Construct: ORF ccsbBroad304_08456
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009031.1_s304c1, BRDN0000461956
- Derived from:
- ccsbBroadEn_08456
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEATR3 (55027)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08456
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55027 | HEATR3 | HEAT repeat containing 3 | NM_182922.4 | 99.9% | 100% | 591T>C;1888C>T |
| 2 | human | 55027 | HEATR3 | HEAT repeat containing 3 | XM_005256013.2 | 87.2% | 84.9% | (many diffs) |
| 3 | human | 55027 | HEATR3 | HEAT repeat containing 3 | XR_002957828.1 | 87.2% | (many diffs) | |
| 4 | human | 55027 | HEATR3 | HEAT repeat containing 3 | NM_001329729.2 | 82.6% | 82.7% | 0_1ins351;240T>C;1537C>T |
| 5 | human | 55027 | HEATR3 | HEAT repeat containing 3 | NM_001329730.2 | 82.6% | 82.7% | 0_1ins351;240T>C;1537C>T |
| 6 | human | 55027 | HEATR3 | HEAT repeat containing 3 | NM_001329731.2 | 65.9% | 66% | 0_1ins693;1195C>T |
| 7 | human | 55027 | HEATR3 | HEAT repeat containing 3 | XM_017023386.1 | 65.9% | 66% | 0_1ins693;1195C>T |
| 8 | human | 55027 | HEATR3 | HEAT repeat containing 3 | NR_138093.2 | 43.4% | (many diffs) | |
| 9 | human | 55027 | HEATR3 | HEAT repeat containing 3 | NR_138092.2 | 43.3% | (many diffs) | |
| 10 | mouse | 234549 | Heatr3 | HEAT repeat containing 3 | NM_172757.3 | 84.4% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2109
- ORF length:
- 2040
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaagagc cggacgaagc gcttcaagcg acctcagttc tcccctacgg 121 gcgactgtca ggccgaggcg gctgcggcgg cgaatgggac cggaggcgag gaggacgacg 181 ggccggcggc ggagctgctg gaaaagctcc agcacccgag cgccgaggtc cgcgagtgcg 241 cctgcgcagg gctggcccgg ctggtgcagc agcggccggc actcccgggc ctggcgcgac 301 gagacgccgt gcgccgcctc gggccgctgc tgctagaccc cagcctggcc gtcagggaga 361 ctgcagccgg cgcgctgaga aatctcagtg cttgtggagg ttttgaagtt tgtgatgaca 421 tggtgactaa ggatatcatg acccctctgg ttgcgctgct aaaagagtgt agtgctggac 481 tggattcaaa tgagatgtct ctgcaggaga aaaaagatca gaacagaaat tctattgaga 541 acatagccaa tgagactgtg aacgtgctgt ggaatatatg tgaatgcagt agtagagcag 601 tgtctatatt caacaaagaa gggtgtttgg agattgtgtt aaagtattta agtaggttcc 661 ctaccaatgt tgacctggct atttcagtag catattgttt gcagacagtg actgaggata 721 acccagagct gctgaagtct ttcagtgcta cagcattgaa catgctggaa tcagcactgc 781 tttctcctgt cagttccatg gaatctcttc tattgaagac attggtagca ggcacaattt 841 ggaatctaaa ggacattatt ccatgcaaga gtcaagcaga aatcataaat gccttactaa 901 agatcttatc tgaagttttg ggaatggatg ctggtgaaat ggttattcaa atgaaagagg 961 ctgaaacgca aaggttaaaa actgctgcag aggctgagga aatattagag aacactaatg 1021 gggatgattt gattgaagat gatgaaatgg aaggaatttc tcataaaaga agagtcagaa 1081 ggaaaacttt cgtttcagat ttacttccgc ccactgacaa ggaactgaga gagactatag 1141 cattgctgac agcccaacag actgctctgg aaattattgt caacatgtgc tgcaatgaag 1201 atccctctga tgacgaatgg gaagagcttt ctagcagtga tgaaagtgac gcatttatgg 1261 agaattcctt cagtgagtgc gggggacagc tgttttctcc cctctgcctc tcccatgaag 1321 ttcacacggc tctcaccaac tacctcatcc caaagaagat ttttgagaaa actgcctttc 1381 caaacagcat tgcagttgac ctatgttcta ggaaccctac ttggaaacct ttgattagaa 1441 aaatgaacac tattcagtgc agagccctct tctgtctcca gagtcttgtg tccctcctgg 1501 atgtggagca cctgggagga gccgcagccc ttcagacgct tgcacagcat ctgtcacagc 1561 tgcttttttc tcaaccagat tttgctaaac atgttgactt tctagaagcc ataagtagtg 1621 ctttgagggc ccttttgcaa acaatggcct ccaagaacat ttcccagtgc atgactcctg 1681 atcagctgat gacattatgc aaagcaggca ttcatagtag taatgtcggg gttagagtga 1741 atgtcgttag cattttagga atcactggca gcgtccttgc caaagaagat ggtacacttg 1801 aaactcttaa gaacattggg tgctttctgc ttgaagttac caccaaagat ccttcccttg 1861 tggtagcagg agaagctttg gatgccctct ttgatgtttt tgcagatggt aaagaagccg 1921 aaagagcctc gattcaaatt aaattattat ctgctttgaa agaattccag ccggtcttta 1981 aaatgaagat acgtaaagaa gggagaggta actatagcac agatcagctg tgtgttcttg 2041 acaatgtgaa aatgaatttg cgaagattta ttgcttatca agaaactgtt gagaaaagac 2101 tgacttcttt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 2161 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2221 tcaacctctg gatta