Construct: ORF ccsbBroad304_09738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018550.1_s304c1, BRDN0000403824
- Derived from:
- ccsbBroadEn_09738
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FSIP1 (161835)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09738
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161835 | FSIP1 | fibrous sheath interacting ... | NM_001324338.2 | 99.8% | 99.6% | 1061G>A;1204T>C;1476C>T |
| 2 | human | 161835 | FSIP1 | fibrous sheath interacting ... | NM_152597.5 | 99.8% | 99.6% | 1061G>A;1204T>C;1476C>T |
| 3 | human | 161835 | FSIP1 | fibrous sheath interacting ... | XM_011521305.3 | 99.8% | 99.6% | 1061G>A;1204T>C;1476C>T |
| 4 | human | 161835 | FSIP1 | fibrous sheath interacting ... | XM_011521306.2 | 99.8% | 99.6% | 1061G>A;1204T>C;1476C>T |
| 5 | human | 161835 | FSIP1 | fibrous sheath interacting ... | XM_011521307.3 | 70.8% | 67.2% | (many diffs) |
| 6 | human | 161835 | FSIP1 | fibrous sheath interacting ... | XM_011521309.3 | 67.8% | 67.9% | (many diffs) |
| 7 | human | 161835 | FSIP1 | fibrous sheath interacting ... | XM_011521311.3 | 63.6% | 63.5% | (many diffs) |
| 8 | human | 161835 | FSIP1 | fibrous sheath interacting ... | XM_017021972.2 | 63.6% | 63.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1809
- ORF length:
- 1743
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tattataaag ggaaacctag atggaatttc aaaaccagct tcaaattcaa 121 gaatacgccc tgggagcaga agttcaaatg cttctttgga ggtgctctca acagaaccag 181 gatccttcaa ggtcgatact gcaagcaact tgaactctgg taaagaggac cactccgaaa 241 gcagtaatac agagaacaga agaactagta atgatgataa gcaggaaagc tgctctgaga 301 aaataaaatt ggctgaagag ggatcagatg aagatctgga tttggttcaa catcagataa 361 tctctgagtg ttcagatgaa cccaaattaa aagaattaga ttctcaactt caagatgcta 421 ttcagaagat gaaaaaactt gataaaatat tggcaaagaa acaacgcaga gaaaaagaaa 481 ttaagaagca aggtctagaa atgagaataa agctgtggga agaaattaag tctgcaaaat 541 atagtgaagc ttggcaaagt aaagaggaga tggaaaatac aaaaaaattt ttatctttga 601 ctgctgtttc tgaagaaact gttggtcctt ctcatgagga ggaagacacc ttttcctcag 661 tgtttcatac tcaaatccct ccagaagaat atgaaatgca gatgcagaaa ctcaataaag 721 attttacctg tgatgtggaa agaaatgagt cattgatcaa atcaggaaag aaacctttct 781 cgaatacaga aaagattgag ctcaggggta aacacaacca ggattttatt aagagaaaca 841 ttgagttggc caaggaatca agaaacccag tggttatggt tgacagagag aagaaaaggc 901 tggttgagct tttgaaggac ttggatgaga aagattccgg gctctccagt tctgagggtg 961 atcagtctgg ctgggtggtc ccagtaaaag gatatgaact tgcagtcacc cagcatcagc 1021 agcttgctga aattgatata aaactccaag aactctctgc agcctcccct acaatttcca 1081 gtttttctcc aagacttgaa aatcggaata atcagaaacc tgaccatgat ggtgaaagaa 1141 atatggaagt aactccagga gaaaagatac ttaggaacac caaagagcaa cgcgatctgc 1201 ataatcggct gagagagatt gatgaaaagc tgaaaatgat gaaggaaaat gtgttagagt 1261 ccacatcacg tctctctgaa gaacagttaa agtgtcttct ggatgaatgc atacttaaac 1321 aaaaatccat cattaaactt tcttcagaaa gaaaaaagga agacattgag gacgtaacac 1381 ctgtgttccc ccagctttcc aggtccatca tctctaaatt gctaaatgaa tcagaaacaa 1441 aggtccagaa aactgaggta gaagatgcag atatgcttga gagtgaagaa tgtgaagctt 1501 ctaaaggcta ctatctcact aaagccttga ctggacataa tatgtcagaa gctcttgtca 1561 ctgaagcaga gaatatgaaa tgccttcaat tttccaagga cgttattatt agtgacacaa 1621 aagactattt tatgtcgaag actcttggca ttgggagact gaaaaggccc tccttcttag 1681 atgatccact gtatggtatc agtgtgagcc tttcatcaga agaccaacat ctgaaactca 1741 gttctccaga gaatacaata gcagatgagc aggagactaa agatgcagca gaagaatgta 1801 aagaacccta ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 1861 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 1921 caacctctgg atta