Construct: ORF ccsbBroad304_08736

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005751.1_s304c1, BRDN0000462054
Derived from:
ccsbBroadEn_08736
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRAMD1B (57476)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08736
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57476 GRAMD1B GRAM domain containing 1B NM_020716.3 99.9% 100% 930G>A;1425T>C
2 human 57476 GRAMD1B GRAM domain containing 1B XM_017018044.1 99.3% 99.4% 930G>A;1425T>C;1889_1890insGATCTGTTTCAG
3 human 57476 GRAMD1B GRAM domain containing 1B NM_001286563.2 98.9% 99% 235_255del;951G>A;1446T>C
4 human 57476 GRAMD1B GRAM domain containing 1B XM_017018043.1 96.4% 96.1% (many diffs)
5 human 57476 GRAMD1B GRAM domain containing 1B NM_001367419.1 96.1% 95.4% (many diffs)
6 human 57476 GRAMD1B GRAM domain containing 1B NM_001367418.1 95.6% 94.9% (many diffs)
7 human 57476 GRAMD1B GRAM domain containing 1B NM_001286564.2 94.4% 94.5% 0_1ins120;810G>A;1305T>C
8 human 57476 GRAMD1B GRAM domain containing 1B XM_017018047.1 94.4% 94.5% 0_1ins120;810G>A;1305T>C
9 human 57476 GRAMD1B GRAM domain containing 1B XM_017018048.1 94.4% 94.5% 0_1ins120;810G>A;1305T>C
10 human 57476 GRAMD1B GRAM domain containing 1B XM_005271619.4 94.3% 94% (many diffs)
11 human 57476 GRAMD1B GRAM domain containing 1B NM_001330396.2 93.9% 94% (many diffs)
12 human 57476 GRAMD1B GRAM domain containing 1B XM_017018050.1 93.9% 94% (many diffs)
13 human 57476 GRAMD1B GRAM domain containing 1B XM_011542929.2 93.7% 93.1% (many diffs)
14 human 57476 GRAMD1B GRAM domain containing 1B XM_024448622.1 93.5% 93% (many diffs)
15 human 57476 GRAMD1B GRAM domain containing 1B NM_001367420.1 93.2% 92.5% (many diffs)
16 human 57476 GRAMD1B GRAM domain containing 1B XM_011542933.1 92.7% 87.4% (many diffs)
17 human 57476 GRAMD1B GRAM domain containing 1B XM_017018040.1 91.6% 91.2% (many diffs)
18 human 57476 GRAMD1B GRAM domain containing 1B XM_005271624.4 91.6% 90.9% (many diffs)
19 human 57476 GRAMD1B GRAM domain containing 1B XM_017018039.1 90.8% 89.7% (many diffs)
20 human 57476 GRAMD1B GRAM domain containing 1B XM_011542926.2 89.1% 88.1% (many diffs)
21 human 57476 GRAMD1B GRAM domain containing 1B XM_005271621.4 89% 88.7% (many diffs)
22 human 57476 GRAMD1B GRAM domain containing 1B XM_005271623.4 89% 88.7% (many diffs)
23 human 57476 GRAMD1B GRAM domain containing 1B XM_011542930.2 89% 88.7% (many diffs)
24 human 57476 GRAMD1B GRAM domain containing 1B XM_011542931.2 89% 88.7% (many diffs)
25 human 57476 GRAMD1B GRAM domain containing 1B XM_017018045.1 89% 88.7% (many diffs)
26 human 57476 GRAMD1B GRAM domain containing 1B XM_017018046.1 89% 88.7% (many diffs)
27 human 57476 GRAMD1B GRAM domain containing 1B XM_006718892.3 89% 88% (many diffs)
28 human 57476 GRAMD1B GRAM domain containing 1B NM_001367421.2 87.3% 86.7% (many diffs)
29 human 57476 GRAMD1B GRAM domain containing 1B XM_017018051.1 78.5% 78.5% 0_1ins474;456G>A;951T>C
30 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510247.3 90.8% 97.2% (many diffs)
31 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313327.1 90.3% 96.7% (many diffs)
32 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510246.3 89.5% 95.4% (many diffs)
33 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510245.3 89% 95.3% (many diffs)
34 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313328.1 89% 94.9% (many diffs)
35 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313326.1 88.5% 94.8% (many diffs)
36 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510243.3 87.8% 93.5% (many diffs)
37 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510242.3 87.4% 92.3% (many diffs)
38 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510244.3 86.9% 92.2% (many diffs)
39 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313325.1 85.8% 91% (many diffs)
40 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510251.3 85.7% 91.8% (many diffs)
41 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510252.3 85.7% 91.8% (many diffs)
