Construct: ORF ccsbBroad304_09421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007944.1_s304c1, BRDN0000399228
- Derived from:
- ccsbBroadEn_09421
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIS3L (115752)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09421
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323936.2 | 99.9% | 100% | 972T>C |
| 2 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323945.2 | 99.9% | 100% | 972T>C |
| 3 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_133375.5 | 99.9% | 100% | 972T>C |
| 4 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323937.2 | 95.5% | 95.5% | 42_43ins129;843T>C |
| 5 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323938.2 | 94.7% | 94.7% | 0_1ins153;819T>C |
| 6 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323939.2 | 94.7% | 94.7% | 0_1ins153;819T>C |
| 7 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323941.2 | 94.7% | 94.7% | 0_1ins153;819T>C |
| 8 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323946.2 | 94.7% | 94.7% | 0_1ins153;819T>C |
| 9 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323948.2 | 94% | 89.4% | (many diffs) |
| 10 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323944.2 | 93.6% | 93.6% | 1_198del;1170T>C |
| 11 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001143688.3 | 92% | 92.1% | 1_249del;1221T>C |
| 12 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | XM_005254146.4 | 72.3% | 72.2% | (many diffs) |
| 13 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323940.2 | 70.4% | 69.3% | 0_1ins691;51_52ins170;111T>C |
| 14 | human | 115752 | DIS3L | DIS3 like exosome 3'-5' exo... | NM_001323943.2 | 70.4% | 69.3% | 0_1ins691;51_52ins170;111T>C |
| 15 | mouse | 213550 | Dis3l | DIS3 like exosome 3'-5' exo... | NM_001177784.1 | 86.3% | 91.7% | (many diffs) |
| 16 | mouse | 213550 | Dis3l | DIS3 like exosome 3'-5' exo... | NM_172519.3 | 86.3% | 91.7% | (many diffs) |
| 17 | mouse | 213550 | Dis3l | DIS3 like exosome 3'-5' exo... | XM_006510982.3 | 86.3% | 91.7% | (many diffs) |
| 18 | mouse | 213550 | Dis3l | DIS3 like exosome 3'-5' exo... | XM_006510983.3 | 81.9% | 86.9% | (many diffs) |
| 19 | mouse | 213550 | Dis3l | DIS3 like exosome 3'-5' exo... | XM_011242700.1 | 81.9% | 86.9% | (many diffs) |
| 20 | mouse | 213550 | Dis3l | DIS3 like exosome 3'-5' exo... | NM_001001295.2 | 79.5% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2979
- ORF length:
- 2913
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gacagcttgt caagctgtgc agcatcaaag aggcaggaga cagtataaca 121 aactgcgaaa cctgctgaag gatgcgcgtc atgattgcat tctctttgct aatgaattcc 181 agcaatgctg ctatctgcca cgggaaagag gagagtccat ggagaagtgg cagaccagga 241 gcatatacaa cgcagctgtt tggtactatc atcactgcca ggacaggatg ccaattgtta 301 tggtgacaga agatgaagag gcaattcagc agtatggaag tgaaacagaa ggagtattcg 361 tgattacttt caagaattac ctggacaatt tctggcctga tttaaaagct gcccacgagc 421 tttgtgattc tatccttcag tctcgacggg agagagagaa tgagagtcag gagagccatg 481 ggaaggagta cccagaacat cttcccctgg aagtgttaga agctgggatt