Construct: ORF ccsbBroad304_10159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004785.1_s304c1, BRDN0000410700
- Derived from:
- ccsbBroadEn_10159
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H3-5 (440093)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10159
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440093 | H3-5 | H3.5 histone | NM_001013699.3 | 99.7% | 99.2% | 263C>T |
| 2 | human | 3021 | H3-3B | H3.3 histone B | NM_005324.5 | 96.3% | 94.8% | (many diffs) |
| 3 | human | 8355 | H3C8 | H3 clustered histone 8 | NM_003534.2 | 79.1% | 92.6% | (many diffs) |
| 4 | human | 333932 | H3C15 | H3 clustered histone 15 | NM_001005464.2 | 78.6% | 93.3% | (many diffs) |
| 5 | human | 126961 | H3C14 | H3 clustered histone 14 | NM_021059.2 | 78.6% | 93.3% | (many diffs) |
| 6 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001355409.3 | 78.2% | 90.4% | (many diffs) |
| 7 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001372105.1 | 78.2% | 90.4% | (many diffs) |
| 8 | human | 3020 | H3-3A | H3.3 histone A | NM_002107.6 | 76.7% | 94.8% | (many diffs) |
| 9 | mouse | 15081 | H3f3b | H3 histone, family 3B | NM_008211.3 | 89.2% | 94.8% | (many diffs) |
| 10 | mouse | 15081 | H3f3b | H3 histone, family 3B | XM_006532248.2 | 89.2% | 94.8% | (many diffs) |
| 11 | mouse | 97114 | Hist2h3c2 | histone cluster 2, H3c2 | NM_054045.4 | 80.8% | 93.3% | (many diffs) |
| 12 | mouse | 15077 | Hist2h3c1 | histone cluster 2, H3c1 | NM_178216.3 | 80.8% | 93.3% | (many diffs) |
| 13 | mouse | 319150 | Hist1h3b | histone cluster 1, H3b | NM_178203.2 | 80.6% | 93.3% | (many diffs) |
| 14 | mouse | 319149 | Hist1h3d | histone cluster 1, H3d | NM_178204.2 | 80.6% | 93.3% | (many diffs) |
| 15 | mouse | 319154 | Hist2h3b | histone cluster 2, H3b | NM_178215.2 | 80.6% | 93.3% | (many diffs) |
| 16 | mouse | 260423 | Hist1h3f | histone cluster 1, H3f | NM_013548.4 | 80.3% | 93.3% | (many diffs) |
| 17 | mouse | 319152 | Hist1h3h | histone cluster 1, H3h | NM_178206.2 | 80.3% | 92.6% | (many diffs) |
| 18 | mouse | 319151 | Hist1h3e | histone cluster 1, H3e | NM_178205.2 | 80.1% | 93.3% | (many diffs) |
| 19 | mouse | 319148 | Hist1h3c | histone cluster 1, H3c | NM_175653.2 | 80% | 93.3% | (many diffs) |
| 20 | mouse | 360198 | Hist1h3a | histone cluster 1, H3a | NM_013550.5 | 79.9% | 92.6% | (many diffs) |
| 21 | mouse | 97908 | Hist1h3g | histone cluster 1, H3g | NM_145073.2 | 79.6% | 92.6% | (many diffs) |
| 22 | mouse | 319153 | Hist1h3i | histone cluster 1, H3i | NM_178207.2 | 79.1% | 92.6% | (many diffs) |
| 23 | mouse | 624251 | Gm6485 | predicted gene 6485 | XR_375938.3 | 38.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgaaccaag cagactgctc gtaaatccac cggtgggaaa gccccccgca 121 aacagctggc cacgaaagct gccaggaaaa gcaccccctc tacctgcggg gtgaagcctc 181 atcgctacag gcctgggacc gtggcgcttc gagagattcg tcgttatcag aagtcgaccg 241 agctgctcat ccggaagctg cccttccaga ggttggtgag ggagatcgcg caggatttca 301 acactgacct gaggtttcag agcgcagtcg tcggtgcgct gcaggaggct agcgaagcgt 361 acctggtggg tctgttggaa gatactaacc tgtgtgccat ccacgctaag agagtcacca 421 tcatgcccaa agacatccag ttggctcgcc ggatacgggg agagagagct tacccaactt 481 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 541 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta