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_001349978.2	99.8%	99.7%	1160_1161insGCA;2059G>A
11	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_001349979.2	99.8%	99.7%	1160_1161insGCA;2059G>A
12	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_001349980.2	99.8%	99.7%	1160_1161insGCA;2059G>A
13	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_001349981.2	99.8%	99.7%	1160_1161insGCA;2059G>A
14	human	25780	RASGRP3	RAS guanyl releasing protein 3	NM_015376.2	99.8%	99.7%	1160_1161insGCA;2059G>A
15	human	25780	RASGRP3	RAS guanyl releasing protein 3	XR_001738692.2	56.1%		(many diffs)
16	human	25780	RASGRP3	RAS guanyl releasing protein 3	XR_001738691.2	52%		(many diffs)
17	human	25780	RASGRP3	RAS guanyl releasing protein 3	XR_001738693.2	42.1%		(many diffs)
18	mouse	240168	Rasgrp3	RAS, guanyl releasing prote...	NM_001166493.1	89%	94%	(many diffs)
19	mouse	240168	Rasgrp3	RAS, guanyl releasing prote...	NM_207246.4	89%	94%	(many diffs)
20	mouse	240168	Rasgrp3	RAS, guanyl releasing prote...	XM_006524271.3	89%	94%	(many diffs)
21	mouse	240168	Rasgrp3	RAS, guanyl releasing prote...	XM_006524272.3	89%	94%	(many diffs)
22	mouse	240168	Rasgrp3	RAS, guanyl releasing prote...	XM_006524273.3	88.8%	93.9%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

2136

ORF length:

2070

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatggg atcaagtggc cttgggaaag cagcaacatt agatgaactg ctgtgcactt
121	gcattgagat gtttgatgac aatggagagc tggataatag ttatttgcca agaatagttc
181	tactgatgca ccgatggtat ttatcttcca ctgaattggc agaaaaactt ctctgcatgt
241	atcgaaatgc cactggagaa agctgcaatg aatttcgatt aaagatctgc tacttcatga
301	ggtactggat tctgaagttt cctgcagagt ttaatttgga tcttggtttg attcgtatga
361	ctgaggaatt tcgggaagta gctagtcaac taggatatga aaaacacgtc agcctcatcg
421	acatatccag cattccttcc tatgactgga tgagaagagt cacacagagg aaaaaagtat
481	ccaagaaggg aaaagcctgt ctgctgtttg accatctgga gcccattgaa ttggctgagc
541	acctcacttt tctggagcat aaatctttta gaaggatctc attcactgat taccaaagct
601	atgtcatcca tggctgcctg gagaataatc caaccttgga aagatcgatt gctttattta
661	atggaatctc taagtgggtc cagttgatgg ttcttagcaa accaaccccc cagcaaaggg
721	cagaagtcat cacaaagttt atcaatgttg caaagaagct ccttcagctc aaaaatttta
781	acaccctgat ggcagtggtg ggaggcctca gtcatagttc catttcacgc ctcaaagaga
841	cccattctca tctttcttca gaagttacaa agaactggaa tgaaatgaca gagttggtct
901	cctccaacgg caattactgc aattaccgca aggcctttgc cgactgcgat ggcttcaaaa
961	tccccatcct tggagtacac ttgaaagact tgatagctgt ccatgtcatt ttcccagact
1021	ggacagagga gaacaaagtg aacattgtga aaatgcacca gctctccgtt accctgagtg
1081	aactagtctc cctgcagaat gcctctcacc acttagaacc caacatggat ttgatcaacc
1141	tgctcacgct ttccctggac ctctatcaca ctgaagatga tatttacaaa ctgtcactgg
1201	tgctggagcc tagaaattct aaatcgcagc ctacctcccc tacgacgccc aacaagcctg
1261	tggtacccct ggagtgggca ttaggggtga tgccaaagcc agaccccacg gtcatcaaca
1321	agcacataag gaaattagtg gagtctgtat ttagaaacta tgatcacgac catgatgggt
1381	acatttccca agaggacttt gaaagtatag ctgccaattt tcccttcttg gattccttct
1441	gtgttctgga caaagatcag gatggcctaa ttagtaaaga tgaaatgatg gcttacttcc
1501	tgagagctaa atcccaacta cactgtaaaa tgggaccagg atttatccat aattttcagg
1561	agatgaccta tctcaagcca accttctgcg aacactgtgc gggatttctc tggggcataa
1621	tcaagcaagg atacaaatgc aaagactgtg gagccaattg tcacaaacag tgcaaagacc
1681	tcctggttct ggcctgcagg agatttgccc gggcgccctc cttgagcagt ggtcatgggt
1741	cactgcctgg aagcccctcg ctgcccccag cgcaggatga ggtgtttgag ttccctggag
1801	tcactgctgg acacagggat ttagacagca gagccatcac actggttaca ggctcttctc
1861	gcaagatctc tgtgaggcta cagagggcca ccaccagcca ggccacccag actgaacctg
1921	tctggtcaga ggctggctgg ggggactcgg ggtcccacac cttccctaaa atgaaatcca
1981	agttccatga caaagcagca aaggacaaag gctttgccaa atgggaaaat gagaagccca
2041	gggtgcatgc tggtgtggat gttgtagacc ggggcacgga gtttgaactt gaccaggatg
2101	aaggagaaga gaccagacag gatggtaagg atggctgccc aactttcttg tacaaagtgg
2161	ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
2221	ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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