Transcript: Human XM_011521305.3

PREDICTED: Homo sapiens fibrous sheath interacting protein 1 (FSIP1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FSIP1 (161835)
Length:
4980
CDS:
211..1956

Additional Resources:

NCBI RefSeq record:
XM_011521305.3
NBCI Gene record:
FSIP1 (161835)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011521305.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000172976 GCAAGCAACTTGAACTCTGGT pLKO.1 346 CDS 100% 2.640 2.112 N FSIP1 n/a
2 TRCN0000242495 CAGATGCAGAAACTCAATAAA pLKO_005 844 CDS 100% 15.000 10.500 N FSIP1 n/a
3 TRCN0000242498 CTCAGTGTTTCATACTCAAAT pLKO_005 801 CDS 100% 13.200 9.240 N FSIP1 n/a
4 TRCN0000242496 AGAATGTAAAGAACCCTAATC pLKO_005 1938 CDS 100% 10.800 7.560 N FSIP1 n/a
5 TRCN0000242499 CAGATGAGCAGGAGACTAAAG pLKO_005 1907 CDS 100% 10.800 7.560 N FSIP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011521305.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09738 pDONR223 100% 99.8% 99.6% None 1061G>A;1204T>C;1476C>T n/a
2 ccsbBroad304_09738 pLX_304 0% 99.8% 99.6% V5 (not translated due to frame shift) 1061G>A;1204T>C;1476C>T n/a
3 TRCN0000479026 GTATTGGCCTGAGGACTCTGATTA pLX_317 22.9% 99.8% 99.6% V5 (not translated due to frame shift) 1061G>A;1204T>C;1476C>T n/a
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