Construct: ORF ccsbBroad304_09013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006543.1_s304c1, BRDN0000405056
- Derived from:
- ccsbBroadEn_09013
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OPA3 (80207)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09013
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80207 | OPA3 | outer mitochondrial membran... | NM_025136.4 | 99.8% | 100% | 231T>C |
| 2 | human | 80207 | OPA3 | outer mitochondrial membran... | NM_001017989.3 | 84.9% | 77.3% | (many diffs) |
| 3 | human | 80207 | OPA3 | outer mitochondrial membran... | XM_006723403.4 | 70.2% | 70.3% | 0_1ins159;72T>C |
| 4 | mouse | 403187 | Opa3 | optic atrophy 3 | NM_207525.3 | 82.4% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 61
- ORF end:
- 598
- ORF length:
- 537
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 atggtggtgg gcgcgttccc tatggcgaag ctgctatact tgggcatccg gcaggtcagc 121 aagccgcttg ccaaccgtat taaggaggcc gcccgccgaa gcgagttctt caagacctat 181 atctgcctcc cgccggctca actgtatcac tgggtggaga tgcggaccaa gatgcgcatc 241 atgggcttcc ggggcacggt catcaagccg ctgaacgagg aggcggcagc cgagctgggc 301 gcagagctgc tgggcgaagc caccatcttc atcgtgggcg gcggctgcct agtgctggag 361 tactggcgcc accaggcgca gcagcgccac aaggaggagg agcagcgtgc tgcctggaac 421 gcgctgcggg acgaggtggg ccacctggcg ctggcgctgg aagcgctgca ggcgcaggtg 481 caggcggcgc cgccacaggg cgccctggag gaactgcgca cagagctgca agaggtgcgc 541 gcccagctct gcaatcccgg ccggtccgct tcccacgcag tgcctgcgtc caagaaatag 601 ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat 661 tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg 721 atta