Transcript: Mouse NM_178673.4

Mus musculus follistatin-like 5 (Fstl5), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Fstl5 (213262)
Length:
4820
CDS:
431..2974

Additional Resources:

NCBI RefSeq record:
NM_178673.4
NBCI Gene record:
Fstl5 (213262)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178673.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000053318 GCTAACATATTATGGAGAGAA pLKO.1 1745 CDS 100% 4.950 6.930 N FSTL5 n/a
2 TRCN0000200667 GCTAACATATTATGGAGAGAA pLKO.1 1745 CDS 100% 4.950 6.930 N Fstl5 n/a
3 TRCN0000215528 GAACTAACTCAGGTGATTAAA pLKO.1 1025 CDS 100% 15.000 12.000 N Fstl5 n/a
4 TRCN0000248114 GAACTAACTCAGGTGATTAAA pLKO_005 1025 CDS 100% 15.000 12.000 N Fstl5 n/a
5 TRCN0000248112 AGGAGCCCTCTTACCTAATTA pLKO_005 2974 CDS 100% 15.000 10.500 N Fstl5 n/a
6 TRCN0000257682 TGTCATGTGAAGGCATAATTT pLKO_005 3565 3UTR 100% 15.000 10.500 N Fstl5 n/a
7 TRCN0000248113 ACAGATCCTGTACCAGTTAAA pLKO_005 2048 CDS 100% 13.200 9.240 N Fstl5 n/a
8 TRCN0000189908 GCCTGCATGGACCTTTGTAAA pLKO.1 692 CDS 100% 13.200 9.240 N Fstl5 n/a
9 TRCN0000215796 CACATAAGCAATGTCCGTTAT pLKO.1 1625 CDS 100% 10.800 7.560 N Fstl5 n/a
10 TRCN0000053322 GCAGACAGTAATGGACTTGTA pLKO.1 995 CDS 100% 4.950 3.465 N FSTL5 n/a
11 TRCN0000191776 GCAATGAATCTACTGTCATTA pLKO.1 3483 3UTR 100% 1.320 0.924 N Fstl5 n/a
12 TRCN0000248111 CATCCACACCAACCTACATAT pLKO_005 2626 CDS 100% 13.200 7.920 N Fstl5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178673.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08651 pDONR223 100% 87.3% 91% None (many diffs) n/a
2 ccsbBroad304_08651 pLX_304 0% 87.3% 91% V5 (many diffs) n/a
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