Construct: ORF ccsbBroad304_09693

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008234.1_s304c1, BRDN0000411813
Derived from:
ccsbBroadEn_09693
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC38A9 (153129)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09693
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 153129 SLC38A9 solute carrier family 38 me... NM_001349382.1 99.8% 99.8% 531G>A;545G>C;609T>C
2 human 153129 SLC38A9 solute carrier family 38 me... NM_001349383.1 99.8% 99.8% 531G>A;545G>C;609T>C
3 human 153129 SLC38A9 solute carrier family 38 me... NM_001349384.1 99.8% 99.8% 531G>A;545G>C;609T>C
4 human 153129 SLC38A9 solute carrier family 38 me... NM_173514.4 99.8% 99.8% 531G>A;545G>C;609T>C
5 human 153129 SLC38A9 solute carrier family 38 me... XM_006714539.3 99.8% 99.8% 531G>A;545G>C;609T>C
6 human 153129 SLC38A9 solute carrier family 38 me... XM_011543174.1 99.8% 99.8% 531G>A;545G>C;609T>C
7 human 153129 SLC38A9 solute carrier family 38 me... XM_011543176.1 94.4% 94.4% (many diffs)
8 human 153129 SLC38A9 solute carrier family 38 me... XM_017009077.1 94.4% 94.4% (many diffs)
9 human 153129 SLC38A9 solute carrier family 38 me... XM_011543177.1 93.4% 93.4% (many diffs)
10 human 153129 SLC38A9 solute carrier family 38 me... XM_024454369.1 93.4% 93.4% (many diffs)
11 human 153129 SLC38A9 solute carrier family 38 me... XM_011543178.1 89.8% 89.6% 526_527ins171
12 human 153129 SLC38A9 solute carrier family 38 me... XM_017009078.2 89.8% 89.6% 526_527ins171
13 human 153129 SLC38A9 solute carrier family 38 me... NM_001258286.1 88.5% 88.5% (many diffs)
14 human 153129 SLC38A9 solute carrier family 38 me... NM_001349385.1 88.2% 88.2% (many diffs)
15 human 153129 SLC38A9 solute carrier family 38 me... XM_017009080.1 88.2% 88.2% (many diffs)
16 human 153129 SLC38A9 solute carrier family 38 me... NM_001258287.1 87.8% 87.1% (many diffs)
17 human 153129 SLC38A9 solute carrier family 38 me... XM_017009081.1 82.8% 82.8% (many diffs)
18 human 153129 SLC38A9 solute carrier family 38 me... XM_011543180.1 81.8% 81.8% (many diffs)
19 human 153129 SLC38A9 solute carrier family 38 me... XM_017009082.1 81.8% 81.8% (many diffs)
20 human 153129 SLC38A9 solute carrier family 38 me... XM_011543182.1 80.9% 76.8% (many diffs)
21 human 153129 SLC38A9 solute carrier family 38 me... XM_011543183.1 78.2% 78% 526_527ins171;585_586ins195
22 human 153129 SLC38A9 solute carrier family 38 me... NM_001282429.1 77.7% 77.7% (many diffs)
23 human 153129 SLC38A9 solute carrier family 38 me... XM_011543184.1 71.8% 71.6% 526_527ins171;585_586ins303
24 human 153129 SLC38A9 solute carrier family 38 me... NR_146165.1 64% (many diffs)
25 human 153129 SLC38A9 solute carrier family 38 me... XR_948237.2 61.8% (many diffs)
26 human 153129 SLC38A9 solute carrier family 38 me... XR_001742002.1 58.8% (many diffs)
27 human 153129 SLC38A9 solute carrier family 38 me... XR_948238.1 57.6% (many diffs)
28 human 153129 SLC38A9 solute carrier family 38 me... NR_047649.1 56% (many diffs)
29 mouse 268706 Slc38a9 solute carrier family 38, m... NM_178746.4 89% 91.4% (many diffs)
30 mouse 268706 Slc38a9 solute carrier family 38, m... XM_006517670.2 89% 91.4% (many diffs)
