Construct: ORF ccsbBroad304_08472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005062.1_s304c1, BRDN0000407211
- Derived from:
- ccsbBroadEn_08472
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR60 (55112)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08472
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55112 | WDR60 | WD repeat domain 60 | NM_018051.5 | 99.9% | 99.8% | 675C>G;875C>T;2073A>G |
| 2 | human | 55112 | WDR60 | WD repeat domain 60 | XM_006716041.2 | 99.6% | 99.5% | (many diffs) |
| 3 | human | 55112 | WDR60 | WD repeat domain 60 | XM_011516367.2 | 99.4% | 99.1% | (many diffs) |
| 4 | human | 55112 | WDR60 | WD repeat domain 60 | XM_017012382.1 | 95.7% | 95.6% | (many diffs) |
| 5 | human | 55112 | WDR60 | WD repeat domain 60 | NM_001350914.1 | 95.5% | 95.4% | (many diffs) |
| 6 | human | 55112 | WDR60 | WD repeat domain 60 | XM_011516368.2 | 95.5% | 95.4% | (many diffs) |
| 7 | human | 55112 | WDR60 | WD repeat domain 60 | XM_011516369.2 | 95.3% | 95.2% | (many diffs) |
| 8 | human | 55112 | WDR60 | WD repeat domain 60 | XM_017012381.2 | 95.3% | 95.2% | (many diffs) |
| 9 | human | 55112 | WDR60 | WD repeat domain 60 | XR_242045.2 | 83.8% | (many diffs) | |
| 10 | human | 55112 | WDR60 | WD repeat domain 60 | NM_001350915.2 | 81.9% | 71.1% | (many diffs) |
| 11 | human | 55112 | WDR60 | WD repeat domain 60 | XM_005249549.1 | 81.7% | 70.9% | (many diffs) |
| 12 | human | 55112 | WDR60 | WD repeat domain 60 | XR_001744826.1 | 79.9% | (many diffs) | |
| 13 | human | 55112 | WDR60 | WD repeat domain 60 | XR_927486.2 | 79.8% | (many diffs) | |
| 14 | human | 55112 | WDR60 | WD repeat domain 60 | XR_001744828.1 | 79.6% | (many diffs) | |
| 15 | human | 55112 | WDR60 | WD repeat domain 60 | XR_428179.2 | 78% | (many diffs) | |
| 16 | human | 55112 | WDR60 | WD repeat domain 60 | XR_001744827.1 | 77.7% | (many diffs) | |
| 17 | human | 55112 | WDR60 | WD repeat domain 60 | XR_002956461.1 | 75% | (many diffs) | |
| 18 | human | 55112 | WDR60 | WD repeat domain 60 | NM_001350917.2 | 59.4% | 58% | (many diffs) |
| 19 | human | 55112 | WDR60 | WD repeat domain 60 | NM_001350918.2 | 59.4% | 58% | (many diffs) |
| 20 | human | 55112 | WDR60 | WD repeat domain 60 | XR_001744824.1 | 59.2% | (many diffs) | |
| 21 | human | 55112 | WDR60 | WD repeat domain 60 | XR_001744825.1 | 55.6% | (many diffs) | |
| 22 | human | 55112 | WDR60 | WD repeat domain 60 | XR_001744823.1 | 54.6% | (many diffs) | |
| 23 | human | 55112 | WDR60 | WD repeat domain 60 | NM_001350916.2 | 54.2% | 54.3% | 0_1ins1461;612A>G |
| 24 | human | 55112 | WDR60 | WD repeat domain 60 | XM_017012388.1 | 54.2% | 54.3% | 0_1ins1461;612A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3264
- ORF length:
- 3198
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gcccgggaag agaagaacca aagatgatac ctggaaagca gatgacctca 121 gaaaacatct ctgggccata cagtcaggtg gttccaagga agaaagaaag cacagagaga 181 agaagctgcg taaggagtct gagatggacc ttcctgaaca taaggagccg aggtgcaggg 241 atcccgacca ggatgccagg agcagagaca gggtggccga agtccacacc gctaaggaga 301 gtcctcgtgg ggagagggac agagacagac agagggagag gagaagagac gcaaaagacc 361 gggagaaaga aaagctgaag gagaaacatc gagaggcaga aaagtctcac agcagaggaa 421 aggacaggga aaaagaaaaa gacagaaggg cccggaagga agagctccgg cagaccgtgg 481 cccaccacaa cctgctgggc caggagacac gcgaccggca gctcctggag cgggcggaga 541 