Construct: ORF ccsbBroad304_08896

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004115.1_s304c1, BRDN0000400218
Derived from:
ccsbBroadEn_08896
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CYP4F12 (66002)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08896
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 66002 CYP4F12 cytochrome P450 family 4 su... NM_023944.4 99.8% 99.4% 47C>T;226A>G;562C>T
2 human 57834 CYP4F11 cytochrome P450 family 4 su... NM_001128932.1 86.8% 82.8% (many diffs)
3 human 57834 CYP4F11 cytochrome P450 family 4 su... NM_021187.3 86.8% 82.8% (many diffs)
4 human 4051 CYP4F3 cytochrome P450 family 4 su... NM_001199208.2 86% 80.7% (many diffs)
5 human 4051 CYP4F3 cytochrome P450 family 4 su... NM_001199209.2 86% 80.7% (many diffs)
6 human 4051 CYP4F3 cytochrome P450 family 4 su... NM_001369696.1 86% 80.7% (many diffs)
7 human 8529 CYP4F2 cytochrome P450 family 4 su... NM_001082.5 85.9% 81.2% (many diffs)
8 human 4051 CYP4F3 cytochrome P450 family 4 su... NM_000896.3 81.9% 77.6% (many diffs)
9 human 66002 CYP4F12 cytochrome P450 family 4 su... XM_017027172.1 78.2% 70% (many diffs)
10 human 66002 CYP4F12 cytochrome P450 family 4 su... NR_117085.2 74.2% (many diffs)
11 human 66002 CYP4F12 cytochrome P450 family 4 su... XM_006722850.3 71.5% 71.3% 0_1ins447;115C>T
12 human 66002 CYP4F12 cytochrome P450 family 4 su... XM_011528202.3 71.5% 71.3% 0_1ins447;115C>T
13 human 66002 CYP4F12 cytochrome P450 family 4 su... XM_011528208.2 71.5% 71.3% 0_1ins447;115C>T
14 human 11283 CYP4F8 cytochrome P450 family 4 su... XM_024451341.1 67.9% 62.5% (many diffs)
15 human 66002 CYP4F12 cytochrome P450 family 4 su... XM_011528203.2 66.6% 66.4% (many diffs)
16 human 66002 CYP4F12 cytochrome P450 family 4 su... XM_011528204.2 66.6% 66.4% (many diffs)
17 human 66002 CYP4F12 cytochrome P450 family 4 su... XM_011528205.2 66.6% 66.4% (many diffs)
18 human 66002 CYP4F12 cytochrome P450 family 4 su... XM_011528207.2 63.7% 63.7% 0_1ins570
19 human 4051 CYP4F3 cytochrome P450 family 4 su... XM_017026815.1 63.2% 57.2% (many diffs)
20 human 4051 CYP4F3 cytochrome P450 family 4 su... XM_005259911.5 62.4% 60.3% (many diffs)
21 human 4051 CYP4F3 cytochrome P450 family 4 su... XM_011528014.3 62.4% 60.3% (many diffs)
22 human 388514 CYP4F24P cytochrome P450 family 4 su... NR_033864.1 40% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1638
ORF length:
1572
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc gctgctgagc ctgccctggc tgggcctcag accggtggca atgtccccat
121ggctactcct gctgctggtt gtgggctcct ggctactcgc ccgcatcctg gcttggacct
181atgccttcta taacaactgc cgccggctcc agtgtttccc acagccccca aaacggaact
241ggttttgggg tcacctgggc ctgatcactc ctacagagga gggcttgaag gactcgaccc
301agatgtcggc cacctattcc cagggcttta cggtatggct gggtcccatc atccccttca
361tcgttttatg ccaccctgac accatccggt ctatcaccaa tgcctcagct gccattgcac
421ccaaggataa tctcttcatc aggttcctga agccctggct gggagaaggg atactgctga
481gtggcggtga caagtggagc cgccaccgtc ggatgctgac gcccgccttc catttcaaca
541tcctgaagtc ctatataacg atcttcaaca agagtgcaaa catcatgctt gacaagtggc
601agcacctggc ctcagagggc agcagttgtc tggacatgtt tgagcacatc agcctcatga
661ccttggacag tctacagaaa tgcatcttca gctttgacag ccattgtcag gagaggccca
721gtgaatatat tgccaccatc ttggagctca gtgcccttgt agagaaaaga agccagcata
781tcctccagca catggacttt ctgtattacc tctcccatga cgggcggcgc ttccacaggg
841cctgccgcct ggtgcatgac ttcacagacg ctgtcatccg ggagcggcgt cgcaccctcc
901ccactcaggg tattgatgat tttttcaaag acaaagccaa gtccaagact ttggatttca
961ttgatgtgct tctgctgagc aaggatgaag atgggaaggc attgtcagat gaggatataa
1021gagcagaggc tgacaccttc atgtttggag gccatgacac cacggccagt ggcctctcct
1081gggtcctgta caaccttgcg aggcacccag aataccagga gcgctgccga caggaggtgc
1141aagagcttct gaaggaccgc gatcctaaag agattgaatg ggacgacctg gcccagctgc
1201ccttcctgac catgtgcgtg aaggagagcc tgaggttaca tcccccagct cccttcatct
1261cccgatgctg cacccaggac attgttctcc cagatggccg agtcatcccc aaaggcatta
1321cctgcctcat cgatattata ggggtccatc acaacccaac tgtgtggccg gatcctgagg
1381tctacgaccc cttccgcttt gacccagaga acagcaaggg gaggtcacct ctggctttta
1441ttcctttctc cgcagggccc aggaactgca tcgggcaggc gttcgccatg gcggagatga
1501aagtggtcct ggcgttgatg ctgctgcact tccggttcct gccagaccac actgagcccc
1561gcaggaagct ggaattgatc atgcgcgccg agggcgggct ttggctgcgg gtggagcccc
1621tgaatgtaag cttgcagagc ccaactttct tgtacaaagt ggttggtaag cctatcccta
1681accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1741agtcgacaat caacctctgg atta

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