Construct: ORF ccsbBroad304_08310
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004954.1_s304c1, BRDN0000406275
- Derived from:
- ccsbBroadEn_08310
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VTA1 (51534)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08310
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51534 | VTA1 | vesicle trafficking 1 | NM_016485.5 | 100% | 100% | |
| 2 | human | 51534 | VTA1 | vesicle trafficking 1 | NM_001286371.1 | 91.2% | 91.2% | 696_697ins81 |
| 3 | human | 51534 | VTA1 | vesicle trafficking 1 | NM_001286372.1 | 72.3% | 69.3% | 0_1ins79;31_32ins95;522_523ins81 |
| 4 | mouse | 66201 | Vta1 | vesicle (multivesicular bod... | NM_025418.3 | 86.6% | 90.9% | (many diffs) |
| 5 | mouse | 66201 | Vta1 | vesicle (multivesicular bod... | XM_006512809.3 | 80.8% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 987
- ORF length:
- 921
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcgcttgca ccgctgcccc cgctccccgc acagttcaag agcatacagc 121 atcatctgag gacggctcag gagcatgaca agcgagaccc tgtggtggct tattactgtc 181 gtttatacgc aatgcagact ggaatgaaga tcgatagtaa aactcctgaa tgtcgcaaat 241 ttttatcaaa gttaatggat cagttagaag ctctaaagaa gcagttgggt gataatgaag 301 ctattactca agaaatagtg ggctgtgccc atttggagaa ttatgctttg aaaatgtttt 361 tgtatgcaga caatgaagat cgtgctggac gatttcacaa aaacatgatc aagtccttct 421 atactgcaag tcttttgata gatgtcataa cagtatttgg agaactcact gatgaaaatg 481 tgaaacacag gaagtatgcc agatggaagg caacatacat ccataattgt ttaaagaatg 541 gggagactcc tcaagcaggc cctgttggaa ttgaagaaga taatgatatt gaagaaaatg 601 aagatgctgg agcagcctct ctgcccactc agccaactca gccatcatca tcttcaactt 661 atgacccaag caacatgcca tcaggcaact atactggaat acagattcct ccgggtgcac 721 acgctccagc taatacacca gcagaagtgc ctcacagcac aggtgtagca agtaatacta 781 tccaacctac tccacagact atacctgcca ttgatcccgc acttttcaat acaatttccc 841 agggggatgt tcgtctaacc ccagaagact ttgctagagc tcagaagtac tgcaaatatg 901 ctggcagtgc tttgcagtat gaagatgtaa gcactgctgt ccagaatcta caaaaggctc 961 tcaagttact gacgacaggc agagaatgcc caactttctt gtacaaagtg gttggtaagc 1021 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1081 ggcgcgttaa gtcgacaatc aacctctgga tta