ggaaaggccg ctcagtaagt aaagtaagaa gtgaagagaa agatgaagac tctgaaagag 601 gagatgaaga tagagaaaga agataccgag aaagaaagct gcagtacgga gacagcaagg 661 acaaccctct caagtactgg ctttataaag aagaaggcga gaggagacac aggaagccca 721 gagagccaga tcgagacaag aaacaccgag aaaaaagcag cacaagggaa aaaagagaga 781 aatattccaa agagaaaagt aattcattct ctgacaaagg ggaagaaaga cataaagaaa 841 agcgacacaa agaaggtttt cattttgatg atgagaggca ccaaagcaac gtggatagaa 901 aagagaaatc ggcaaaagat gagcccagga aaagggaatt ccagaatggt gaacacagaa 961 atcgaggtgc aagctcaaaa agagatggga ccagcagcca gcatgctgag aatttagtaa 1021 ggaatcatgg aaaagataaa gattcaagac ggaagcatgg ccacgaggaa ggctcttctg 1081 tgtggtggaa gctggaccag aggccgggag gcgaggaaac cgtggaaatt gaaaaggaag 1141 aaactgattt agaaaatgct agagctgatg catatacagc cagttgtgaa gatgattttg 1201 aagactatga agatgacttt gaggtttgtg atggtgatga tgatgaaagc agtaatgaac 1261 ctgagtcaag agaaaaactg gaagaacttc ctctagctca aaaaaaggaa atacaagaaa 1321 ttcaaagagc tattaatgca gaaaatgaaa ggattggcga gttatctttg aaactgtttc 1381 agaagcgagg tagaacagaa tttgaaaagg agcccaggac agatacaaac agttcccctt 1441 ccagagcctc tgtttgtgga atttttgtgg attttgcctc agcttcacac cgtcaaaaga 1501 gtcggactca ggcccttaag caaaagatgc gaagtacaaa actgcttcgg ctcattgact 1561 tagatttttc atttactttc tctctcttgg atctaccacc agtaaatgaa tatgacatgt 1621 atatcagaaa ctttgggaaa aaaaatacca agcaggcata tgttcagtgt aacgaagata 1681 atgttgaaag agacattcaa acggaggaaa tagagaccag ggaagtgtgg acccagcacc 1741 cgggagaaag tactgttgta tctggaggca gtgaacaaag agatacctct gatgctgtag 1801 ttatgccaaa gattgatact ccaaggttat gtagctttct gcgggctgct tgtcaggtga 1861 tggccgtttt gctggaagag gatcgcttgg cagctgaacc cagctggaat cttagggctc 1921 aagacagggc cctgtatttt agtgacagct catctcagct gaacaccagt ctaccattcc 1981 ttcaaaatcg aaaagtatcc tccttgcaca cctcccgagt tcagaggcag atggtggtct 2041 ccgttcacga cttacccgag aagagctttg tgcccctgct ggacagcaaa tacgtcctct 2101 gtgtgtggga tatttggcag ccttcagggc cacagaaggt tctgatatgt gagtcccagg 2161 tcacgtgttg ctgcttgagc cctttgaaag catttttact gtttgccgga acagcgcacg 2221 gctcagttgt cgtctgggat ttgagagaag actcaaggct gcattactct gtgacgctga 2281 gcgatggctt ctggacgttc cggaccgcca cgttttccac cgatggaatc cttacctcag 2341 taaaccaccg aagccctctt caagcagtag aacctatctc aacgtccgtc cacaaaaagc 2401 agagctttgt gctttcaccc ttttctactc aagaagaaat gtcaggtttg tccttccaca 2461 tcgcttcctt ggatgagagt ggggttctca atgtatgggt ggttgttgaa ttaccaaagg 2521 cagacatcgc aggttcaata agtgatttag gtctgatgcc tggagggagg gtcaagctgg 2581 tacatagtgc tctgatccag ttgggtgaca gtctttccca taaaggtaat gaattttggg 2641 gcactacaca aacactgaat gttaaatttc tgccttcaga tcctaatcac tttattattg 2701 gcacagacat gggtctcata agccatggca caagacaaga tttgagagtg gctcccaaac 2761 tattcaaacc tcagcaacat ggtataagac cagtgaaagt taatgtcatt gatttttcac 2821 catttggaga accaatattt ttggccggct gttcggacgg aagcatcagg ctgcaccagc 2881 tgagctccgc gtttccgctc ctgcagtggg acagcagcac ggacagccat gcggtcaccg 2941 gcctgcagtg gtccccaacc aggcctgccg tgttcctggt gcaggacgac acatccaaca 3001 tctacatctg ggacctcctc cagagcgatc tgggtcctgt cgccaaacag caggtctccc 3061 ccaacaggct ggtggccatg gctgcggtgg gtgagcctga gaaggctggt ggcagcttcc 3121 tggccctggt gctggccagg gcgtctggct ccatcgacat ccagcacctg aagaggcggt 3181 gggcggcccc ggaggtggac gagtgcaaca ggctgcgtct gcttttgcag gaagccctgt 3241 ggccagaggg aaaactgcac aagcacccaa ctttcttgta caaagtggtt ggtaagccta 3301 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 3361 gcgttaagtc gacaatcaac ctctggatta