Construct: ORF ccsbBroad304_08755

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004002.1_s304c1, BRDN0000401998
Derived from:
ccsbBroadEn_08755
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BEND3 (57673)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08755
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57673 BEND3 BEN domain containing 3 NM_001080450.2 99.9% 99.8% 1787A>G
2 human 57673 BEND3 BEN domain containing 3 NM_001367314.1 99.9% 99.8% 1787A>G
3 human 57673 BEND3 BEN domain containing 3 XM_005267080.4 99.9% 99.8% 1787A>G
4 human 57673 BEND3 BEN domain containing 3 XM_011536005.3 99.9% 99.8% 1787A>G
5 human 650623 BEND3P3 BEN domain containing 3 pse... NR_027512.1 34.8% (many diffs)
6 mouse 331623 Bend3 BEN domain containing 3 NM_199028.2 86.1% 90.7% (many diffs)
7 mouse 331623 Bend3 BEN domain containing 3 XM_006512769.3 86.1% 90.7% (many diffs)
8 mouse 331623 Bend3 BEN domain containing 3 XM_006512770.3 86.1% 90.7% (many diffs)
9 mouse 331623 Bend3 BEN domain containing 3 XM_006512771.3 86.1% 90.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2553
ORF length:
2484
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaactcaact gaattcaccg aagatgtaga agaagttcta aaaagtatca
121ctgtgaaagt ggagacagag gctgaagatg ctgctctgga ctgctccgtg aattccagga
181cttctgagaa gcactctgtg gacagcgtcc tcactgccct gcaggactcc agcaaacgaa
241agcagctggt cagcgatggc ctgctagact ctgtccccgg cgtgaagagg aggcggctga
301tccccgaggc tctcctagca ggcatgcgga accgtgagaa cagctcgccc tgccaaggca
361atggtgagca ggccggcagg ggcaggagcc tgggcaatgt gtggcctgga gaggaggagc
421cctgcaacga tgccaccacc ccttcctaca agaagcctct gtatggcatc tcgcacaaga
481tcatggagaa gaagaatcct ccctcggggg acctgctaaa cgtgtacgag ctctttgaga
541aggcaaacgc cagcaacagc ccctcgtcac tgcggctcct gaatgagcca cagaagcggg
601actgtggcag caccggggca ggcactgaca acgaccccaa catctacttc ctgatccaga
661agatgttcta catgctgaac accctcacgt ccaacatgtc ccagctgcac agcaaggtgg
721acctgctctc ccttgaggtg agccgcatca agaagcaggt gagccccact gagatggtgg
781ccaaattcca gccgccccct gagtaccagc tcacagccgc agagctcaag cagatcgtgg
841accagagcct gtcagggggg gacctggcct gccgcttgct ggtgcagctc ttccccgagc
901tcttcagcga cgtggacttc tcccggggct gcagtgcctg tggctttgcg gccaagcgca
961agctggagtc gctgcacctg cagctcatcc gcaactatgt ggaggtctac tacccctcgg
1021tgaaggacac ggctgtgtgg caggccgagt gcctgcccca gctgaacgac ttcttcagcc
1081gcttctgggc ccagcgggaa atggaggaca gccagcccag cggccaggtc gccagcttct
1141ttgaggcaga gcaggtggac cccggccact tcctggacaa caaagaccag gaggaggccc
1201tgtctcttga ccggagcagc accatcgcct cagaccacgt ggtggacacg caggacctca
1261ctgagttcct ggacgaagcc tcctcaccag gcgagtttgc cgtcttcctc ctccaccggc
1321tcttccccga gctcttcgac caccgcaagc tgggtgaaca gtacagctgc tacggggacg
1381gtggaaagca ggagctggac ccgcagcggc tgcagatcat ccgcaactac acggagatct
1441acttccctga catgcaggag gaggaggcct ggctgcagca gtgtgcccag cgcatcaacg
1501acgagctcga gggcctgggg ctggacgcgg gcagtgaagg cgaccccccg cgtgatgact
1561gctacgactc ctccagtctg cccgacgaca tctcagtggt caaggtggag gacagcttcg
1621agggcgagcg gccgggtcgc cgctccaaga agatctggct ggtgcccatc gacttcgaca
1681agttagagat cccccagcct gacttcgagg tgcccggtgc cgactgcctg ctcagcaagg
1741agcagctacg cagcatctac gagagcagcc tgtccatcgg caacttcgcc tcgcgcctgc
1801tggtgcacct gttccccgag ctcttcacgc acgagaacct gcgcaagcag tacagctgca
1861gcggctccct gggcaagaag cagctggacc cctcccgcat caagctcatc cgccactacg
1921tgcagctgct ctacccacgc gccaaaaacg accgcgtctg gaccctggag ttcgtgggca
1981aactggatga gcgctgccgg cgccgggaca cggagcaaag gcgctcctac cagcagcagc
2041gcaaggtcca cgtgccgggc cctgagtgca gagacttgac cagctatgca atcaaccccg
2101agaggttccg ggaggagttt gaggggcccc cactgccccc cgagaggagc agcaaggact
2161tttgcaagat ccccttggac gagctggtgg tcccctcgcc tgacttcccg gtgccttctc
2221cctacctgct gtctgacaag gaggtgcgtg agatcgtgca gcagagcctc tccgtgggca
2281actttgccgc ccggctcctc gtccgcctgt ttcccgaact cttcaccgcc gagaacctcc
2341ggctgcagta caaccattcc ggggcttgca acaagaagca actggacccc acgcggctgc
2401ggctcatccg ccactacgtg gaagccgtct acccggtgga gaagatggag gaggtgtggc
2461actacgaatg tatccccagc atcgatgaga ggtgccgccg ccccaacagg aaaaaatgcg
2521acatcctcaa gaaagcaaag aaagtggaga agttgccaac tttcttgtac aaagtggttg
2581gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2641accggtggcg cgttaagtcg acaatcaacc tctggatta

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