aaatctggac 541 gctatatcca gggaattctg aatgtcaaca aacacagagc ccaaatagaa gcttttgttc 601 gacttcaagg agccagcagt aaagattcag atttagtcag tgacatccta atccacggga 661 tgaaggctcg aaaccgctca attcatggag atgtggtagt tgtggagctg cttcctaaaa 721 atgaatggaa aggaagaacc gtagccctgt gtgagaatga ctgtgacgac aaggcttcgg 781 gcgagtcccc aagtgagccc atgcctacag gtcgagtggt gggcatactt cagaagaact 841 ggcgggatta tgtggtgaca tttccgtcca aagaagaggt ccaatctcag ggcaaaaatg 901 ctcagaaaat cctggttaca ccttgggatt acagaattcc caaaattcga attagcactc 961 agcaagcaga aaccctccag gacttcaggg tggtcgtgcg catcgattcc tgggagtcaa 1021 catctgtgta tccaaacgga cattttgtgc gtgttttagg aagaatcgga gatctggaag 1081 gggaaattgc aaccatcctg gtggaaaaca gtatttcagt tattcctttc tcagaagctc 1141 agatgtgtga gatgccagta aacacaccag aaagtccctg gaaggtgagt cctgaagagg 1201 aacaaaaacg taaagacttg aggaaaagcc atctcgtatt cagcattgac cccaaaggtt 1261 gtgaagatgt ggatgacaca ctctcagtca gaaccttaaa taatggcaac ctggaacttg 1321 gggtccacat cgcagatgta acacactttg tggcaccaaa ttcttacatt gatattgaag 1381 ctagaacaag ggccaccact tattatctag cagatcgtcg ctatgacatg ctgccttccg 1441 tcctcagtgc agatttgtgt tcccttctgg gaggcgttga taggtatgct gtaagcatca 1501 tgtgggaact ggataaagcc tcttatgaaa ttaagaaagt gtggtatggc agaaccatta 1561 ttcgatcagc atacaaactg ttctatgaag cagcccaaga actactggat ggaaacttaa 1621 gcgttgttga tgatattcca gaattcaaag acttggatga gaagagcaga caagccaagc 1681 tggaggagtt ggtgtgggca attggaaagc tgaccgacat agctcgccat gtcagagcta 1741 aacgagacgg atgtggtgcc ctggaactgg aaggggtaga ggtttgcgta cagctagatg 1801 acaaaaagaa cattcacgac ctcatcccca agcagcccct ggaagtccac gagacagtgg 1861 ctgaatgcat gatcctggcc aaccactggg tcgccaaaaa gatctgggag agcttccctc 1921 atcaggcctt gctgcgccag caccctcctc cacaccagga gttcttttca gaactccggg 1981 aatgtgctaa agccaaaggc ttcttcatag atacacggtc caataaaaca ctggctgatt 2041 ctctggataa tgcgaacgac ccccacgatc ccattgtgaa caggctactg cgctccatgg 2101 ccacgcaggc catgtcgaat gctctgtact tctccaccgg atcctgtgcg gaggaggagt 2161 tccatcatta cggtcttgca ttagataaat atacccactt tacttctcca ataagaagat 2221 attcagatat tgtagtacac cgcttgttaa tggcagccat ttcaaaagat aagaaaatgg 2281 aaattaaggg aaatctgttc agcaacaaag atcttgagga attatgcaga catatcaaca 2341 acagaaacca agcagcacag cattctcaga agcagtctac tgagctcttc cagtgcatgt 2401 acttcaaaga caaagaccct gccaccgagg agcgttgcat atctgacgga gttatttatt 2461 caattagaac aaatggtgtg cttctattta taccaaggtt tgggattaaa ggtgctgctt 2521 atctaaaaaa taaagatggt ttagtcatct catgtggccc agatagctgt tctgaatgga 2581 aaccaggatc ccttcaacga tttcaaaaca aaattacctc tactacaaca gatggggaat 2641 ctgttacgtt ccatttgttt gaccatgtaa ccgtaagaat atccatacag gcctcacgtt 2701 gccattctga tacaatcaga cttgaaataa ttagtaacaa accatacaag ataccaaata 2761 cagaacttat tcatcagagt tcccccttgc tgaagagtga gttagtgaaa gaagtaacta 2821 aatctgtgga agaagctcag cttgcccaag aagtcaaagt aaacatcatt caggaggaat 2881 atcaagaata tcgccaaaca aagggaagga gcctatacac acttctagag gagatacggg 2941 acctagctct cctggatgtt tcaaacaatt atggaatatg cccaactttc ttgtacaaag 3001 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 3061 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta