Construct: ORF ccsbBroad304_08501

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016061.1_s304c1, BRDN0000460332
Derived from:
ccsbBroadEn_08501
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NUDT15 (55270)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08501
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55270 NUDT15 nudix hydrolase 15 NM_018283.4 99.7% 100% 384A>G
2 human 55270 NUDT15 nudix hydrolase 15 NM_001304745.1 79.9% 65.7% (many diffs)
3 human 55270 NUDT15 nudix hydrolase 15 NR_136688.1 16.8% 1_180del;564A>G;673_2921del
4 human 55270 NUDT15 nudix hydrolase 15 NR_136687.1 16.7% 1_180del;564A>G;673_2928del
5 mouse 214254 Nudt15 nudix (nucleoside diphospha... NM_172527.2 80.3% 85.3% (many diffs)
6 mouse 214254 Nudt15 nudix (nucleoside diphospha... XM_017315941.1 80.3% 85.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
561
ORF length:
492
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gacggccagc gcacagccgc gcgggcggcg gccaggagtc ggagtcggag
121tcgtggtgac cagctgcaag catccgcgtt gcgtcctcct ggggaagagg aaaggctcgg
181ttggagctgg cagtttccaa ctccctggag gtcatctgga gttcggtgaa acctgggaag
241aatgtgctca aagggaaacc tgggaagaag cagctcttca cctgaaaaat gttcactttg
301cctcagttgt gaattctttc attgagaagg agaattacca ttatgttact atattaatga
361aaggagaagt ggatgtgact catgattcag aaccaaagaa tgtagagcct gaaaaaaatg
421aaagttggga gtgggttcct tgggaagaac tgcctcccct ggaccagctt ttctggggac
481tgcgttgttt aaaagaacaa ggctatgatc catttaaaga agatctgaac catctggtgg
541gatacaaagg aaatcatctc ttgccaactt tcttgtacaa agtggttggt aagcctatcc
601ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
661ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)