Construct: ORF ccsbBroad304_09005

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000446.1_s304c1, BRDN0000404865
Derived from:
ccsbBroadEn_09005
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCDC170 (80129)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09005
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80129 CCDC170 coiled-coil domain containi... NM_025059.4 99.8% 99.7% 991G>A;1317T>C;2047G>A
2 human 80129 CCDC170 coiled-coil domain containi... XM_011536147.2 97.5% 96.8% (many diffs)
3 human 80129 CCDC170 coiled-coil domain containi... XM_011536148.2 88.2% 87.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2211
ORF length:
2145
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag cctggactgc accagccata tcgcgctggg tgccgcttcg ccagcgcccg
121aggaaactta cgatcatctt tcggaagtcc cggtcacgcg ggagcagtta aaccactatc
181ggaatgtggc tcaaaatgct cgaagtgaac ttgcagcaac tttggtcaaa tttgaatgtg
241ctcagtctga gcttcaagac ctccgatcca agatgctttc taaagaagtc tcctgtcaag
301aactgaaagc tgaaatggag agctacaagg aaaacaatgc cagaaaatca tctctcctta
361cctctttgag agacagagtt caggaactag aagaagaatc agcagcactt tccacttcta
421aaatcagaac agaaatcaca gctcacgctg caatcaagga gaaccaggaa ttaaagaaga
481aagttgtaga gttaaatgaa aaattacaaa agtgttcaaa agaaaatgag gagaataaga
541aacaagtttc aaagaattgc aggaaacatg aggaatttct gactcaactg cgtgactgct
601tggatccaga tgagaggaat gacaaggcat cagatgaaga tttaatttta aagcttagag
661acctgcgcaa agaaaatgaa ttcgtgaaag gacaaattgt tattcttgaa gagactataa
721atgtccatga gatggaagca aaagctagca gagaaacgat catgaggctg gcttcagaag
781tcaacagaga gcagaaaaaa gctgcctcct gtactgaaga gaaagagaag ctgaaccagg
841acctgctcag tgctgtagaa gcaaaagaag ctcttgaaag ggaagttaag atcttccaag
901aaaggctgct tgctggccag caggtctggg atgcctcaaa gcaggaagtg agcctcctga
961agaaaagctc ttctgagttg gagaagagtt tgaaggccag tcaggatgca gtcacaacct
1021cacaaagcca gtacttctca tttagggaga aaatcacagc cctccttagg ggcagattga
1081gcatgactgg gtccactgag gacaccattt tggagaagat tcgagaaatg gacagccggg
1141aagaaagcag ggaccggatg gtctcccagc ttgaagccca aatatctgag cttgttgaac
1201agttgggaaa ggagtctggg tttcaccaga aagctctcca gagggcccag aaagcagaga
1261atatgttgga gactcttcag ggtcagctga cacacctgga ggcagagctg gtttctggag
1321gtgttttgcg agacaacttg aattttgaga aacaaaaata tcttaaattt ctggatcagc
1381tctctcagaa aatgaagttg gaccagatgg ctgccgaact tggctttgac atgcggctgg
1441acgtggtttt agctcgaaca gagcagctgg ttcgtcttga gagcaatgca gtcattgaga
1501acaagaccat tgcccacaat ttgcagagaa agctaaagac acagaaagag agactggaga
1561gcaaagaatt acacatgagc ctcctccggc agaaaatagc ccagctggag gaggagaagc
1621aggcacgcac ggccttggtg gttgagaggg acaacgcgca tcttaccatc aggaacttgc
1681agaagaaggt ggagaggctg cagaaagagc tgaacacgtg tcgagacttg cacaccgagc
1741tcaaagccaa actggccgac accaatgaac tgaagattaa aactttggaa cagactaaag
1801ccattgaaga tctaaacaaa tccagagacc aactggagaa gatgaaggag aaagctgaga
1861aaaagctcat gtctgtcaag tcagaactgg ataccacaga acatgaggct aaggagaata
1921aagaaagggc cagaaacatg atagaagtgg taaccagtga aatgaagaca ctaaaaaaat
1981ctctggaaga agcagaaaag agagaaaagc agctggcaga cttcagggag gtggtgtcgc
2041agatgctagg cttgaacgtg accagccttg ctcttcctga ttatgaaatc atcaagtgtc
2101ttgaaagatt gatccattca catcagcatc actttgttac ctgtgcctgc ctcaaagatg
2161tgactactgg gcaagagagg cacccacaag gccatttaca gcttcttcat tgcccaactt
2221tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
2281agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

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