Construct: ORF ccsbBroad304_08529
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006181.1_s304c1, BRDN0000403492
- Derived from:
- ccsbBroadEn_08529
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK32B (55351)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08529
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55351 | STK32B | serine/threonine kinase 32B | NM_018401.3 | 99.8% | 99.5% | 14A>T;592A>G |
| 2 | human | 55351 | STK32B | serine/threonine kinase 32B | NM_001345969.2 | 92.5% | 92.2% | 14A>T;471_472ins90;502A>G |
| 3 | human | 55351 | STK32B | serine/threonine kinase 32B | NM_001306082.2 | 88.5% | 88.4% | 0_1ins141;451A>G |
| 4 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_024454131.1 | 88.5% | 88.4% | 0_1ins141;451A>G |
| 5 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_017008374.1 | 54.2% | 51.9% | (many diffs) |
| 6 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_005247982.3 | 52.8% | 52.6% | 0_1ins585;7A>G |
| 7 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_005247983.4 | 52.8% | 52.6% | 0_1ins585;7A>G |
| 8 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_011513504.2 | 52.8% | 52.6% | 0_1ins585;7A>G |
| 9 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_017008377.1 | 52.8% | 52.6% | 0_1ins585;7A>G |
| 10 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_017008375.1 | 49.8% | 42.5% | (many diffs) |
| 11 | human | 55351 | STK32B | serine/threonine kinase 32B | XM_017008376.1 | 49.8% | 42.5% | (many diffs) |
| 12 | mouse | 64293 | Stk32b | serine/threonine kinase 32B | NM_022416.2 | 86.6% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg cgggaacctc tcccacaagc cccccgtgtt tgacgagaat gaggaagtca 121 actttgacca ttttcagatt ctgcgggcca ttggtaaagg gagttttgga aaggtatgca 181 tcgtgcagaa gcgagacact aagaaaatgt atgcaatgaa gtacatgaac aagcagaagt 241 gcatcgagag ggatgaggtt cggaatgttt tccgggagct gcagatcatg caagggctgg 301 agcacccctt cctggtcaat ctgtggtact ccttccagga tgaggaggac atgttcatgg 361 tggtggacct gctcctggga ggcgacctgc gctaccatct gcagcagaat gtgcatttca 421 cagaggggac tgtgaaactc tacatctgtg agctggcact ggccctggag tatcttcaga 481 ggtaccacat catccacaga gacatcaagc cagacaatat cctgctggat gaacacggac 541 atgttcacat tacagacttc aacatagcga cggtagtgaa aggagcagaa agggcttcct 601 ccatggctgg caccaagccc tacatggctc cagaagtatt ccaggtgtac atggacggag 661 gccccggata ctcgtaccct gtcgactggt ggtccctggg catcacagcc tatgagctgc 721 tgcggggctg gaggccgtac gaaatccact cggtcacgcc catcgatgaa atcctcaaca 781 tgttcaaggt ggagcgtgtc cactactcct ccacgtggtg caaggggatg gtggccctgc 841 tgaggaagct cctgaccaag gatcctgaga gccgcgtgtc cagccttcat gacatacaga 901 gcgtgcccta cttggccgac atgaactggg acgcggtgtt caagaaggca ctgatgcccg 961 gctttgtgcc caataaaggg aggttgaact gcgatcccac atttgagctt gaagagatga 1021 ttctagaatc caagccactt cacaaaaaga agaagcgatt ggcaaagaac agatccaggg 1081 atggcacaaa ggacagctgc ccgctgaatg gacacctgca gcactgtttg gagactgtcc 1141 gggaggaatt catcatattc aacagagaga agctcaggag gcagcaggga cagggcagcc 1201 agctcttgga caccgacagc cgagggggag gccaggccca aagcaagctc caggacgggt 1261 gcaacaacaa cctcctcacc cacacctgca cccgtggctg cagcagctgc ccaactttct 1321 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1381 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta