Construct: ORF ccsbBroad304_10220

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007712.1_s304c1, BRDN0000402667
Derived from:
ccsbBroadEn_10220
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AFG3L1P (172)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10220
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 172 AFG3L1P AFG3 like matrix AAA peptid... NR_003228.1 18.8% 1_768del;1135_1941del
2 human 172 AFG3L1P AFG3 like matrix AAA peptid... NR_003227.1 11.9% (many diffs)
3 human 172 AFG3L1P AFG3 like matrix AAA peptid... NR_003226.1 11.4% 1_802del;1169_3186del
4 mouse 114896 Afg3l1 AFG3-like AAA ATPase 1 XM_011248276.2 15.8% 11.6% (many diffs)
5 mouse 114896 Afg3l1 AFG3-like AAA ATPase 1 NM_054070.3 13.3% 9.8% (many diffs)
6 mouse 114896 Afg3l1 AFG3-like AAA ATPase 1 NR_028260.1 7.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
432
ORF length:
366
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga atttgtgaat ttcctgaaga atcccaagca gtatcaggac ttaggagcaa
121aaattccaaa gggagccatg ctcactggtc ctcctggtac cggcaagacc cttcttgcca
181aagcaactgc aggggaggcc actgtgccct tcatcactgt gaacgggtct gagttcctgg
241aaatgtttat tggcgttggg ccagcaaggg gttcaactct gccaccaacg tcgtggtgtt
301ggctggcacc aaccgccctg acgtcctgga cccggccctc atgcggcctg gccggtttca
361tcgccagatt tacactggtc ccccttacat taaaggcagg tcctccatct ttaaggtcca
421cctgcgccca ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
481tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
541caatcaacct ctggatta

Download FASTA (ORF) (Full)