Construct: ORF ccsbBroad304_09658
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018519.1_s304c1, BRDN0000461526
- Derived from:
- ccsbBroadEn_09658
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFSD4A (148808)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09658
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148808 | MFSD4A | major facilitator superfami... | NM_181644.5 | 99.6% | 99.6% | (many diffs) |
| 2 | human | 148808 | MFSD4A | major facilitator superfami... | XM_011509229.3 | 82.8% | 82.8% | (many diffs) |
| 3 | mouse | 213006 | Mfsd4a | major facilitator superfami... | NM_001114662.1 | 86.9% | 86.7% | (many diffs) |
| 4 | mouse | 213006 | Mfsd4a | major facilitator superfami... | NM_172510.4 | 83.6% | 84% | (many diffs) |
| 5 | mouse | 213006 | Mfsd4a | major facilitator superfami... | XM_006529344.3 | 83.6% | 84% | (many diffs) |
| 6 | mouse | 213006 | Mfsd4a | major facilitator superfami... | XR_001783717.1 | 51.2% | (many diffs) | |
| 7 | mouse | 213006 | Mfsd4a | major facilitator superfami... | XM_006529345.3 | 46.2% | 46.4% | (many diffs) |
| 8 | mouse | 213006 | Mfsd4a | major facilitator superfami... | XM_017319650.1 | 46.2% | 46.4% | (many diffs) |
| 9 | mouse | 213006 | Mfsd4a | major facilitator superfami... | XR_001783716.1 | 32.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1608
- ORF length:
- 1542
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcgacggc cgcgtttcgg ggctgctccg ccgcaacctg cagcccacgc 121 tcacctactg gagcgtcttc ttcagcttcg gcctgtgcat cgccttcctg gggcccacgc 181 tgctggacct gcgctgtcag acgcacagct cgctgcccca gatctcctgg gtcttcttct 241 cgcagcagct ctgcctcctg ctgggcagcg ccctcggggg cgtcttcaaa aggaccctgg 301 cccagtcact atgggccctg ttcacctcct ctctggccat ctccctggtg tttgccgtca 361 tccccttctg ccgcgacgtg aaggtgctgg cctcagtcat ggcgctggcg ggcttggcca 421 tgggctgcat cgacaccgtg gccaacatgc agctggtaag gatgtaccag aaggactcgg 481 ccgtcttcct ccaggtgctc catttcttcg tgggctttgg tgctctgctg agccccctta 541 ttgctgaccc tttcctgtct gaggccaact gcttgcctgc caatagcacg gccaacacca 601 cctcccgagg ccacctgttc catgtctcca gggtgctggg ccagcaccac gtagatgcca 661 agccttggtc caaccagacg ttcccagggc tgactccaaa ggacggggca gggacccgag 721 tgtcctatgc cttctggatc atggccatca tcaatcttcc agtgcccatg gctgtgctga 781 tgctgctgtc caaggagcgg ctgctgacct gctgtcccca gaggaggccc ctgcttctgt 841 ctgctgatga gcttgccttg gagacacagc ctcctgagaa ggaagatgcc tcctcactgc 901 ccccaaagtt tcagtcacac ctagggcatg aggacctgtt cagctgctgc caaaggaaga 961 acctcagagg agccccttat tccttctttg ccatccacat cacggccgcc ctggtcctgt 1021 tcatgacgga tgggttgacg ggtgcctatt ccgccttcgt gtacagctat gctgtggaga 1081 agcccctgtc tgtgggacac aaggtggctg gctacctccc cagcctcttc tggggcttca 1141 tcacactggg ccggctcctc tccattccca tatcctcaag aatgaagccg gccaccatgg 1201 ttttcatcaa cgtggttggc gtggtggtga cgttcctggt gctgcttatt ttctcctaca 1261 acgtcgtctt cctgttcgtg gggacggcaa gcctgggcct gtttctcagc agcacctttc 1321 ccagcatgct ggcctacacg gaggactcgc tgcagtacaa aggctgtgca accacagtgc 1381 tggtgacagg ggcaggagtt ggcgagatgg tgctgcagat gctggttggt tcgatattcc 1441 aggctcaggg cagctatagt ttcctggtct gtggcgtgat ctttggttgt ctggctttta 1501 ccttctatat cttgctcctg tttttccaca ggatgcaccc tggactccca tcagttccta 1561 cccaagacag atcaattgga atggaaaact ctgagtgcta ccagaggtac ccaactttct 1621 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1681 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta