Construct: ORF ccsbBroad304_09216
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006261.1_s304c1, BRDN0000400131
- Derived from:
- ccsbBroadEn_09216
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACCS (84680)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09216
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | NM_001127219.2 | 99.8% | 99.8% | 871C>T;1262C>T |
| 2 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | NM_032592.4 | 99.8% | 99.8% | 871C>T;1262C>T |
| 3 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_024448718.1 | 85.3% | 81% | 871C>T;1253_1254ins154;1350_1425del |
| 4 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_024448719.1 | 85.3% | 81% | 871C>T;1253_1254ins154;1350_1425del |
| 5 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_005253170.3 | 83.1% | 83% | 1_303del;1174C>T;1565C>T |
| 6 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_011520407.1 | 75.7% | 75.6% | 0_1ins363;508C>T;899C>T |
| 7 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_024448720.1 | 75.7% | 75.6% | 0_1ins363;508C>T;899C>T |
| 8 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_005253172.2 | 73.9% | 72.2% | (many diffs) |
| 9 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_011520406.2 | 71.6% | 68% | (many diffs) |
| 10 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_930915.2 | 65.3% | (many diffs) | |
| 11 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_006718348.2 | 63.5% | 59.6% | (many diffs) |
| 12 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_024448721.1 | 63.5% | 59.6% | (many diffs) |
| 13 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_011520410.2 | 52.9% | 52.8% | 0_1ins705;166C>T;557C>T |
| 14 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_024448723.1 | 52.9% | 52.8% | 0_1ins705;166C>T;557C>T |
| 15 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_001748003.1 | 46.7% | (many diffs) | |
| 16 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_001748004.1 | 42.6% | (many diffs) | |
| 17 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_001748007.1 | 41.4% | (many diffs) | |
| 18 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_001748006.1 | 41% | (many diffs) | |
| 19 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_001748005.1 | 34.1% | (many diffs) | |
| 20 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XM_017018421.1 | 33.5% | 31.4% | 1_303del;858_859ins44;909_910ins853 |
| 21 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_001748008.2 | 31.3% | (many diffs) | |
| 22 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_001748009.1 | 27.4% | (many diffs) | |
| 23 | human | 84680 | ACCS | 1-aminocyclopropane-1-carbo... | XR_001748010.1 | 26.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt cacccttcct caaaaggact tcagggctcc caccacctgt ctgggcccca 121 cctgcatgca ggacctgggc agtagccatg gggaagatct ggaaggagaa tgctccagaa 181 aactggacca gaagctgcca gagctccgtg gagtgggtga tcctgccatg atctcctctg 241 atacctccta cctgtcctct agaggaagaa tgattaaatg gttctgggat tcagctgagg 301 agggctacag gacctaccac atggatgagt atgatgagga caagaacccc agtggcatca 361 ttaacttggg caccagtgag aacaaactct gctttgacct gctgtcctgg cggctgagtc 421 agcgcgacat gcagagggtg gagccatccc tgctgcagta tgctgactgg aggggacatc 481 tgttcctccg ggaggaagtg gccaagttcc tgtctttcta ctgcaagagc ccagtacccc 541 tcagaccaga gaatgtggtt gtcctgaatg gtggtgcctc gctcttctct gctctggcca 601 cggtgctgtg tgaggccggg gaggctttcc tgatccccac cccttactat ggcgctatca 661 cacagcacgt gtgtctctat ggcaacatcc ggctggccta tgtctacctg gacagtgagg 721 tcactgggct agacacacgc cccttccagc tcacagtgga gaagctggag atggccctga 781 gagaagctca ctctgagggt gtgaaggtca aaggcctcat cctcatcagc ccccagaacc 841 ctctgggtga tgtatactcc cctgaagagc tacaggagta cctggtattt gccaagaggc 901 acaggctgca tgtgattgtg gatgaggtct acatgttgtc cgtgtttgag aagtctgttg 961 ggtaccgcag tgtcctaagc ctggaaaggc tccctgaccc ccagaggacc catgtgatgt 1021 gggcaaccag caaggacttc gggatgtctg ggctccgctt tggcacgctg tacacagaaa 1081 accaggatgt ggccactgcc gtggcttccc tctgccgcta ccacggcctc agtggcttgg 1141 tccagtacca gatggcacag ctgctccggg accgtgactg gatcaaccag gtgtacctgc 1201 cggaaaacca tgcccggctc aaggctgccc acacctatgt ctcagaagag cttagggcat 1261 tggggatccc cttcttgagt cgtggggctg gcttcttcat ctgggttgac ttgagaaagt 1321 acctgctcaa gggcaccttt gaggaggaaa tgctgctctg gcgccgcttt ttggacaaca 1381 aggtgctgct gtcctttggc aaggccttcg agtgtaaaga gcctggttgg tttcgctttg 1441 tcttctcaga ccaggtccac cggctttgcc tggggatgca gagggtccag caggtgcttg 1501 caggcaaatc ccaagtggca gaagaccccc gtccctctca gagccaggag ccaagtgacc 1561 aacgcaggtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1621 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1681 tcaacctctg gatta