Construct: ORF ccsbBroad304_10090
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017172.1_s304c1, BRDN0000400661
- Derived from:
- ccsbBroadEn_10090
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KMT5A (387893)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10090
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_020382.7 | 99.8% | 41.4% | 432delT;947C>G |
| 2 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001367386.1 | 98.1% | 39.7% | 0_1insATGGCTAGAGGCAGGAAG;414delT;929C>G |
| 3 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001324504.2 | 91.2% | 32.9% | 26_27ins90;342delT;857C>G |
| 4 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001367388.1 | 88.3% | 28.8% | (many diffs) |
| 5 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001324505.2 | 83.6% | 25.2% | 0_1ins171;261delT;776C>G |
| 6 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001324506.1 | 69.1% | 10.7% | 0_1ins324;108delT;623C>G |
| 7 | human | 387893 | KMT5A | lysine methyltransferase 5A | NM_001367389.1 | 69.1% | 10.7% | 0_1ins324;108delT;623C>G |
| 8 | human | 387893 | KMT5A | lysine methyltransferase 5A | NR_136910.2 | 36.4% | (many diffs) | |
| 9 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | NM_030241.3 | 89% | 34.4% | (many diffs) |
| 10 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | NM_001310723.1 | 81.4% | 24.8% | (many diffs) |
| 11 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | NM_001310725.1 | 74.1% | 20.5% | (many diffs) |
| 12 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | NM_001310727.1 | 74.1% | 20.5% | (many diffs) |
| 13 | mouse | 67956 | Kmt5a | lysine methyltransferase 5A | XM_006530450.3 | 74.1% | 20.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 534
- ORF length:
- 468
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tagaggcagg aagatgtcca agccccgcgc ggtggaggcg gcggcggcgg 121 cggcggcggt ggcagcgacg gccccgggcc cggagatggt ggagcggagg ggcccgggga 181 ggccccgcac cgacggggag aacgtattta ccgggcagtc aaagatctat tcctacatga 241 gcccgaacaa atgctctgga atgcgtttcc cccttcagga agagaactca gttacacatc 301 acgaagtcaa atgccagggg aaaccattag ccggaatcta caggaaacga gaagagaaaa 361 gaaatgctgg gaacgcagta cggagcgcca tgaagtccga ggaacagaag atcaaagacg 421 ccaggaaagg tcccctggta ccttttccaa accaaaaatc TGAAGCAGCA GAACCTCCAA 481 AAACTCCACC CTCATCTGTG ATTCCACCAA TGCAGCCATC GCCAAGCAAG CCCTGAAAAA 541 GCCCATCAAG GGCAAACAGG CCCCCCGAAA AAAAGCTCAA GGAAAAACGC AACAGAATCG 601 CAAACTTACG GATTTCTACC CTGTCCGAAG GAGCTCCAGG AAGAGCAAAG CCGAGCTGCA 661 GTCTGAAGAA AGGAAAAGAA TAGATGAATT GATTGAAAGT GGGAAGGAAG AAGGAATGAA 721 GATTGACCTC ATCGATGGCA AAGGCAGGGG TGTGATTGCC ACCAAGCAGT TCTCCCGGGG 781 TGACTTTGTG GTGGAATACC ACGGGGACCT CATCGAGATC ACCGACGCCA AGAAACGGGA 841 GGCTCTGTAC GCACAGGACC CTTCCACGGG CTGCTACATG TACTATTTTC AGTATCTGAG 901 CAAAACCTAC TGCGTGGATG CAACTAGAGA GACAAATCGC CTAGGAAGAC TGATCAATCA 961 CAGCAAATGT GGGAACTGCC AAACCAAACT GCACGACATC GACGGCGTAC GTCACCTCAT 1021 CCTCATCGCC TCCCGAGACA TCGCGGCTGG GGAGGAGCTC CTGTATGACT ATGGGGACCG 1081 CAGCAAGGCT TCCATTGAAG CCCACCCGTG GCTGAAGCAT TACCCAACTT TCTTGTACaa 1141 agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc 1201 tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta