Construct: ORF ccsbBroad304_08651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013894.1_s304c1, BRDN0000462225
- Derived from:
- ccsbBroadEn_08651
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FSTL5 (56884)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08651
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56884 | FSTL5 | follistatin like 5 | NM_020116.5 | 99.9% | 99.7% | 1699A>T;1891C>A |
| 2 | human | 56884 | FSTL5 | follistatin like 5 | NM_001128427.2 | 99.8% | 99.6% | 124_125insAGC;1696A>T;1888C>A |
| 3 | human | 56884 | FSTL5 | follistatin like 5 | XM_011532126.1 | 98.8% | 98.5% | 1312_1313ins27;1672A>T;1864C>A |
| 4 | human | 56884 | FSTL5 | follistatin like 5 | NM_001128428.2 | 98.7% | 98.4% | (many diffs) |
| 5 | mouse | 213262 | Fstl5 | follistatin-like 5 | NM_001253719.1 | 87.3% | 91% | (many diffs) |
| 6 | mouse | 213262 | Fstl5 | follistatin-like 5 | NM_178673.4 | 87.3% | 91% | (many diffs) |
| 7 | mouse | 213262 | Fstl5 | follistatin-like 5 | XM_017319517.1 | 87.1% | 90.9% | (many diffs) |
| 8 | mouse | 213262 | Fstl5 | follistatin-like 5 | XM_006501262.1 | 86.2% | 89.8% | (many diffs) |
| 9 | mouse | 213262 | Fstl5 | follistatin-like 5 | XM_006501263.1 | 86.1% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2610
- ORF length:
- 2541
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttaagtgc tggtcagttg tcttggttct cggattcatt tttctggagt 121 cggaaggaag gccaaccaaa gaaggaggat atggccttaa atcctatcag cctctaatga 181 gattgcgaca taagcaggaa aaaaatcaag aaagttcaag agtcaaagga tttatgattc 241 aggatggccc ttttggatct tgtgaaaata agtactgtgg tttgggaaga cactgtgtta 301 ccagcagaga gacagggcaa gcagaatgtg cctgtatgga cctttgcaaa cgtcactaca 361 aacctgtgtg tggatctgac ggagaattct atgaaaacca ctgtgaagtg cacagagctg 421 cttgcctgaa aaaacaaaag attaccattg ttcacaatga agactgcttc tttaaaggag 481 ataagtgcaa gactactgaa tacagcaaga tgaaaaatat gctattagat ttacaaaatc 541 aaaaatatat tatgcaagaa aatgaaaatc ctaatggcga cgacatatct cggaagaagc 601 tattggtgga tcaaatgttt aaatattttg atgcagacag taatggactt gtagatatta 661 atgaactaac tcaggtgata aaacaggaag aacttggcaa ggatctcttt gattgtactt 721 tgtatgttct attgaaatat gatgatttta atgctgacaa gcacctggct cttgaagaat 781 tttatagagc attccaagtg atccagttga gtctgccaga agatcagaaa ctaagcatca 841 ctgcagcaac tgtgggacaa agtgctgttc tgagctgtgc cattcaagga accctgagac 901 ctcccattat ctggaaaagg aacaatatta ttctaaataa tttagatttg gaagacatca 961 atgactttgg agatgatggg tccttgtata ttactaaggt taccacaact cacgttggca 1021 attacacctg ctatgcagat ggctatgaac aagtctatca gactcacatc ttccaagtga 1081 atgttcctcc agtcatccgg gtgtatccag agagtcaggc tagagagcct ggggtaactg 1141 ccagtcttag gtgccatgca gagggcatac caaagcctca gcttggctgg ttgaagaatg 1201 gaattgatat tacaccaaag ctttccaaac aactcacgct tcaagcaaat ggcagtgagg 1261 ttcacataag caatgtgcgc tatgaagata ctggagcata cacttgtatc gcaaagaatg 1321 aagcaggagt ggatgaagac atctcttctc tttttgtgga agactctgct agaaagaccc 1381 tagctaacat attatggaga gaagaaggtc tgggaattgg gaacatgttc tatgtttttt 1441 atgaagatgg aatcaaagtg atacaaccca tagaatgtga atttcagagg cacattaagc 1501 ctagtgaaaa gctccttgga tttcaggatg aagtctgtcc caaagctgag ggagatgaag 1561 ttcagaggtg tgtgtgggca tcagctgtta atgtcaaaga caagttcatt tatgttgcac 1621 agccaacttt ggacagagtc cttattgttg atgtgcagtc ccaaaaagtt gttcaggcag 1681 tgagcacaga ccctgtccca gttaaattac actatgacaa atcacatgat caggtctggg 1741 tgctaagctg gggtaccttg gagaagtcat caccaacact acaggtaatt accctggcca 1801 gtgggaatgt gcctcaccac acgatccaca cccaaccagt gggaaagcaa tttgacagag 1861 tggatgattt tttcattccc accacaacac tcattatcac ccatatgagg tttggattta 1921 ttcttcataa agatgaagct gcactacaaa aaattgatat tgaaaccatg tcatacatca 1981 agacaattaa cttgaaggac tataagtgcg ttcctcagtc attggcatat acacacttgg 2041 gaggctacta cttcattggc tgcaaacctg acagcaccgg agcagtttcc ccacaggtca 2101 tggtggacgg tgtaactgac tcagtcattg ggttcaatag tgatgtgacg ggcactccat 2161 atgtctctcc agatggccac taccttgtca gcattaatga tgtgaaaggt cttgtaaggg 2221 ttcagtacat taccatcaga ggagaaatac aggaggcttt tgatatttac acaaatctgc 2281 acatatctga tctggcattt caaccatcct ttactgaagc ccaccaatat aacatctacg 2341 gtagttcaag cacacaaact gatgtgctct ttgtggagct ctcttctggg aaggtcaaga 2401 tgataaagag tctcaaggaa ccactcaagg cagaagaatg gccttggaac cggaaaaaca 2461 ggcaaatcca ggacagtggc ttgtttggtc aatacctgat gacaccttcc aaggactctc 2521 tcttcatcct agatggacga ctcaataaat taaactgtga gatcactgaa gttgaaaaag 2581 gaaatacagt catttgggtt ggagatgcct tgccaacttt cttgtacaaa gtggttggta 2641 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2701 ggtggcgcgt taagtcgaca atcaacctct ggatta