Construct: ORF ccsbBroad304_09028

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013122.1_s304c1, BRDN0000407119
Derived from:
ccsbBroadEn_09028
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CEP70 (80321)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09028
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80321 CEP70 centrosomal protein 70 NM_001320598.2 99.8% 98.6% 404G>A;462A>G;1771A>T
2 human 80321 CEP70 centrosomal protein 70 NM_024491.4 99.8% 98.6% 404G>A;462A>G;1771A>T
3 human 80321 CEP70 centrosomal protein 70 XM_017007275.1 99.8% 98.6% 404G>A;462A>G;1771A>T
4 human 80321 CEP70 centrosomal protein 70 NM_001288964.2 96.8% 95.6% (many diffs)
5 human 80321 CEP70 centrosomal protein 70 NM_001288965.2 96.4% 95.3% (many diffs)
6 human 80321 CEP70 centrosomal protein 70 NM_001320599.2 96.2% 95.6% (many diffs)
7 human 80321 CEP70 centrosomal protein 70 XM_024453781.1 96.2% 95.6% (many diffs)
8 human 80321 CEP70 centrosomal protein 70 XM_017007271.1 93.3% 91.6% (many diffs)
9 human 80321 CEP70 centrosomal protein 70 XM_017007272.1 93.3% 91.6% (many diffs)
10 human 80321 CEP70 centrosomal protein 70 XM_024453780.1 93.3% 91.6% (many diffs)
11 human 80321 CEP70 centrosomal protein 70 NM_001288966.1 92.6% 93% (many diffs)
12 human 80321 CEP70 centrosomal protein 70 XM_017007273.1 90.1% 88.4% (many diffs)
13 human 80321 CEP70 centrosomal protein 70 XM_017007274.1 90.1% 88.4% (many diffs)
14 human 80321 CEP70 centrosomal protein 70 XM_017007276.2 74.6% 72.9% (many diffs)
15 human 80321 CEP70 centrosomal protein 70 NM_001288967.2 74.3% 72.8% (many diffs)
16 human 80321 CEP70 centrosomal protein 70 XM_011513209.3 69.6% 68.6% (many diffs)
17 human 80321 CEP70 centrosomal protein 70 XM_017007277.1 69.2% 67.2% (many diffs)
18 human 80321 CEP70 centrosomal protein 70 XM_017007278.2 65.6% 63.9% (many diffs)
19 human 80321 CEP70 centrosomal protein 70 XM_017007279.1 57.6% 55.3% (many diffs)
20 human 80321 CEP70 centrosomal protein 70 XM_017007282.2 56.5% 55.4% 0_1ins777;994A>T
21 human 80321 CEP70 centrosomal protein 70 XM_017007283.1 56.5% 55.4% 0_1ins777;994A>T
22 human 80321 CEP70 centrosomal protein 70 XM_017007284.1 56.5% 55.4% 0_1ins777;994A>T
23 human 80321 CEP70 centrosomal protein 70 XM_017007280.2 52.5% 50.7% (many diffs)
24 human 80321 CEP70 centrosomal protein 70 XM_017007281.2 52.5% 50.7% (many diffs)
25 human 80321 CEP70 centrosomal protein 70 NM_001320600.1 35.3% 35.7% 404G>A;462A>G;636_637ins1155
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1836
ORF length:
1770
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt tccggtagcc cctaaacccc aggattccag tcaaccatca gacagactca
121tgactgaaaa acagcaggaa gaagcagaat gggaaagcat aaatgtgcta ttgatgatgc
181atggcttaaa acctttgtct ctagtcaaaa gaacagatct caaagatctc atcatttttg
241acaaacagtc atcacaaagg atgagacaga atttgaaatt gttggtggaa gaaacatcat
301gtcaacagaa catgatacag gagcttatag aaactaatca acagcttaga aatgaacttc
361agctagagca aagccgagca gccaatcaag aacaacgagc taatgacttg gaacaaatta
421tggaaagtgt gaaatccaaa attggtgaat tggaggatga atcactaaat agggcttgcc
481accaacagaa taaaataaaa gatcttcaaa aggagcagaa aactttgcag gtgaagtgcc
541agcattataa gaaaaaacga acggagcaag aagaaactat tgcttctttg caaatggaag
601tctgtagatt aaaaaaggag gaagaagatc gcattgtcac tcaaaacaga gtgtttgcct
661atctgtgcaa aagagttcct cataccgtct tggatagaca gttgctttgt ctaattgatt
721actatgaatc taaaattaga aaaattcata cacaaaggca atataaagaa gatgaaagtc
781agtcagaaga agaaaacgac tacagaaatc tggatgcctc accaacttat aaaggccttt
841taatgtcatt acagaatcaa ctcaaggaat caaaatctaa gattgatgca ctttcaagtg
901aaaagctgaa cctccaaaaa gatttggaaa ccaggcctac gcagcatgaa ttaagacttt
961ataaacagca ggtgaagaag ctggaaaaag cccttaagaa aaacgtcaaa ttacaggagc
1021ttattaatca taagaaggct gaggacacag agaagaaaga tgagcccagc aaatataatc
1081agcaacaggc cctaattgac cagagatact ttcaggtgct gtgtagcatc aattcaatta
1141tccacaatcc aagagctcca gtaataattt ataaacagac caaaggggga gtccaaaatt
1201ttaataaaga tcttgttcaa gattgtggat ttgagcatct tgttcctgta atagaaatgt
1261gggcagatca actgacatcc ttaaaggatt tgtataagtc cttgaaaaca ctatctgcag
1321aactggtacc ttggcttaat ttgaagaagc aggatgaaaa tgaaggtatc aaagttgaag
1381atttgttgtt tatagtagat actatgctgg aagaagttga aaataaggaa aaggacagca
1441atatgccaca ctttcaaact ttgcaagcta ttgtttctca cttccaaaag ttatttgatg
1501tgccttcttt aaatggagtc tatccccgaa tgaatgaagt ttatactagg cttggagaaa
1561tgaacaatgc tgtgagaaac ctccaagaac tcttagaatt agatagttca tcctcattgt
1621gtgtgctagt aagcactgtt ggaaaactct gtaggctgat taatgaagat gtgaatgagc
1681aggttatgca ggtattagga cctgaagacc tccagagcat tatctacaaa ttggaagaac
1741acgaggaatt tttcccagca tttcaggcat ttactaatga tctacttgaa atcttagaaa
1801ttgatgactt ggatgccatt gtacctgcag taaagtaatt aaaagtactt tcatactgcc
1861caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1921ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1981tta

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