Construct: ORF ccsbBroad304_08383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008641.1_s304c1, BRDN0000410114
- Derived from:
- ccsbBroadEn_08383
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NLE1 (54475)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08383
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54475 | NLE1 | notchless homolog 1 | NM_018096.5 | 99.7% | 99.3% | 16C>G;506G>A;955C>A |
| 2 | human | 54475 | NLE1 | notchless homolog 1 | NM_001014445.2 | 39.7% | 39.5% | 0_1ins876;79C>A |
| 3 | human | 54475 | NLE1 | notchless homolog 1 | XM_017024777.1 | 39.7% | 39.5% | 0_1ins876;79C>A |
| 4 | mouse | 217011 | Nle1 | notchless homolog 1 (Drosop... | NM_145431.2 | 89.2% | 95.2% | (many diffs) |
| 5 | mouse | 217011 | Nle1 | notchless homolog 1 (Drosop... | XR_879591.1 | 70% | (many diffs) | |
| 6 | mouse | 217011 | Nle1 | notchless homolog 1 (Drosop... | XR_388409.2 | 69.9% | (many diffs) | |
| 7 | mouse | 217011 | Nle1 | notchless homolog 1 (Drosop... | XM_006532975.2 | 58.4% | 62.2% | (many diffs) |
| 8 | mouse | 217011 | Nle1 | notchless homolog 1 (Drosop... | XM_006532976.2 | 51.3% | 54% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1521
- ORF length:
- 1455
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagcagtg gcggacgagg cggtggcgcg cgatgtgcag cggttgctag 121 tgcagttcca ggatgagggc gggcagctgc tgggttcccc gttcgacgtg cccgtggaca 181 tcaccccgga caggctgcag ctcgtgtgca acgcgctact ggcccaggag gatcccctgc 241 cactggcttt ctttgtccac gatgctgaga tcgtctcctc actggggaag acgttggagt 301 cccaggcagt ggagacagag aaggtcctag acatcatcta ccagccacag gctatcttca 361 gagtccgggc tgtgactcgc tgcaccagct ccttggaggg tcacagtgag gcagtcattt 421 ctgtggcctt cagccctacg ggaaagtacc tggccagtgg ctctggagac accaccgtgc 481 gcttctggga tctcagcaca gagacaccac atttcacatg caagggacac agacactggg 541 tccttagtat atcctggtct ccagatggca agaagctggc ctcaggctgc aagaatggcc 601 agattctcct ctgggaccca agcacaggga agcaggtggg caggaccctc gctggccaca 661 gcaagtggat cacaggcctg agctgggagc ccctccatgc gaaccctgag tgccgctatg 721 tggccagcag ctccaaggat ggcagtgtgc ggatctggga cacaactgca ggccgctgtg 781 agcgcatcct caccgggcac acccagtcgg tcacctgtct ccggtgggga ggggacgggc 841 ttctctactc tgcctcccag gaccgcacca tcaaagtctg gagagctcat gacggtgtgc 901 tgtgccggac tctgcaaggc cacggccact gggtgaacac catggccctc agcactgact 961 atgccctgcg cactggggcc tttgaacctg ctgaggcctc agttaatccc caagacctca 1021 aaggatcctt gcaggagttg aaggagaggg ctctgagccg atacaacctc gtgcggggcc 1081 agggtccaga gaggctggtg tctggctccg acgacttcac cttattcctg tggtccccag 1141 cagaggacaa aaagcctctc actcggatga caggacacca agctctcatc aaccaggtgc 1201 tcttctctcc tgactcccgc atcgtggcta gtgcctcctt tgacaagtcc atcaagctgt 1261 gggatggcag gacgggcaag tacctggctt ccctacgcgg ccacgtggct gccgtgtacc 1321 agattgcgtg gtcagctgac agtcggctcc tggtcagcgg cagcagtgac agcacactga 1381 aggtgtggga tgtgaaggcc cagaagctgg ccatggacct gcccggccac gcggatgagg 1441 tatatgctgt tgactggagt ccagatggcc agagagtggc aagtggtggg aaggacaaat 1501 gcctccggat atggaggaga tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1561 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1621 ttaagtcgac aatcaacctc tggatta