Transcript: Mouse NM_008211.3

Mus musculus H3 histone, family 3B (H3f3b), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
H3f3b (15081)
Length:
1993
CDS:
137..547

Additional Resources:

NCBI RefSeq record:
NM_008211.3
NBCI Gene record:
H3f3b (15081)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008211.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000092918 CCACAGTGTAACAGTTTCCTT pLKO.1 968 3UTR 100% 3.000 2.100 N H3f3b n/a
2 TRCN0000309364 CCACAGTGTAACAGTTTCCTT pLKO_005 968 3UTR 100% 3.000 2.100 N H3f3b n/a
3 TRCN0000092922 CCACGCCAAGAGAGTCACCAT pLKO.1 475 CDS 100% 0.880 0.528 N H3f3b n/a
4 TRCN0000296064 GACTTGTTGGGTAGCTATTAA pLKO_005 816 3UTR 100% 15.000 7.500 Y H3-3B n/a
5 TRCN0000311286 GAGATCGCCCAGGATTTCAAA pLKO_005 356 CDS 100% 5.625 2.813 Y H3f3b n/a
6 TRCN0000092921 GAAGCTGCCATTCCAGAGATT pLKO.1 328 CDS 100% 4.950 2.475 Y H3f3b n/a
7 TRCN0000309297 GAAGCTGCCATTCCAGAGATT pLKO_005 328 CDS 100% 4.950 2.475 Y H3f3b n/a
8 TRCN0000305377 TCAGGAGGCTAGCGAAGCATA pLKO_005 415 CDS 100% 4.950 2.475 Y H3f3b n/a
9 TRCN0000092920 TCTGAGAGAGATCCGTCGTTA pLKO.1 280 CDS 100% 4.950 2.475 Y H3f3b n/a
10 TRCN0000309299 TCTGAGAGAGATCCGTCGTTA pLKO_005 280 CDS 100% 4.950 2.475 Y H3f3b n/a
11 TRCN0000062391 CATGCCCAAAGACATCCAGTT pLKO.1 496 CDS 100% 4.050 2.025 Y H3-3B n/a
12 TRCN0000298828 CATGCCCAAAGACATCCAGTT pLKO_005 496 CDS 100% 4.050 2.025 Y H3-3B n/a
13 TRCN0000092919 GAGGTTTCAAAGTGCAGCCAT pLKO.1 385 CDS 100% 2.640 1.320 Y H3f3b n/a
14 TRCN0000242127 GTGAAGAAGCCTCACCGCTAC pLKO_005 242 CDS 100% 0.750 0.375 Y Hist1h3d n/a
15 TRCN0000248204 TGAAGAAGCCTCACCGCTACC pLKO_005 243 CDS 100% 0.750 0.375 Y Hist1h3b n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008211.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00719 pDONR223 100% 91.9% 100% None (many diffs) n/a
2 ccsbBroad304_00719 pLX_304 0% 91.9% 100% V5 (many diffs) n/a
3 TRCN0000473475 ACAACCTAGAGAAGGGCCGCGTGA pLX_317 92% 91.9% 100% V5 (many diffs) n/a
4 ccsbBroadEn_10159 pDONR223 100% 89.2% 94.8% None (many diffs) n/a
5 ccsbBroad304_10159 pLX_304 0% 89.2% 94.8% V5 (many diffs) n/a
6 ccsbBroadEn_01907 pDONR223 100% 80.5% 96.3% None (many diffs) n/a
7 ccsbBroad304_01907 pLX_304 0% 80.5% 96.3% V5 (many diffs) n/a
8 TRCN0000469614 CTGCCGATCGGACTGCTGGCTACG pLX_317 100% 80.5% 96.3% V5 (many diffs) n/a
9 ccsbBroadEn_07225 pDONR223 100% 79.1% 95.5% None (many diffs) n/a
10 ccsbBroad304_07225 pLX_304 0% 79.1% 95.5% V5 (many diffs) n/a
11 ccsbBroadEn_04820 pDONR223 100% 78.8% 97% None (many diffs) n/a
12 ccsbBroad304_04820 pLX_304 0% 78.8% 97% V5 (many diffs) n/a
13 TRCN0000470519 GCCGATTGAGCTCTTAAAAGGAAC pLX_317 100% 78.8% 97% V5 (many diffs) n/a
14 ccsbBroadEn_02054 pDONR223 100% 78% 96.3% None (many diffs) n/a
15 ccsbBroad304_02054 pLX_304 0% 78% 96.3% V5 (many diffs) n/a
16 TRCN0000474049 ACGAGTAGAGTAGCATGTAAGCCT pLX_317 92% 78% 96.3% V5 (many diffs) n/a
17 ccsbBroadEn_07333 pDONR223 100% 78% 96.3% None (many diffs) n/a
18 ccsbBroad304_07333 pLX_304 0% 78% 96.3% V5 (many diffs) n/a
19 TRCN0000468120 GCAGCACCCCACCATAATCTTTGA pLX_317 96% 78% 96.3% V5 (many diffs) n/a
20 ccsbBroadEn_07332 pDONR223 100% 77.8% 96.3% None (many diffs) n/a
21 ccsbBroad304_07332 pLX_304 0% 77.8% 96.3% V5 (many diffs) n/a
22 TRCN0000475169 CTGATGTGCTCCCTCCTTGCCCTC pLX_317 57.4% 77.8% 96.3% V5 (many diffs) n/a
Download CSV