Construct: ORF ccsbBroad304_09953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004463.1_s304c1, BRDN0000401585
- Derived from:
- ccsbBroadEn_09953
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSDMA (284110)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09953
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284110 | GSDMA | gasdermin A | NM_178171.5 | 99.8% | 99.5% | 382G>T;941C>A |
| 2 | human | 284110 | GSDMA | gasdermin A | XM_006721832.3 | 99.8% | 99.5% | 382G>T;941C>A |
| 3 | human | 284110 | GSDMA | gasdermin A | XM_017024502.2 | 97.8% | 97.3% | 382G>T;703_704ins27;914C>A |
| 4 | human | 284110 | GSDMA | gasdermin A | XM_011524651.3 | 68% | 67.8% | 0_1ins426;515C>A |
| 5 | human | 284110 | GSDMA | gasdermin A | XM_017024503.1 | 56.3% | 55.9% | 382G>T;750_751insGGGGACGTACACGAAG;753_754ins566 |
| 6 | human | 284110 | GSDMA | gasdermin A | XM_017024504.2 | 54.3% | 53.7% | (many diffs) |
| 7 | mouse | 57911 | Gsdma | gasdermin A | NM_021347.4 | 85.5% | 88.3% | (many diffs) |
| 8 | mouse | 57911 | Gsdma | gasdermin A | XM_006533850.1 | 82.4% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1404
- ORF length:
- 1335
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccatgttt gaaaatgtca cccgggccct ggccagacag ctaaaccctc 121 gaggggacct gacaccactt gacagcctca tcgacttcaa gcgcttccat cccttctgcc 181 tggtgctgag gaagaggaag agcacgctct tctggggggc ccggtacgtc cgcaccgact 241 acacgctgct ggatgtgctt gagcccggca gctcaccttc agacccaaca gacactggga 301 attttggctt taagaatatg ctggacaccc gagtggaggg agatgtggat gtaccaaaga 361 cggtgaaggt gaagggaacg gcagggctct cgcagaacag cactctggag gtccagacac 421 tcagtgtggc tcccaaggcc ctggagacct tgcaggagag gaagctggca gcagaccacc 481 cattcctgaa ggagatgcaa gatcaagggg agaacctgta tgtggtgatg gaggtggtgg 541 agacggtgca ggaggtcaca ctggagcgag ccggcaaggc agaggcctgc ttctccctcc 601 ccttcttcgc cccattgggg ctacagggat ccataaatca caaggaggct gtaaccatcc 661 ccaagggctg cgtcctggcc tttcgagtga gacagctgat ggtcaaaggc aaagatgagt 721 gggatattcc acatatctgc aatgataaca tgcaaacctt ccctcctgga gaaaagtcag 781 gagaggagaa ggtcatcctt atccaggcat ctgatgttgg ggacgtacac gaaggcttca 841 ggacactaaa agaagaagtt cagagagaga cccaacaagt ggagaagctg agccgagtag 901 ggcaaagctc cctgctcagc tccctcagca aacttctagg gaagaaaaag gagctacaag 961 accttgagct cgcacttgaa ggggctctag acaagggaca tgaagtgaac ctggaggcac 1021 tcccaaaaga tgtcctgcta tcaaaggagg ccgtgggcgc catcctctat ttcgttggag 1081 ccctaacaga gctaagtgaa gcccaacaga agctgctggt gaaatccatg gagaaaaaga 1141 tcctacccgt gcagctaaag ctggtggaga gcacgatgga acagaacttc ctgctggata 1201 aagagggtgt tttccccctg caacctgagc tgctctcctc ccttggggac gaggagctga 1261 ccctcacgga ggctctagtc gggctgagtg gcctggaagt gcagagatcg ggcccccaat 1321 atatgtggga cccagacacc ctccctcgcc tctgtgctct ttatgcaggc ctctctctcc 1381 ttcagcagct taccaaggcc tccttgccaa ctttcttgta caaagtggtt ggtaagccta 1441 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1501 gcgttaagtc gacaatcaac ctctggatta