Transcript: Mouse NM_001114662.1

Mus musculus major facilitator superfamily domain containing 4A (Mfsd4a), transcript variant b, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Mfsd4a (213006)
Length:
3842
CDS:
171..1703

Additional Resources:

NCBI RefSeq record:
NM_001114662.1
NBCI Gene record:
Mfsd4a (213006)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001114662.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329070 GAGGACATCCTCCAGTATAAA pLKO_005 1434 CDS 100% 15.000 12.000 N Mfsd4a n/a
2 TRCN0000329010 TGCCATGACGTGAAGGTATTG pLKO_005 474 CDS 100% 10.800 8.640 N Mfsd4a n/a
3 TRCN0000190351 CGTATCCTATGCCTTCTGGAT pLKO.1 815 CDS 100% 2.640 2.112 N Mfsd4a n/a
4 TRCN0000329011 ACGGGATGATGGGCGCTTATT pLKO_005 1123 CDS 100% 13.200 9.240 N Mfsd4a n/a
5 TRCN0000329012 ATGCAGTTGGTGAGGATATAC pLKO_005 552 CDS 100% 13.200 9.240 N Mfsd4a n/a
6 TRCN0000200735 GCCTGACACTTTAGTTATCTT pLKO.1 1831 3UTR 100% 5.625 3.938 N Mfsd4a n/a
7 TRCN0000190440 CTGGATCATGGCTCTCATCAA pLKO.1 830 CDS 100% 4.950 3.465 N Mfsd4a n/a
8 TRCN0000201052 CCCTCTTCTATACAGAAGGTA pLKO.1 3009 3UTR 100% 0.300 0.210 N Mfsd4a n/a
9 TRCN0000190964 CCTCTCAAGTGCTGGGATTAA pLKO.1 2422 3UTR 100% 13.200 6.600 Y Nop16 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001114662.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09658 pDONR223 100% 86.9% 86.7% None (many diffs) n/a
2 ccsbBroad304_09658 pLX_304 0% 86.9% 86.7% V5 (many diffs) n/a
3 TRCN0000478122 TCCTTATCATACGCTCACACGTCC pLX_317 1.7% 86.9% 86.7% V5 (many diffs) n/a
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