Construct: ORF ccsbBroad304_08151

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009881.1_s304c1, BRDN0000405263
Derived from:
ccsbBroadEn_08151
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STRN3 (29966)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08151
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29966 STRN3 striatin 3 NM_014574.4 99.9% 99.8% 1160A>G
2 human 29966 STRN3 striatin 3 XM_005267570.4 95% 94.8% 989_1099del;1271A>G
3 human 29966 STRN3 striatin 3 XM_005267569.4 93.7% 93.6% 988_1128del;1301A>G
4 human 29966 STRN3 striatin 3 NM_001083893.2 89.4% 89.3% 988_1239del;1412A>G
5 mouse 94186 Strn3 striatin, calmodulin bindin... NM_001172098.1 92.2% 96% (many diffs)
6 mouse 94186 Strn3 striatin, calmodulin bindin... XM_006516412.3 87.6% 91.2% (many diffs)
7 mouse 94186 Strn3 striatin, calmodulin bindin... XM_006516410.3 86.5% 90.1% (many diffs)
8 mouse 94186 Strn3 striatin, calmodulin bindin... XM_006516411.3 84.5% 87% (many diffs)
9 mouse 94186 Strn3 striatin, calmodulin bindin... NM_052973.2 82.5% 85.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2208
ORF length:
2139
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggacgagctt gccggaggcg gtggtggcgg cccggggatg gcggcccctc
121cccggcagca gcagggacct ggggggaacc tgggcctttc gcccgggggg aacggagcgg
181cgggcggcgg gggtcctccg gcctccgagg gagcgggtcc cgcggcaggc cccgagctgt
241cccggccgca gcagtacact atcccgggga tactgcacta catccagcac gagtgggctc
301ggttcgagat ggagcgggcg cactgggagg tggaacgggc cgaactgcag gcccggattg
361catttctaca aggcgaaaga aaaggtcaag agaacctgaa gaaggactta gtaagaagaa
421taaagatgtt agagtatgca ttaaaacaag aaagggcaaa atatcacaaa ttaaaatatg
481gcacggaact gaaccaaggt gacttgaaaa tgccaacctt tgagtcagaa gaaaccaaag
541acacagaggc tcccacagca cctcagaata gccagttaac gtggaagcaa ggcagacagc
601ttttaagaca gtatcttcag gaagtaggtt atacagatac aatattagat gtacggtctc
661agcgggtaag gtcattactt ggactatcta attcagaacc aaatggatca gtagaaacaa
721agaatttaga acagatcctg aatggaggtg aatctcctaa gcaaaaggga caagaaataa
781aaaggtcctc tggtgatgtt cttgagacgt tcaatttctt agaaaatgcc gatgacagtg
841atgaagatga ggaaaatgac atgatcgaag gcatcccaga aggaaaagac aaacatcgga
901tgaataaaca taaaataggt aatgaaggtt tagctgctga cctaactgac gatcctgata
961ctgaggaagc actgaaagaa tttgattttt tagtgactgc tgaagatggt gaaggagctg
1021gagaagcacg gagttcgggg gatggcacag aatgggctga accaataacg tttccatctg
1081gaggaggcaa gtcatttatt atgggttctg atgatgtttt gttaagtgta ctgggccttg
1141gagaccttgc agacttgacg gtaacaaatg atgcagacta tagttatgat ttgcctgcta
1201ataaagatgc ctttcgaaag acatggagtc ccaagtatac actacgtagc cattttgatg
1261gagtacgggc attagctttt catcctgtag aacctgtgct ggttactgct tctgaggacc
1321ataccctgaa actttggaac ctgcaaaaaa cagttcctgc caaaaagagt gcctctttag
1381atgtagagcc tatctacaca tttagggccc acatcggccc tgttctgtca ttagctatta
1441gttctaatgg agaacagtgt tttagtggtg gtattgatgc aaccatccag tggtggaata
1501tgccgagtcc cagtgtagat ccatatgata catatgagcc aaatgttcta gctggcactt
1561tagttggtca tacagatgca gtttggggtc ttgcttatag tggcataaaa aatcaattac
1621tgtcttgttc agcagatggc actgttaggt tatggaatcc acaagaaaaa ttgccatgta
1681tttgcactta caatggagat aaaaagcatg gaatacctac atcagttgac tttataggct
1741gtgatccagc tcatatggta acctctttca acactggtag tgcagtaatt tatgatttag
1801aaacatcaca gtcattggtg atactttcat cacaggtaga ttctggttta caatctaata
1861atcatatcaa cagagtagta agtcatccca cacttcctgt tacaataact gctcatgaag
1921atagacacat caaatttttt gacaataaaa cgggtaaaat gatccattct atggtagctc
1981acttggatgc tgttacaagt ctagcagtag atcctaatgg aatctatttg atgtctggaa
2041gccatgactg ttccatcaga ttatggaatt tagacagcaa gacatgtgtg caagaaataa
2101cagctcacag aaagaaattg gatgaatcaa tttatgatgt tgctttccac tcgtcaaaag
2161catatatagc tagtgcagga gctgatgctc ttgccaaagt atttgtattg ccaactttct
2221tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
2281aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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