Construct: ORF ccsbBroad304_08117
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009020.1_s304c1, BRDN0000399080
- Derived from:
- ccsbBroadEn_08117
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BLNK (29760)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08117
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29760 | BLNK | B cell linker | NM_013314.3 | 90.6% | 1% | (many diffs) |
| 2 | human | 29760 | BLNK | B cell linker | XM_011539728.2 | 90.4% | 1% | (many diffs) |
| 3 | human | 29760 | BLNK | B cell linker | NM_001114094.1 | 85.7% | 1.1% | (many diffs) |
| 4 | human | 29760 | BLNK | B cell linker | XM_017016159.1 | 85.4% | 1.1% | (many diffs) |
| 5 | human | 29760 | BLNK | B cell linker | NM_001258440.1 | 79.5% | 1.2% | (many diffs) |
| 6 | human | 29760 | BLNK | B cell linker | NM_001258441.1 | 74.5% | 1.3% | (many diffs) |
| 7 | human | 29760 | BLNK | B cell linker | NR_047680.1 | 61.3% | (many diffs) | |
| 8 | human | 29760 | BLNK | B cell linker | NR_047682.1 | 58.2% | (many diffs) | |
| 9 | human | 29760 | BLNK | B cell linker | NR_047681.1 | 58.1% | (many diffs) | |
| 10 | human | 29760 | BLNK | B cell linker | NM_001258442.1 | 56.9% | 1.6% | (many diffs) |
| 11 | human | 29760 | BLNK | B cell linker | NR_047683.1 | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 139
- ORF end:
- 193
- ORF length:
- 54
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aaTAATTTTT TCCAAAAAAA 61 TTTTTTTTTT TTTTAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 121 AAAAAACTCG AGGCATCTAT GTCGGGTGCG GAGAAAGAGG TAATGAAATG GCACATGGTC 181 ATAGCTGTTT CCTGACgaag agcagcagtg gtccgatgac tttgacagcg actatgaaaa 241 tccagatgag cactcggact cagagatgta cgtgatgccc gccgaggaga acgctgatga 301 cagctacgag ccgcctccag tagagcagga aaccaggccg gttcacccag ccctgccctt 361 cgccagaggc gagtatatag acaatcgatc aagccagagg cattccccac ccttcagcaa 421 gacacttccc agtaagccca gctggccttc agagaaagca aggctcacct ccaccctgcc 481 ggccctgact gctttgcaga aacctcaagt cccacccaaa cccaaaggcc tccttgagga 541 tgaggctgat tatgtggtcc ccgtggaaga taatgatgaa aactatattc atcccacaga 601 aagcagttca cctccacctg aaaaagctcc catggtgaat agatcaacca agccaaattc 661 ctcaacgccc gcctctcctc caggaacagc ttcaggtcga aacagtgggg cctgggaaac 721 caagtcacct ccaccagctg caccatcccc gttgccacgg gccgggaaaa aaccaacgac 781 accactgaag acaactccag ttgcctctca acagaatgct tcaagtgttt gtgaagaaaa 841 acctatacct gctgaacgcc accgagggtc aagtcacaga caagaagctg tgcagtcacc 901 agtgtttcct cctgcccaga aacaaatcca ccaaaaaccc atacctctgc caagatttac 961 agaaggggga aacccaactg tggatgggcc cctacccagc ttttcatcta attccactat 1021 ttcagaacag gaagctggcg ttctctgcaa gccatggtat gctggagcct gtgatcgaaa 1081 gtctgctgaa gaggcattgc acagatcaaa caaggatgga tcatttctta ttcggaaaag 1141 ctctggccat gattccaaac aaccatatac actagttgta ttctttaata agcgagtata 1201 taatattcct gtgcgattta ttgaagcaac aaaacaatat gccttgggca gaaagaaaaa 1261 tggtgaagag tactttggaa gtgttgctga aatcatcagg aatcatcaac atagtccttt 1321 ggttcttatt gacagtcaga ataacacaaa agattccacc agactgaagt atgcagttaa 1381 agtttcataa acaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 1441 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 1501 caacctctgg atta