Construct: ORF ccsbBroad304_10220
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007712.1_s304c1, BRDN0000402667
- Derived from:
- ccsbBroadEn_10220
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AFG3L1P (172)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10220
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 172 | AFG3L1P | AFG3 like matrix AAA peptid... | NR_003228.1 | 18.8% | 1_768del;1135_1941del | |
| 2 | human | 172 | AFG3L1P | AFG3 like matrix AAA peptid... | NR_003227.1 | 11.9% | (many diffs) | |
| 3 | human | 172 | AFG3L1P | AFG3 like matrix AAA peptid... | NR_003226.1 | 11.4% | 1_802del;1169_3186del | |
| 4 | mouse | 114896 | Afg3l1 | AFG3-like AAA ATPase 1 | XM_011248276.2 | 15.8% | 11.6% | (many diffs) |
| 5 | mouse | 114896 | Afg3l1 | AFG3-like AAA ATPase 1 | NM_054070.3 | 13.3% | 9.8% | (many diffs) |
| 6 | mouse | 114896 | Afg3l1 | AFG3-like AAA ATPase 1 | NR_028260.1 | 7.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 432
- ORF length:
- 366
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga atttgtgaat ttcctgaaga atcccaagca gtatcaggac ttaggagcaa 121 aaattccaaa gggagccatg ctcactggtc ctcctggtac cggcaagacc cttcttgcca 181 aagcaactgc aggggaggcc actgtgccct tcatcactgt gaacgggtct gagttcctgg 241 aaatgtttat tggcgttggg ccagcaaggg gttcaactct gccaccaacg tcgtggtgtt 301 ggctggcacc aaccgccctg acgtcctgga cccggccctc atgcggcctg gccggtttca 361 tcgccagatt tacactggtc ccccttacat taaaggcagg tcctccatct ttaaggtcca 421 cctgcgccca ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 481 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 541 caatcaacct ctggatta