31 mouse 268706 Slc38a9 solute carrier family 38, m... XM_006517671.3 86.4% 87.7% (many diffs)
32 mouse 268706 Slc38a9 solute carrier family 38, m... XM_006517672.2 86.4% 87.7% (many diffs)
33 mouse 268706 Slc38a9 solute carrier family 38, m... XM_006517673.3 79.8% 82.7% (many diffs)
34 mouse 268706 Slc38a9 solute carrier family 38, m... XM_006517675.3 79.8% 82.7% (many diffs)
35 mouse 268706 Slc38a9 solute carrier family 38, m... XM_017315519.1 79.8% 82.7% (many diffs)
36 mouse 268706 Slc38a9 solute carrier family 38, m... XR_873763.2 22% (many diffs)
37 mouse 268706 Slc38a9 solute carrier family 38, m... XR_873764.2 17.2% (many diffs)
38 mouse 268706 Slc38a9 solute carrier family 38, m... XR_873762.2 17.1% (many diffs)
39 mouse 268706 Slc38a9 solute carrier family 38, m... XR_001780783.1 16.7% (many diffs)
40 mouse 268706 Slc38a9 solute carrier family 38, m... XR_382737.3 15.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1752
ORF length:
1683
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcaaatatg aatagtgatt ctaggcatct tggcacctct gaggtagatc
121atgaaagaga tcctggacct atgaatatcc agtttgagcc atcggatcta agatccaaaa
181ggcctttctg tatagagccc acaaacatcg tgaatgtgaa tcatgtcatt cagagggtta
241gtgaccatgc ctctgccatg aacaagagaa ttcattacta cagccggctc accactcctg
301cagacaaggc actgattgcc ccagaccatg tagttccagc tccagaagag tgctatgtgt
361atagtccatt gggctctgct tataaacttc aaagttacac tgaaggatac ggtaaaaaca
421ccagtttagt aaccattttt atgatttgga ataccatgat gggaacatct atactaagca
481ttccttgggg cataaaacag gctggattta ctactggaat gtgtgtcatc atactgatgg
541gccttttaac actttattgc tgctacagag tagtgaaatc acggactatg atgttttcat
601tggataccac tacctgggaa tatccagatg tctgcagaca ttatttcggc tcctttgggc
661agtggtcgag tctcctcttc tccttggtgt ctctcattgg agcaatgata gtttattggg
721tgcttatgtc aaattttctt tttaatactg gaaagtttat ttttaatttt attcatcaca
781ttaatgacac agacactata ctgagtacca ataatagcaa ccctgtgatt tgtccaagtg
841ccgggagtgg aggccatcct gacaacagct ctatgatttt ctatgccaat gacacaggag
901cccaacagtt tgaaaagtgg tgggataagt ccaggacagt ccccttttat cttgtagggc
961tcctcctccc actgctcaat ttcaagtctc cttcattttt ttcaaaattt aatatcctag
1021gcacagtgtc tgtcctttat ttgattttcc ttgtcacctt taaggctgtt cgcttgggat
1081ttcatttgga atttcattgg tttataccaa cagaattttt tgtaccagag ataagatttc
1141agtttccaca gctgactgga gtgcttaccc ttgctttttt tattcataat tgtatcatca
1201cactcttgaa gaacaacaag aaacaagaaa acaatgtgag ggacttgtgc attgcttata
1261tgctggtgac attaacttat ctctatattg gagtcctggt ttttgcttca tttccttcac
1321caccattatc caaagattgt attgagcaga attttttaga caacttccct agcagtgaca
1381ccctgtcctt cattgcaagg atattcctgc tgttccagat gatgactgta tacccactct
1441taggctacct ggctcgtgtc cagcttttgg gccatatctt cggtgacatt tatcctagca
1501ttttccatgt gctgattctt aatctaatta ttgtgggagc tggagtgatc atggcctgtt
1561tctacccaaa cataggaggg atcataagat attcaggagc agcatgtgga ctggcctttg
1621tattcatata cccatctctc atctatataa tttccctcca ccaagaagag cgtctgacat
1681ggcctaaatt aatcttccac gttttcatca tcattttggg cgtggctaac ctgattgttc
1741agttttttat gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1801tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1861caatcaacct ctggatta

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