Construct: ORF ccsbBroad304_08691

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000052.1_s304c1, BRDN0000402180
Derived from:
ccsbBroadEn_08691
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TWSG1 (57045)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08691
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57045 TWSG1 twisted gastrulation BMP si... NM_020648.6 99.8% 99.5% 21T>N
2 mouse 65960 Twsg1 twisted gastrulation BMP si... NM_023053.3 84.3% 93.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
735
ORF length:
669
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gttacactat gttgcngtgc ttactctagc catcctgatg ttcctgacat
121ggcttccaga atcactgagc tgtaacaaag cactctgtgc tagtgatgtg agcaaatgcc
181tcattcagga gctctgccag tgccggccgg gagaaggcaa ttgctcctgc tgtaaggagt
241gcatgctgtg tcttggggcc ctttgggacg agtgctgtga ctgtgttggt atgtgtaatc
301ctcgaaatta tagtgacaca cctccaactt caaagagcac agtggaggag ctgcatgaac
361cgatcccttc tctcttccgg gcactcacag aaggagatac tcagttgaat tggaacatcg
421tttctttccc tgttgcagaa gaactttcac atcatgagaa tctggtttca tttttagaaa
481ctgtgaacca gccacaccac cagaatgtgt ctgtccccag caataatgtt cacgcgcctt
541attccagtga caaagaacac atgtgtactg tggtttattt tgatgactgc atgtccatac
601atcagtgtaa aatatcctgt gagtccatgg gagcatccaa atatcgctgg tttcataatg
661cctgctgcga gtgcattggt ccagaatgta ttgactatgg tagtaaaact gtcaaatgta
721tgaactgcat gttttaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
781ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
841cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)