Construct: ORF ccsbBroad304_09549

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010747.1_s304c1, BRDN0000404642
Derived from:
ccsbBroadEn_09549
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EGFLAM (133584)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09549
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 133584 EGFLAM EGF like, fibronectin type ... NM_152403.4 99.9% 99.9% 1418C>T;3000G>A
2 human 133584 EGFLAM EGF like, fibronectin type ... NM_001205301.2 99.1% 99% 1418C>T;2465_2488del;3024G>A
3 human 133584 EGFLAM EGF like, fibronectin type ... NM_182798.3 76.6% 76.3% (many diffs)
4 human 133584 EGFLAM EGF like, fibronectin type ... NM_182801.3 15% 15% 0_1ins2571;429G>A
5 mouse 268780 Egflam EGF-like, fibronectin type ... NM_178748.6 84.8% 88.3% (many diffs)
6 mouse 268780 Egflam EGF-like, fibronectin type ... XM_017316628.1 84.8% 88.3% (many diffs)
7 mouse 268780 Egflam EGF-like, fibronectin type ... NM_001289496.1 84.1% 87.5% (many diffs)
8 mouse 268780 Egflam EGF-like, fibronectin type ... NM_001289498.1 72% 73% (many diffs)
9 mouse 268780 Egflam EGF-like, fibronectin type ... XM_017316629.1 70.4% 69.5% (many diffs)
10 mouse 268780 Egflam EGF-like, fibronectin type ... XM_006519991.1 65.8% 69.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3093
ORF length:
3027
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tttaatccga ggcgtcttgc tccggctcct gctcctggct tccagcctcg
121gacccggcgc ggtgtcgctc cgagcggcca tccgaaaacc aggcaaggta gggcctcctc
181ttgacatcaa gctgggcgca ttgaactgta cggctttcag catccagtgg aaaatgccaa
241ggcatcctgg aagtcccatc cttgggtaca ctgtctttta ctctgaggtt ggcgcagata
301aatccctgca ggagcagttg cacagcgtgc ctctcagccg ggacatcccg accacggagg
361aagtgattgg agatttgaaa ccaggcactg aatatcgtgt gagcatagca gcttacagcc
421aggctggcaa agggcggctg agctctcctc ggcatgtcac cactttgtcc caagattcct
481gcctgcctcc tgcagctccc cagcagccac atgtcattgt ggtttcggat tctgaggtgg
541ccctgtcttg gaaacctgga gcgagtgaag gaagcgcccc tattcagtac tattctgtgg
601aattcatcag gccagatttc gacaagaagt ggacctcaat ccatgagcgg atccagatgg
661actccatggt tatcaagggc ctcgatccag ataccaacta ccagtttgcc gtgagggcaa
721tgaattccca tggccccagc ccccgcagct ggcccagtga catcatccgg accctctgcc
781ctgaggaggc gggaagtggc cgctatggac cccgttatat caccgacatg ggagctggtg
841aggatgatga aggatttgaa gacgacttag atttggatat ttcctttgag gaggttaaac
901cacttcctgc taccaaagga gggaataaga aatttttggt ggaaagcaag aagatgtcta
961tatctaaccc aaagaccatt tctaggctca tcccccctac ctcagcatct ctccctgtga
1021ccacggtggc tccccagccc attcccatac agagaaaggg gaagaatggt gtggccataa
1081tgtcaaggct ctttgacatg ccttgtgatg aaactctctg ctctgctgac agcttctgtg
1141tcaatgacta cacctggggg ggctcgcgat gccagtgcac cctgggcaaa ggtggtgaga
1201gctgctcaga agatattgtt atccagtatc ctcagttctt tggccactcc tatgtaacgt
1261ttgaacctct gaagaattct tatcaggcat ttcaaattac tcttgaattt agggcggagg
1321cagaggatgg cttactgctc tactgtgggg agaacgaaca cgggaggggg gatttcatgt
1381ccctggctat catccgacgc tccctgcagt tcaggtttaa ttgtggaact ggggttgcca
1441tcatcgtaag tgagaccaaa atcaaactag ggggttggca catggttatg ctctacagag
1501atgggctgaa cgggctgctg cagctgaaca atggcacccc agtgacaggc cagtctcagg
1561gccaatacag taaaattact ttccggacac ctctctatct tggtggcgct cccagcgctt
1621actggttggt tagagcaaca gggacaaacc gaggctttca aggctgtgtg cagtcgctcg
1681ctgtgaatgg gaggagaatt gacatgaggc cctggcccct gggaaaagca ctcagtgggg
1741ctgatgtggg ggaatgcagc agtggaatct gtgatgaggc ctcgtgcatc catggtggca
1801cctgcacagc aatcaaagcc gactcctaca tttgcctctg tccccttggg tttaaaggtc
1861gacactgtga agatgctttc accttgacca ttcctcagtt cagagagtct ctgagatctt
1921acgctgcaac tccctggcca ctggagcccc agcattacct ttccttcatg gaatttgaga
1981tcacatttcg gccagactca ggagatggtg tcctcctgta cagctatgac acaggcagca
2041aagacttcct gtccatcaac ttggcagggg gccacgtgga gttccgcttt gactgtggct
2101ctgggaccgg tgtcctcagg agtgaagatc ccctcaccct gggcaactgg cacgagcttc
2161gtgtatctcg cacagcaaag aatggaatct tacaggtgga taagcagaag atagtggagg
2221gaatggcaga gggaggcttc acacagatta agtgcaacac agacattttc attggcggag
2281tccccaatta tgatgatgtg aagaagaact cgggtgtcct gaagcctttc agcgggagca
2341tccagaagat catcctgaat gaccgaacca tccatgtgaa gcatgacttc acctccggag
2401tgaatgtgga gaatgcggcc cacccctgtg tgagagcccc ttgtgcccat gggggcagct
2461gccggcccag gaaggagggc tatgactgtg actgcccctt gggctttgag gggcttcact
2521gccagaaagc gatcatagaa gccattgaga tcccgcagtt tatcggccgc agttacctga
2581cgtatgacaa cccagatatc ttgaagaggg tgtcaggatc aagatcaaat gtgttcatga
2641ggtttaaaac aactgccaag gatggccttt tgctgtggag gggagacagc cccatgagac
2701ccaacagcga cttcatttcc ttgggccttc gggatggagc cctcgtgttc agctataacc
2761tgggcagtgg tgtggcatcc atcatggtga atggctcctt caacgatggt cggtggcacc
2821gagttaaggc cgttagggat ggccagtcag gaaagataac cgtggatgac tatggagcca
2881gaacaggcaa atccccaggc atgatgcggc agcttaacat caatggagct ctgtatgtgg
2941gtggaatgaa ggaaattgct ctgcacacta acaggcaata tatgagaggg ctcgtgggct
3001gtatctctca cttcaccctg tccaccgatt accacatttc cctcgtggaa gatgccgtgg
3061atggaaaaaa catcaacact tgtggagcca agtacccaac tttcttgtac aaagtggttg
3121gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
3181accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)