Transcript: Human NM_001199208.2

Homo sapiens cytochrome P450 family 4 subfamily F member 3 (CYP4F3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
CYP4F3 (4051)
Length:
5053
CDS:
51..1613

Additional Resources:

NCBI RefSeq record:
NM_001199208.2
NBCI Gene record:
CYP4F3 (4051)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001199208.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000064561 CCCGAAACGGAATTGGTTCTT pLKO.1 212 CDS 100% 4.950 6.930 N CYP4F3 n/a
2 TRCN0000422526 AGTGCCCGTCTGGACATGTTT pLKO_005 606 CDS 100% 5.625 3.938 N CYP4F3 n/a
3 TRCN0000064559 GTACATAGACTTCCTGTATTA pLKO.1 773 CDS 100% 13.200 7.920 N CYP4F3 n/a
4 TRCN0000434011 CCAAGACATTGTGCTCCCAGA pLKO_005 1259 CDS 100% 2.160 1.296 N CYP4F3 n/a
5 TRCN0000431676 AGCAGAAGCTGACACCTTTAT pLKO_005 1007 CDS 100% 13.200 6.600 Y CYP4F2 n/a
6 TRCN0000433806 CCAAGACTTTGGACTTCATTG pLKO_005 928 CDS 100% 10.800 5.400 Y CYP4F2 n/a
7 TRCN0000429359 ATCATCCGGTCTGTCATCAAC pLKO_005 366 CDS 100% 4.950 2.475 Y CYP4F2 n/a
8 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 4238 3UTR 100% 4.950 2.475 Y CFLAR n/a
9 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 4238 3UTR 100% 4.950 2.475 Y C19orf31 n/a
10 TRCN0000064401 CCAGGGCTTTAAGGTCTGGAT pLKO.1 305 CDS 100% 2.640 1.320 Y CYP4F2 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3202 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 4236 3UTR 100% 4.950 2.475 Y ERN2 n/a
13 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 4236 3UTR 100% 4.950 2.475 Y P3H4 n/a
14 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 4236 3UTR 100% 4.950 2.475 Y P3H4 n/a
15 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3202 3UTR 100% 5.625 2.813 Y EID2B n/a
16 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 4403 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001199208.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07253 pDONR223 100% 94.5% 92.6% None (many diffs) n/a
2 ccsbBroad304_07253 pLX_304 0% 94.5% 92.6% V5 (many diffs) n/a
3 TRCN0000477423 CCTAGGAGTTGTCCATAAAGAAGC pLX_317 25% 94.5% 92.6% V5 (many diffs) n/a
4 ccsbBroadEn_08771 pDONR223 100% 88.5% 85.8% None (many diffs) n/a
5 ccsbBroad304_08771 pLX_304 0% 88.5% 85.8% V5 (many diffs) n/a
6 TRCN0000481046 ATCCATTACTGGGAGATGTCCCCC pLX_317 25.3% 88.5% 85.8% V5 (many diffs) n/a
7 ccsbBroadEn_08896 pDONR223 100% 86% 80.7% None (many diffs) n/a
8 ccsbBroad304_08896 pLX_304 0% 86% 80.7% V5 (many diffs) n/a
9 TRCN0000467927 CCTCCCATATTCTGTTCTGTTGAC pLX_317 28% 86% 80.7% V5 (many diffs) n/a
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