Construct: ORF ccsbBroad304_09147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018063.1_s304c1, BRDN0000408498
- Derived from:
- ccsbBroadEn_09147
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB6C (84084)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09147
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84084 | RAB6C | RAB6C, member RAS oncogene ... | NM_032144.3 | 99.8% | 99.6% | 475G>A |
| 2 | human | 150786 | RAB6D | RAB6D, member RAS oncogene ... | NM_001077637.1 | 99.3% | 98.4% | (many diffs) |
| 3 | human | 5870 | RAB6A | RAB6A, member RAS oncogene ... | NM_002869.5 | 78.7% | 74.4% | (many diffs) |
| 4 | human | 5870 | RAB6A | RAB6A, member RAS oncogene ... | NM_198896.2 | 75% | 73.2% | (many diffs) |
| 5 | human | 5870 | RAB6A | RAB6A, member RAS oncogene ... | NM_001243719.1 | 66% | 62.2% | (many diffs) |
| 6 | human | 5870 | RAB6A | RAB6A, member RAS oncogene ... | NM_001243718.2 | 39.3% | 37.7% | (many diffs) |
| 7 | mouse | 19346 | Rab6a | RAB6A, member RAS oncogene ... | NM_001163663.1 | 75.7% | 74.4% | (many diffs) |
| 8 | mouse | 19346 | Rab6a | RAB6A, member RAS oncogene ... | NM_024287.4 | 72.3% | 73.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 831
- ORF length:
- 762
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccgcgggc ggagacttcg ggaatccgct gaggaaattc aagctggtgt 121 tcctggggga gcaaagcgtt gcaaagacat ctttgatcac cagattcagg tatgacagtt 181 ttgacaacac ctatcaggca ataattggca ttgacttttt atcaaaaact atgtacttgg 241 aggatggaac aatcgggctt cggctgtggg atacggcggg tcaggaacgt ctccgtagcc 301 tcattcccag gtacatccgt gattctgctg cagctgtagt agtttacgat atcacaaatg 361 ttaactcatt ccagcaaact acaaagtgga ttgatgatgt cagaacagaa agaggaagtg 421 atgttatcat cacgctagta ggaaatagaa cagatcttgc tgacaagagg caagtgtcag 481 ttgaggaggg agagaggaaa gccaaagggc tgaatgttac gtttattgaa actagggcaa 541 aaactggata caatgtaaag cagctctttc gacgtgtagc agcagctttg ccgggaatgg 601 aaagcacaca ggacggaagc agagaagaca tgagtgacat aaaactggaa aagcctcagg 661 agcaaacagt cagcgaaggg ggttgttcct gctactctcc catgtcatct tcaacccttc 721 ctcagaagcc cccttactct ttcattgact gcagtgtgaa tattggcttg aaccttttcc 781 cttcattaat aacgttttgc aattcatcat tgctgcctgt ctcgtggagg ttgccaactt 841 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 901 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta