Construct: ORF ccsbBroad304_09421

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007944.1_s304c1, BRDN0000399228
Derived from:
ccsbBroadEn_09421
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DIS3L (115752)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09421
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323936.2 99.9% 100% 972T>C
2 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323945.2 99.9% 100% 972T>C
3 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_133375.5 99.9% 100% 972T>C
4 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323937.2 95.5% 95.5% 42_43ins129;843T>C
5 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323938.2 94.7% 94.7% 0_1ins153;819T>C
6 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323939.2 94.7% 94.7% 0_1ins153;819T>C
7 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323941.2 94.7% 94.7% 0_1ins153;819T>C
8 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323946.2 94.7% 94.7% 0_1ins153;819T>C
9 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323948.2 94% 89.4% (many diffs)
10 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323944.2 93.6% 93.6% 1_198del;1170T>C
11 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001143688.3 92% 92.1% 1_249del;1221T>C
12 human 115752 DIS3L DIS3 like exosome 3'-5' exo... XM_005254146.4 72.3% 72.2% (many diffs)
13 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323940.2 70.4% 69.3% 0_1ins691;51_52ins170;111T>C
14 human 115752 DIS3L DIS3 like exosome 3'-5' exo... NM_001323943.2 70.4% 69.3% 0_1ins691;51_52ins170;111T>C
15 mouse 213550 Dis3l DIS3 like exosome 3'-5' exo... NM_001177784.1 86.3% 91.7% (many diffs)
16 mouse 213550 Dis3l DIS3 like exosome 3'-5' exo... NM_172519.3 86.3% 91.7% (many diffs)
17 mouse 213550 Dis3l DIS3 like exosome 3'-5' exo... XM_006510982.3 86.3% 91.7% (many diffs)
18 mouse 213550 Dis3l DIS3 like exosome 3'-5' exo... XM_006510983.3 81.9% 86.9% (many diffs)
19 mouse 213550 Dis3l DIS3 like exosome 3'-5' exo... XM_011242700.1 81.9% 86.9% (many diffs)
20 mouse 213550 Dis3l DIS3 like exosome 3'-5' exo... NM_001001295.2 79.5% 84.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2979
ORF length:
2913
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca gacagcttgt caagctgtgc agcatcaaag aggcaggaga cagtataaca
121aactgcgaaa cctgctgaag gatgcgcgtc atgattgcat tctctttgct aatgaattcc
181agcaatgctg ctatctgcca cgggaaagag gagagtccat ggagaagtgg cagaccagga
241gcatatacaa cgcagctgtt tggtactatc atcactgcca ggacaggatg ccaattgtta
301tggtgacaga agatgaagag gcaattcagc agtatggaag tgaaacagaa ggagtattcg
361tgattacttt caagaattac ctggacaatt tctggcctga tttaaaagct gcccacgagc
421tttgtgattc tatccttcag tctcgacggg agagagagaa tgagagtcag gagagccatg
481ggaaggagta cccagaacat cttcccctgg aagtgttaga agctgggatt aaatctggac
541gctatatcca gggaattctg aatgtcaaca aacacagagc ccaaatagaa gcttttgttc
601gacttcaagg agccagcagt aaagattcag atttagtcag tgacatccta atccacggga
661tgaaggctcg aaaccgctca attcatggag atgtggtagt tgtggagctg cttcctaaaa
721atgaatggaa aggaagaacc gtagccctgt gtgagaatga ctgtgacgac aaggcttcgg
781gcgagtcccc aagtgagccc atgcctacag gtcgagtggt gggcatactt cagaagaact
841ggcgggatta tgtggtgaca tttccgtcca aagaagaggt ccaatctcag ggcaaaaatg
901ctcagaaaat cctggttaca ccttgggatt acagaattcc caaaattcga attagcactc
961agcaagcaga aaccctccag gacttcaggg tggtcgtgcg catcgattcc tgggagtcaa
1021catctgtgta tccaaacgga cattttgtgc gtgttttagg aagaatcgga gatctggaag
1081gggaaattgc aaccatcctg gtggaaaaca gtatttcagt tattcctttc tcagaagctc
1141agatgtgtga gatgccagta aacacaccag aaagtccctg gaaggtgagt cctgaagagg
1201aacaaaaacg taaagacttg aggaaaagcc atctcgtatt cagcattgac cccaaaggtt
1261gtgaagatgt ggatgacaca ctctcagtca gaaccttaaa taatggcaac ctggaacttg
1321gggtccacat cgcagatgta acacactttg tggcaccaaa ttcttacatt gatattgaag
1381ctagaacaag ggccaccact tattatctag cagatcgtcg ctatgacatg ctgccttccg
1441tcctcagtgc agatttgtgt tcccttctgg gaggcgttga taggtatgct gtaagcatca
1501tgtgggaact ggataaagcc tcttatgaaa ttaagaaagt gtggtatggc agaaccatta
1561ttcgatcagc atacaaactg ttctatgaag cagcccaaga actactggat ggaaacttaa
1621gcgttgttga tgatattcca gaattcaaag acttggatga gaagagcaga caagccaagc
1681tggaggagtt ggtgtgggca attggaaagc tgaccgacat agctcgccat gtcagagcta
1741aacgagacgg atgtggtgcc ctggaactgg aaggggtaga ggtttgcgta cagctagatg
1801acaaaaagaa cattcacgac ctcatcccca agcagcccct ggaagtccac gagacagtgg
1861ctgaatgcat gatcctggcc aaccactggg tcgccaaaaa gatctgggag agcttccctc
1921atcaggcctt gctgcgccag caccctcctc cacaccagga gttcttttca gaactccggg
1981aatgtgctaa agccaaaggc ttcttcatag atacacggtc caataaaaca ctggctgatt
2041ctctggataa tgcgaacgac ccccacgatc ccattgtgaa caggctactg cgctccatgg
2101ccacgcaggc catgtcgaat gctctgtact tctccaccgg atcctgtgcg gaggaggagt
2161tccatcatta cggtcttgca ttagataaat atacccactt tacttctcca ataagaagat
2221attcagatat tgtagtacac cgcttgttaa tggcagccat ttcaaaagat aagaaaatgg
2281aaattaaggg aaatctgttc agcaacaaag atcttgagga attatgcaga catatcaaca
2341acagaaacca agcagcacag cattctcaga agcagtctac tgagctcttc cagtgcatgt
2401acttcaaaga caaagaccct gccaccgagg agcgttgcat atctgacgga gttatttatt
2461caattagaac aaatggtgtg cttctattta taccaaggtt tgggattaaa ggtgctgctt
2521atctaaaaaa taaagatggt ttagtcatct catgtggccc agatagctgt tctgaatgga
2581aaccaggatc ccttcaacga tttcaaaaca aaattacctc tactacaaca gatggggaat
2641ctgttacgtt ccatttgttt gaccatgtaa ccgtaagaat atccatacag gcctcacgtt
2701gccattctga tacaatcaga cttgaaataa ttagtaacaa accatacaag ataccaaata
2761cagaacttat tcatcagagt tcccccttgc tgaagagtga gttagtgaaa gaagtaacta
2821aatctgtgga agaagctcag cttgcccaag aagtcaaagt aaacatcatt caggaggaat
2881atcaagaata tcgccaaaca aagggaagga gcctatacac acttctagag gagatacggg
2941acctagctct cctggatgtt tcaaacaatt atggaatatg cccaactttc ttgtacaaag
3001tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
3061gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)