42 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510250.3 84% 84.2% (many diffs)
43 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510248.3 84% 90% (many diffs)
44 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510249.3 84% 90% (many diffs)
45 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313329.1 83.5% 83.7% (many diffs)
46 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510237.3 75.8% 80.4% (many diffs)
47 mouse 235283 Gramd1b GRAM domain containing 1B NM_172768.1 75.4% 80% (many diffs)
48 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510236.3 75.2% 79.8% (many diffs)
49 mouse 235283 Gramd1b GRAM domain containing 1B XM_006510235.3 74.7% 79.2% (many diffs)
50 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313319.1 74.6% 79.1% (many diffs)
51 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313320.1 74.6% 79.1% (many diffs)
52 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313321.1 74.6% 79.1% (many diffs)
53 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313322.1 74.6% 79.1% (many diffs)
54 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313323.1 74.1% 78.7% (many diffs)
55 mouse 235283 Gramd1b GRAM domain containing 1B XM_017313324.1 74.1% 78.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2283
ORF length:
2214
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaaaggattc aagctctcct gcactgccag taactccaac cgcagcacgc
121cggcctgctc gcccatcctc cggaagcggt ctcgctcgcc aaccccgcag aaccaggacg
181gagacaccat ggtggagaag ggctcagatc actcctcgga caagtccccg tccacaccgg
241agcagggcgt gcagcgcagc tgctcctccc agtccggccg gagcggcggc aagaattcca
301agaaaagcca gagttggtat aatgtgttaa gccccaccta caagcagaga aatgaagact
361tcagaaagct ctttaagcag cttccagaca cggagcgcct cattgttgat tactcatgtg
421cactccaaag agacattctc cttcagggcc gactctacct ctctgaaaat tggatctgct
481tctacagcaa catcttccgc tgggaaactc tgctgacagt ccgtttgaaa gacatctgtt
541ccatgactaa agaaaaaaca gctcgcctca ttcccaatgc catccaagtt tgcactgatt
601cagaaaagca cttcttcact tcgtttgggg cccgggatag gacatatatg atgatgttcc
661ggctctggca gaatgctctc cttgaaaagc ctctgtgtcc caaggagctc tggcactttg
721ttcaccagtg ctatgggaac gaattgggcc tgaccagtga tgacgaggac tacgtgcccc
781ctgacgacga cttcaacaca atgggatact gtgaagagat ccctgtggaa gagaatgaag
841tgaatgacag ctcatccaag agcagcatag agaccaagcc agatgccagt ccacagctgc
901ccaagaaatc catcaccaac agcacactaa catccacagg gagcagtgag gcccccgtct
961cgtttgatgg gctgcccctg gaggaagagg cgctggaagg agacgggtcc ctggaaaagg
1021agctcgccat tgacaacatc atgggggaga agattgagat gatcgctcct gtgaactccc
1081cttcactgga cttcaatgac aatgaggaca tccccactga gctcagtgac tcttccgaca
1141cacacgatga aggagaggtc caggccttct atgaggacct gagtggccgg cagtacgtga
1201atgaagtctt caacttcagc gtggacaagc tctatgacct cctcttcacc aactcgccct
1261tccagcggga tttcatggag cagcggcgct tctctgatat catcttccat ccatggaaaa
1321aggaggagaa tggaaaccag agccgagtga ttctttacac catcaccctt accaaccctc
1381tggctcccaa aactgccact gtcagggaga cacagaccat gtacaaggcg agccaggaga
1441gtgaatgtta cgtgatagat gccgaagtcc tcacccacga cgtgccctac cacgactact
1501tctacacaat caatcgctac acgctcaccc gtgtggctcg gaacaagagc cgactcaggg
1561tctccacaga gctgcgctat cgaaaacagc cctgggggtt agtgaaaacg ttcatcgaga
1621agaacttctg gagtgggctg gaggactact tccgccattt agagagcgag ctggccaaaa
1681cggagagcac ttatttggct gagatgcaca gacaatctcc caaagagaag gccagcaaga
1741ctacaacggt gcggaggagg aagcgtcccc atgcccacct gcgagtccct cacctggaag
1801aggtgatgag cccggtcacc acgcccacag atgaggatgt gggccacagg atcaaacatg
1861tggcaggttc cacacagacg cggcatatcc cggaggacac ccccaacggt ttccacctgc
1921agagcgtgtc caagctgctg ctggttatca gctgtgtgat ctgtttcagt ctggtgctgc
1981tggtcatcct taacatgatg ctcttctaca aactctggat gttggaatac accacgcaga
2041ccctcactgc ctggcagggt ctaaggctcc aagaaaggtt accccagtct cagacagaat
2101gggcccagct cttagagtcc caacaaaagt accacgatac tgagctccaa aaatggaggg
2161aaatcatcaa atcctcagtg atgctccttg accagatgaa ggactcgctc atcaaccttc
2221agaacggcat caggtcccgc gactacacgt cggaaagtga agaaaagagg aatcgctatc
2281atttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
2341tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2401tctggatta

Download FASTA (ORF) (Full)