Construct: ORF ccsbBroad304_08741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016143.1_s304c1, BRDN0000461694
- Derived from:
- ccsbBroadEn_08741
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUFIP2 (57532)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08741
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57532 | NUFIP2 | nuclear FMR1 interacting pr... | NM_020772.3 | 99.9% | 100% | 1335C>T |
| 2 | human | 57532 | NUFIP2 | nuclear FMR1 interacting pr... | XM_017024896.2 | 77% | 77.1% | 0_1ins477;858C>T |
| 3 | mouse | 68564 | Nufip2 | nuclear fragile X mental re... | NM_001024205.2 | 93.5% | 93.9% | (many diffs) |
| 4 | mouse | 68564 | Nufip2 | nuclear fragile X mental re... | XM_006534062.3 | 93.5% | 93.9% | (many diffs) |
| 5 | mouse | 68564 | Nufip2 | nuclear fragile X mental re... | XM_006534063.3 | 71.6% | 72% | (many diffs) |
| 6 | mouse | 68564 | Nufip2 | nuclear fragile X mental re... | XM_017314730.1 | 71.6% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2154
- ORF length:
- 2085
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggagaag cccggccagc cacagcctca gcaccatcac agccaccacc 121 atccgcacca tcaccctcag cagcagcagc agcagccgca ccaccaccac cattattatt 181 tctacaacca cagccacaac caccaccacc accatcatca ccagcagcct caccaatacc 241 tgcagcatgg agccgagggc agccccaagg cccagccaaa gccgctgaaa catgagcaga 301 aacacaccct ccagcagcac caggaaacgc cgaagaagaa aacaggctat ggtgaactaa 361 acggtaatgc tggagaaaga gaaatatctt taaagaacct gagttctgat gaagccacca 421 accctatttc cagggtcctc aatggcaacc agcaagttgt agacactagc ctgaagcaga 481 ctgtaaaggc caacaccttt gggaaagcag gaattaaaac caagaatttc attcagaaaa 541 acagtatgga caaaaagaat gggaagtctt atgaaaataa atctggagag aatcagtctg 601 tagataagtc tgatactata ccaattccaa atggtgtggt aacaaataat tctggttata 661 ttactaatgg ttatatgggt aaaggagcag ataatgatgg tagtggatct gagagcggat 721 atacaactcc taaaaaaagg aaagctaggc gcaatagtgc caagggttgt gaaaacctta 781 atatagtgca ggacaaaata atgcaacaag agaccagtgt cccaacctta aaacagggac 841 ttgaaacttt caagcctgac tatagtgaac aaaagggaaa tcgagtagat ggttcgaagc 901 ccatttggaa gtatgaaact gggcctggag gaacaagtcg aggaaaacct gctgtgggtg 961 atatgcttcg gaaaagctca gatagtaaac ctggtgtgag cagcaaaaag tttgatgatc 1021 ggcccaaagg aaagcatgct tcagctgttg cctccaaaga ggactcgtgg accctattta 1081 aaccaccccc agtttttcca gtggacaata gcagtgctaa aatagttcct aaaataagtt 1141 atgcaagcaa agttaaggaa aacctcaaca aaactataca gaactcttct gtgtcaccaa 1201 cttcatcttc atcatcttca tcatctaccg gggaaactca gacccaatca tcaagtcgct 1261 tatcccaggt ccctatgtca gcgctgaaat ctgttacttc tgccaacttt tctaatgggc 1321 ctgttttagc agggactgat ggaaatgttt atcctccagg gggtcagcca ctgctaacta 1381 ctgctgctaa tactctaaca cctatctctt ctgggacaga ttcagttctc caggacatga 1441 gtctaacttc agcagctgtt gaacaaatta agactagcct ttttatctat ccttcaaata 1501 tgcaaactat gctgttgagc acagcacaag tggatctgcc ctctcagaca gatcagcaaa 1561 acctggggga tatcttccag aatcagtggg gtttatcatt tataaatgag cccagtgctg 1621 gccctgagac tgttactggg aagtcatcag agcataaagt gatggaggtg acatttcaag 1681 gagaatatcc tgctactttg gtttcacagg gtgctgaaat aattccctca ggaactgagc 1741 atcctgtgtt tcccaaggct tacgagctgg agaaacggac tagtcctcaa gttctgggta 1801 gcattctaaa atctgggact actagtgaga gtggagcctt atccttggaa cccagtcata 1861 taggtgacct gcagaaagca gacaccagta gtcaaggtgc tttagtgttt ctctcaaagg 1921 actacgagat agaaagtcaa aatcctctgg cctctcctac gaacactttg ttaggctctg 1981 ccaaagaaca gagataccag agaggcctag aaaggaatga tagctggggt tcttttgacc 2041 tgagggctgc tattgtatat cacactaaag aaatggaatc tatttggaat ttgcagaagc 2101 aagatcccaa aaggataatc acttacaatg aagccatgga tagtccagat caattgccaa 2161 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2221 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta