Construct: ORF ccsbBroad304_09224
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010956.1_s304c1, BRDN0000401202
- Derived from:
- ccsbBroadEn_09224
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFSD9 (84804)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09224
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84804 | MFSD9 | major facilitator superfami... | NM_032718.5 | 99.9% | 100% | 1335C>T |
| 2 | human | 84804 | MFSD9 | major facilitator superfami... | NM_001322080.1 | 87% | 87.1% | 0_1ins183;1152C>T |
| 3 | human | 84804 | MFSD9 | major facilitator superfami... | NM_001322081.1 | 87% | 87.1% | 0_1ins183;1152C>T |
| 4 | human | 84804 | MFSD9 | major facilitator superfami... | XM_011512005.2 | 87% | 87.1% | 0_1ins183;1152C>T |
| 5 | human | 84804 | MFSD9 | major facilitator superfami... | XM_011512006.2 | 72.6% | 69.2% | (many diffs) |
| 6 | human | 84804 | MFSD9 | major facilitator superfami... | XM_011512007.2 | 70.1% | 67.9% | 0_1ins262;34_35ins161;912C>T |
| 7 | human | 84804 | MFSD9 | major facilitator superfami... | NR_136174.2 | 44.1% | (many diffs) | |
| 8 | human | 84804 | MFSD9 | major facilitator superfami... | XR_923047.2 | 36.7% | 1_32del;1367C>T;1455_3862del | |
| 9 | human | 84804 | MFSD9 | major facilitator superfami... | NR_136173.1 | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1488
- ORF length:
- 1422
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggtatgga gcttgggggt cactgggaca tgaactcggc cccgaggctg gtctcggaga 121 ccgcagagcg gaaacaggag cagaagacag gaaccgaggc ggaggctgcc gactccggtg 181 ccgtcggagc ccgccgcttc ctgctctgtc tctacttggt gggcttcttg gatttgtttg 241 gtgtcagcat ggttgtgcct ttattgagcc ttcatgtcaa gtcccttgga gcaagtccaa 301 cagttgctgg aatagtaggc tcctcctatg gcattttgca actcttttct agcacattgg 361 tgggctgctg gagcgatgta gtgggaagac ggtcttcctt gctggcatgc attctactca 421 gtgctctggg ctatctcctt ctcggagcag ccaccaatgt gtttctgttt gtcctggcta 481 gagtcccggc aggtattttt aaacacactc tctccatctc aagggctcta ctttctgatg 541 tggttccaga gaaggaacgg ccgcttgtaa tcggacactt caacacagcc tccggtgtgg 601 gcttcatctt gggccccgtg gtcggtggct atctcactga attagaggat gggttttatc 661 tcacagcctt catctgcttt ttggtcttca ttctcaatgc tggtctcgtt tggttctttc 721 catggaggga agcaaaaccg ggcagtacag agaagggcct gccattgcga aagacccatg 781 tgctgttggg aaggagccat gacacagtgc aggaggcagc caccagccgc agagccaggg 841 ccagcaagaa gactgcccag ccctgggtcg aagtagtgtt ggccttgcgg aacatgaaga 901 acctgctgtt ttccgaaatg tgggacatat ttctggtgcg cttgctgatg gccatggcag 961 tcatgctgta ctacagtaac tttgtcctgg ccctggagga gcgctttggg gtgcggccca 1021 aggtgacagg ctacctcatc agttacagca gcatgctggg ggccgtggcc ggccttgccc 1081 tggggccaat cctacggctg tacaagcaca actcgcaggc actgctgctg cattccagca 1141 tactcacctg cacactgctg ctgctctact ccttggcccc caccatgggt gcagttgtcc 1201 tctcctccac tctcctgtcc ttctccactg ccattggcag gacgtgcatc acggacctcc 1261 agctgactgt gggcggggcc caggccagcg gcacccttat tggcgtgggg cagtctgtga 1321 ctgcagtggg ccgcatcatc gcccctctcc tctcgggggt tgcccaggag gtcagccctt 1381 gcggcccccc cagcctgggt gctgtgttag ccttagtggc cattttcata atgtctctaa 1441 acaagcgaca ctctagtggt gatgggaata gtaaattaaa aagtgagtac ccaactttct 1501 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1561 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta