Construct: ORF ccsbBroad304_08269

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008260.1_s304c1, BRDN0000406170
Derived from:
ccsbBroadEn_08269
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DNAJC27 (51277)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08269
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51277 DNAJC27 DnaJ heat shock protein fam... NM_016544.3 99.8% 100% 33C>T
2 human 51277 DNAJC27 DnaJ heat shock protein fam... XM_011532902.1 69.3% 64% (many diffs)
3 human 51277 DNAJC27 DnaJ heat shock protein fam... NM_001198559.1 64.3% 64.4% 33C>T;528_529delGG;531_532ins290
4 human 51277 DNAJC27 DnaJ heat shock protein fam... XR_244938.2 16.2% (many diffs)
5 mouse 217378 Dnajc27 DnaJ heat shock protein fam... NM_153082.4 89.7% 93.4% (many diffs)
6 mouse 217378 Dnajc27 DnaJ heat shock protein fam... XM_006515052.3 82.2% 85.5% (many diffs)
7 mouse 217378 Dnajc27 DnaJ heat shock protein fam... XR_381183.3 57.5% (many diffs)
8 mouse 217378 Dnajc27 DnaJ heat shock protein fam... XR_381184.3 17.3% (many diffs)
9 mouse 217378 Dnajc27 DnaJ heat shock protein fam... XR_381181.3 16% (many diffs)
10 mouse 217378 Dnajc27 DnaJ heat shock protein fam... XR_381182.3 15.7% (many diffs)
11 mouse 217378 Dnajc27 DnaJ heat shock protein fam... XR_872399.2 7.9% (many diffs)
12 mouse 217378 Dnajc27 DnaJ heat shock protein fam... XR_872397.2 7.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
885
ORF length:
819
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggaatgga ggccaacatg ccgaagcgga aggagcctgg caggtctctc cgcatcaaag
121tcatctccat gggcaacgcc gaagtgggga aaagctgtat tataaagcga tactgtgaga
181aaagattcgt gtctaaatac ctggcaacaa ttggaattga ctatggagtc acaaaggtac
241acgtcagaga cagagaaatc aaagttaaca tctttgatat ggctggacat cccttcttct
301atgaggttcg aaatgagttt tacaaggaca cacagggtgt gatactggtc tatgatgttg
361ggcagaaaga ctcctttgac gcccttgatg cgtggctggc agaaatgaag caagagcttg
421gacctcatgg aaacatggaa aatattatat ttgtagtttg tgccaacaag attgattgta
481ccaaacatcg ctgtgtagat gaaagtgaag gacgtctttg ggctgaaagc aaagggttcc
541tgtactttga aacttcagca caaactggag aaggcattaa tgagatgttc cagacctttt
601atatatccat agttgattta tgtgaaaatg gcgggaaacg ccctaccacc aatagcagtg
661ctagtttcac caaagaacaa gcagatgcca ttcgcagaat tcgaaatagt aaagacagtt
721gggacatgct gggagtcaaa cctggggcct caagggatga agtcaataaa gcgtatcgga
781aacttgctgt gcttcttcac cctgacaaat gtgtagcacc tggcagtgaa gatgccttca
841aagcagttgt gaatgctcgg acagccctcc tgaaaaacat caagtaccca actttcttgt
901acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
961gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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