Transcript: Human XR_001738691.2

PREDICTED: Homo sapiens RAS guanyl releasing protein 3 (RASGRP3), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RASGRP3 (25780)
Length:
2274
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001738691.2
NBCI Gene record:
RASGRP3 (25780)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001738691.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048115 CGGTCATCAACAAGCACATAA pLKO.1 1984 3UTR 100% 13.200 18.480 N RASGRP3 n/a
2 TRCN0000048113 GCTGCAATGAATTTCGATTAA pLKO.1 937 3UTR 100% 13.200 18.480 N RASGRP3 n/a
3 TRCN0000048116 CCAACGGCAATTACTGCAATT pLKO.1 1579 3UTR 100% 10.800 15.120 N RASGRP3 n/a
4 TRCN0000048114 GCCTCAGTCATAGTTCCATTT pLKO.1 1480 3UTR 100% 10.800 15.120 N RASGRP3 n/a
5 TRCN0000022729 GCAGAGTTTAATTTGGATCTT pLKO.1 999 3UTR 100% 4.950 6.930 N Rasgrp3 n/a
6 TRCN0000367958 CTGATGCACCGATGGTATTTA pLKO_005 858 3UTR 100% 15.000 10.500 N Rasgrp3 n/a
7 TRCN0000416206 ACGTCAGCCTCATCGACATAT pLKO_005 1081 3UTR 100% 13.200 9.240 N RASGRP3 n/a
8 TRCN0000048117 GCTAGTCAACTAGGATATGAA pLKO.1 1056 3UTR 100% 5.625 3.938 N RASGRP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001738691.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07939 pDONR223 100% 52% None (many diffs) n/a
2 ccsbBroad304_07939 pLX_304 0% 52% V5 (many diffs) n/a
3 TRCN0000476886 AGAAAACGGTCTCCACATATTAAG pLX_317 20.2% 52% V5 (many diffs) n/a
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